LOCUS       sequence-357222-        9305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for lentiviral expression of brachyury ORF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Sheppard HE, Dall'Agnese A, Park WD, Shamim MH, Dubrulle J, Johnson 
            HL, Stossi F, Cogswell P, Sommer J, Levy J, Sharifnia T, Wawer MJ, 
            Nabet B, Gray NS, Clemons PA, Schreiber SL, Workman P, Young RA, Lin
            CY
  TITLE     Targeted brachyury degradation disrupts a highly specific 
            autoregulatory program controlling chordoma cell identity.
  JOURNAL   Cell Rep Med. 2021 Jan 19;2(1):100188. doi: 
            10.1016/j.xcrm.2020.100188. eCollection 2021 Jan 19.
  PUBMED    33521702
REFERENCE   2  (bases 1 to 9305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Med."; date: "2021-01-19"; volume: "2(1):100188. doi"; pages: " 
            10.1016/j.xcrm.2020.100188. eCollection 2021 Jan 19"
COMMENT     Sequence Label: Phage-PGK-Flag-HA-FKBP-T(G177D)
FEATURES             Location/Qualifiers
     source          1..9305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2261..2284)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2611..2630
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2711..2720
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2720..2743
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2756..2782
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(2789..2791,2792..3112)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     protein_bind    3119..3143
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4461..4485)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4498..5065
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4515..5067
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4659..4676)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4886..4905
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5079..5678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5079..5098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5575..5595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5695..6283
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5748..5768)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6166..6177)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6358..6591
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6710..6728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6796..6900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6901..6969,6970..7761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7119..7138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7932..8520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8421..8440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8674..8691
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8931..8952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8967..8972,8973..8990,8991..8997)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9005..9021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9010..9032
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9029..9045
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9029..9045
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9095..9114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcatt
     2221 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     2281 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     2341 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     2401 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     2461 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     2521 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc aggggcgggg
     2581 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     2641 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcggccgc agatctctcg
     2701 aggttgatct gccaccatgg actacaagga tgacgatgac aagctcgatg gaggataccc
     2761 atacgatgtt ccagattacg ctgctagcat gggagtgcag gtggaaacca tctccccagg
     2821 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct
     2881 tgaagatgga aagaaagttg attcctcccg ggacagaaac aagcccttta agtttatgct
     2941 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca
     3001 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat
     3061 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg aaacgcgtac
     3121 aagtttgtac aaaaaagcag gcttcaccat gagctcccct ggcaccgaga gcgcgggaaa
     3181 gagcctgcag taccgagtgg accacctgct gagcgccgtg gagaatgagc tgcaggcggg
     3241 cagcgagaag ggcgacccca cagagcgcga actgcgcgtg ggcctggagg agagcgagct
     3301 gtggctgcgc ttcaaggagc tcaccaatga gatgatcgtg accaagaacg gcaggaggat
     3361 gtttccggtg ctgaaggtga acgtgtctgg cctggacccc aacgccatgt actccttcct
     3421 gctggacttc gtggcggcgg acaaccaccg ctggaagtac gtgaacgggg aatgggtgcc
     3481 ggggggcaag ccggagccgc aggcgcccag ctgcgtctac atccaccccg actcgcccaa
     3541 cttcggggcc cactggatga aggctcccgt ctccttcagc aaagtcaagc tcaccaacaa
     3601 gctcaacgga gggggccaga tcatgctgaa ctccttgcat aagtatgagc ctcgaatcca
     3661 catagtgaga gttggggatc cacagcgcat gatcaccagc cactgcttcc ctgagaccca
     3721 gttcatagct gtgactgctt atcagaacga ggagatcaca gctcttaaaa ttaagtacaa
     3781 tccatttgca aaagctttcc ttgatgcaaa ggaaagaagt gatcacaaag agatgatgga
     3841 ggaacccgga gacagccagc aacctgggta ctcccaatca ggggggtggc ttcttcctgg
     3901 aaccagcacc ctgtgtccac ctgcaaatcc tcatcctcag tttggaggtg ccctctccct
     3961 cccctccacg cacagctgtg acaggtaccc aaccctgagg agccaccggt cctcacccta
     4021 ccccagcccc tatgctcatc ggaacaattc tccaacctat tctgacaact cacctgcatg
     4081 tttatccatg ctgcaatccc atgacaattg gtccagcctt ggaatgcctg cccatcccag
     4141 catgctcccc gtgagccaca atgccagccc acctaccagc tccagtcagt accccagcct
     4201 gtggtctgtg agcaacggcg ccgtcacccc gggctcccag gcagcagccg tgtccaacgg
     4261 gctgggggcc cagttcttcc ggggctcccc cgcgcactac acacccctca cccatccggt
     4321 ctcggcgccc tcttcctcgg gatccccact gtacgaaggg gcggccgcgg ccacagacat
     4381 cgtggacagc cagtacgacg ccgcagccca aggccgcctc atagcctcat ggacacctgt
     4441 gtcgccacct tccatgtgag acccagcttt cttgtacaaa gtggttcgat aacgaattcc
     4501 gccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     4561 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     4621 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     4681 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     4741 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     4801 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     4861 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     4921 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     4981 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     5041 tttcctttga aaaacacgat gataatatgg ccacaaccat gaccgagtac aagcccacgg
     5101 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     5161 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     5221 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     5281 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     5341 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     5401 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     5461 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     5521 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     5581 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     5641 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatg catcgatgga tcctaatcaa
     5701 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5761 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5821 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5881 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5941 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6001 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6061 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6121 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6181 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6241 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac
     6301 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6361 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6421 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6481 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6541 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     6601 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6661 ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     6721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     6781 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6841 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6901 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6961 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7021 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7081 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7141 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7201 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7261 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7321 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7381 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7441 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7501 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7561 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7621 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7681 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7741 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7801 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7861 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7921 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7981 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8041 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     8101 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8161 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8221 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8281 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8341 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8401 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8461 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8521 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8581 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8641 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8701 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
     8761 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8821 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
     8881 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8941 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9001 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     9061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9121 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     9181 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     9241 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     9301 gtagt
//
