LOCUS       sequence-357211-        4368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor with homologous arms for Rab11a to knock in mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Gemperle J, Harrison TS, Flett C, Adamson AD, Caswell PT
  TITLE     On demand expression control of endogenous genes with DExCon, 
            DExogron and LUXon reveals differential dynamics of Rab11 family 
            members.
  JOURNAL   Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651.
  PUBMED    35708998
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-16"; volume: "11. pii: 76651. doi"; pages: " 
            10.7554/eLife.76651"
COMMENT     Sequence Label: pJET-07-mNeonGreen-R11a
FEATURES             Location/Qualifiers
     source          1..4368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(241..1032,1033..1101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     864..883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1102..1206)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1274..1292
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1598..1616
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2443..2452
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3034..3052)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(3493..3499,3500..3517,3518..3523))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    3538..3559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3679..3696)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3850..4368,1..70))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3930..3949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
       61 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      181 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
      241 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
      301 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      361 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      421 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      481 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      541 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      601 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      661 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      721 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      781 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      841 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      901 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      961 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     1021 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     1081 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     1141 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     1201 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1261 attaacctat aaaaataggc gtatcacgag gccgcccctg cagccgaatt atattatttt
     1321 tgccaaataa tttttaacaa aagctctgaa gtcttcttca tttaaattct tagatgatac
     1381 ttcatctgga aaattgtccc aattagtagc atcacgctgt gagtaagttc taaaccattt
     1441 ttttattgtt gtattatctc taatcttact actcgatgag ttttcggtat tatctctatt
     1501 tttaacttgg agcaggttcc attcattgtt tttttcatca tagtgaataa aatcaactgc
     1561 tttaacactt gtgcctgaac accatatcca tccggcgtaa tacgactcac tatagggaga
     1621 gcggccgcca gatcttccgg atggctcgag tttttcagca agatcgatgt gggagaaggc
     1681 agtccgccag gggaaggaca cgcagggagg ggctctgagg ccagcgaagg cctatggaga
     1741 ggtcgctcag agagcggcat gtgatgggga cccctgtctg agcgggggag aagaggctga
     1801 gctgggtagg agacggaacc gcacaaaaag gaagggctga gggaggccta tatcagtgca
     1861 ggggtggccg gcacgagggt ccaccgggag tggcccgggt ccccgaacga ggactgtgta
     1921 gagtgcgaga gcccatggcc tcacctttaa agaggtagtc gtactcgtcg tcgcgtgtac
     1981 cacttccagc accggttccc ttgtacagct cgtccatgcc catcacgtcg gtgaaggctt
     2041 tctgccactc tttgaagttc agttctgtct tggagtgctt cagctcggtc ttgcggaaca
     2101 cgtacatagg ctggttcttc aggtagttgg cggccatagg tttggcgaag gtgtaggtgg
     2161 ttctggcggt gcttctgtac cgcttgccgt tgccggtggt gtaggaccac ttgaaggtgc
     2221 tgatgatggt cttgtcgttg gggtatgttt tcttgcttct gcaccagtcg gcggctgtca
     2281 gagaattggt catcacgggg ccatcagcag ggaagcctgt gcctttcact tgggcctcgc
     2341 ctttgatgtg gctgccctcg tatgtgtacc ggtagttcac ggtcaggctg gcgccatctt
     2401 caaactgcat ggtccggtgc acctggtagc cagagccatc aaccatggcg gcctggaaag
     2461 ggctcatgcc gtcaggataa ggcaggtact ggtggaagcc gtagccgatg tgaggcacca
     2521 gaatccaggg gctgaactgc aggtcgccct tggtggactt caggttcagt tcctcgtagc
     2581 cgtcgttggg attgccggtg ccttgtccga ccatgtcgaa gtccacgccg ttgatgctgc
     2641 cgaagatgtg cagctcgtgt gtggcaggca ggctggccat attgtcctct tcgcccttag
     2701 acacgccact agtcccaccc gatcccattg cgcggccgag gagcgaaagg gcggagcagc
     2761 agtggtatct gtgggaccag ggggcgtcgc tgcaggggta acccgagcgc cgagcttcaa
     2821 ctgccggact gggtgaagag cccctccctg ccgctgcgcc atttccggca ggccccagcc
     2881 tttaagggga agtacttccg ggggcggcgc cccgcccctg agcccaacaa gggcggtgtg
     2941 cgcgagagtc gagatgcgtg gggcttcaag ctttagtcgc taaaggaggc tcaaagggga
     3001 aactgagtcc tggaactcct agctaccata gtccctatag tgagtcgtat tatcagctat
     3061 ctttctagaa gatctcctac aatattctca gctgccatgg aaaatcgatg ttcttctttt
     3121 attctctcaa gattttcagg ctgtatatta aaacttatat taagaactat gctaaccacc
     3181 tcatcaggaa ccgttgtagg tggcgtgggt tttcttggca atcgactctc atgaaaacta
     3241 cgagctaaat attcaatatg ttcctcttga ccaactttat tctgcatttt ttttgaacga
     3301 ggtttagagc aagcttcagg aaactgagac aggaatttta ttaaaaattt aaattttgaa
     3361 gaaagttcag ggttaatagc atccattttt tgctttgcaa gttcctcagc attcttaaca
     3421 aaagacgtct cttttgacat gtttaaagtt taaacctcct gtgtgaaatt attatccgct
     3481 cataattcca cacattatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgcca attgctttcc agtcgggaaa
     3601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4261 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaaca
//
