LOCUS       sequence-357209-        8642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a 
            pcDNA3.1 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Berlew EE, Yamada K, Kuznetsov IA, Rand EA, Ochs CC, Jaber Z, 
            Gardner KH, Chow BY
  TITLE     Designing Single-Component Optogenetic Membrane Recruitment Systems:
            The Rho-Family GTPase Signaling Toolbox.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):515-521. doi: 
            10.1021/acssynbio.1c00604. Epub 2022 Jan 3.
  PUBMED    34978789
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00604"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "515-521"
COMMENT     Sequence Label: opto-Tiam1
FEATURES             Location/Qualifiers
     source          1..8642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        160..539
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        540..743
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     694..714
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     788..807
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        788..806
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3413..4123
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3558..3576)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3826..3845
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4158..4175)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4164..4388
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4434..4862
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4521..4540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4731..4752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4871..4891)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4876..5205
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5056..5191
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5118..5137
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5272..6066
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5326..5345)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5936..5955
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6240..6361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6277..6296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6331..6350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6410..6426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6410..6426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6423..6445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6434..6450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6458..6464,6465..6482,6483..6488))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6503..6524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6641..6658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6812..7400)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6892..6911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7571..8362,8363..8431))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFHVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8194..8213
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8432..8536)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8611..8630)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
       61 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
      121 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      181 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      241 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      301 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      361 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      421 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      481 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      541 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      601 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      661 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      721 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
      781 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct
      841 tggtaccgag ctcggatcca tggccacaga cgcaatcgac cattactacc agaacagaga
      901 cggcccccct tccaggagct ttagcgacga atacgactca tacaataaga cagcccctct
      961 gccacctctg agcgcctgcc cagagcgcgc ccagacccct aacagcaaga gaatccccat
     1021 cacacccaat tccgccaaga gcacctccga gtctcctcgg agaatcacat tctctcgcga
     1081 cgcaggcagc tccaccaagg gcagcaccca gacaagccca tccaaccagg gctccgtgtc
     1141 tagccggaat ggctctaacg gaaatggcct gggcctgtcc tctgccggca acttcgccga
     1201 tttctttagc tccgaggtgt tttatatcgt gctgcacaat cccaccacag cccacagatt
     1261 cctgaagttt tgccagagca gggcctgttc cgagaacctg gagtttctgc agaagatcga
     1321 cgagtacaat aggctgatcg atgaggccac acgcctgctg tctaccatct acagcacata
     1381 cgtgagcagc gaggccccta agcagatcaa catctctaat agccacgcca ggcgcctgag
     1441 ccacgacatc aagggagcaa cccacaacgt gctgcccggc ctggaggata tcttcaatgg
     1501 ctcccaggag cagatcgaga agctgctggc ctctgacctg tatcctagat ttgtgaagca
     1561 ccaggtgacc gcatccgcca caatggccct ggcaaacgac aaggagaggt acccaggcct
     1621 gggcgattgc ttctgtctga cagacccacg gagagccgat aatcccatcg tgttcgcaag
     1681 cgacggattc gtgagcgtga ccggctattc ccgctctgat atcatcccaa ggaactgccg
     1741 cttcctgcag ggcagcttta ccgaccggca ggccacaaag aggctgcgca cctccatcga
     1801 gaatcgcgag gagacagtgg agctgctgct gaactatcgg aagaatggcg atcccttttg
     1861 gaacctgctg tacgtgtccc ctctgctgga cggcaatggc gatgtgagat tctttctggg
     1921 cggccagatc aactgttcta ccacaatcca cagccggacc gacgtgctga gaatcctgag
     1981 cctgaacgac gaggatacag gctccttcat cgagggctct aataagaccc ccagcgtgcg
     2041 gtccaaggag tctaacaatg cctccaacta tggcaagaca tccttcttta agtcttttaa
     2101 gaagtacaag gccaataccg tggagatcag agacgaggcc ggcatggagg gcgagctgat
     2161 cgataggatc ggcaagctgc agttcaagac acaggtggag gagttttaca ccgcctattc
     2221 taagtacctg gtgctgagct atcagccaca gtcccagaac ctgatcgtga agtacttcag
     2281 ccccggcatc gtggatatgc tgaacctgaa tatgcctaat ggcctggtgg ccccaatcgt
     2341 ggacaaggat atcttcaagg tgctgacaga gcactcccct tcctctgtgc cacggacctt
     2401 taagaacgtg gtgagggaag gcatcaaagc aggcagggcc gtgtctgtgg agacaggcct
     2461 gctgacaggc atcgaggaga tcaagaagtc tcagaacatc ttcagcggca ccacagccaa
     2521 tcgggagaga ggctggaaga aggccgagga gaagtacgtg tgccacttta ccccctgtaa
     2581 ggatgagatg ggcagggtcg gctgggtggt gctgactgtc gctcctaaac tggaaagatt
     2641 tgaattcggt ggcggatcag gaggagggtc aatgaacccc tctgaccaga gcccatctcc
     2701 tcaggactcc acggggcctc agctggcgac catgagacaa ctctcggatg cagataagct
     2761 gcgcaaggtg atctgcgagc tcctggagac ggagcgcacc tacgtgaagg atttaaactg
     2821 tcttatggag agatacctaa agcctcttca aaaagaaact tttctcaccc aggatgagct
     2881 tgacgtgctt tttggaaatt taacggaaat ggtagagttt caagtagaat tccttaaaac
     2941 tctagaagat ggagtgagac tggtacctga tttggaaaag cttgagaagg ttgatcaatt
     3001 taagaaagtg ctgttctctc tggggggatc attcctgtat tatgctgacc gcttcaagct
     3061 ctacagtgcc ttctgcgcca gccacacaaa agttcccaag gtcctggtga aagccaagac
     3121 agacacggct ttcaaggcat tcttggatgc ccagaacccg aagcagcagc actcatccac
     3181 gctggagtcg tacctcatca agcccatcca gaggatcctc aagtacccac ttctgctcag
     3241 ggagctgttc gccctgaccg atgcggagag cgaggagcac taccacctgg acgtggccat
     3301 caagaccatg aacaaggttg ccagtcacat caatgagatg cagaaaatcc atgaagagtt
     3361 tggggctgtg tttgaccagc tgattgctgg tggtggatca ggtggaggat ctatggtgag
     3421 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     3481 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     3541 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     3601 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     3661 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     3721 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     3781 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     3841 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     3901 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     3961 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     4021 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     4081 gggccgccac tccaccggcg gcatggacga gctgtacaag taatgatcta gagggcccgt
     4141 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     4201 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4261 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4321 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4381 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4441 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4501 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4561 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4621 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4681 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4741 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4801 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4861 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4921 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4981 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5041 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5101 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     5161 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     5221 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     5281 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5341 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5401 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5461 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5521 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5581 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5641 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5701 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5761 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5821 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5881 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5941 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6001 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6061 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6121 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6181 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     6241 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6301 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6361 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6421 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6481 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6541 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6601 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6661 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6721 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6781 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6841 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6901 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6961 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7021 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7081 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7141 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7201 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7261 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7321 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7381 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7441 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7501 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7561 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7621 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7681 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7741 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7801 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7861 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7921 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7981 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8041 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8101 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8161 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8221 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8281 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8341 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8401 taagggcgac atggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8461 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8521 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     8581 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     8641 cc
//
