LOCUS       sequence-35725-e        8039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Chu BW, Banaszynski LA, Chen LC, Wandless TJ
  TITLE     Recent progress with FKBP-derived destabilizing domains.
  JOURNAL   Bioorg Med Chem Lett. 2008 Nov 15;18(22):5941-4. Epub 2008 Sep 12.
  PUBMED    18815033
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioorg Med 
            Chem Lett."; date: "2008-11-15"; volume: "18(22)"; pages: "5941-4. 
            Epub 2008 Sep 12"
COMMENT     Sequence Label: pBMN YFP-FKBP(DD)
FEATURES             Location/Qualifiers
     source          1..8039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..594
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    657..1014
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1079..1495
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1425..1447
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1463..1479
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1505..1878
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1816..1832
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1896..1922
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1923..1925,1926..1928,1929..2639)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1968..1989)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2229..2248)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2576..2597
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2646..2969
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLGDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQGAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     ELE"
     misc_feature    join(3009..3582,3583..3585,3586..3594)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3176..3193)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3403..3422
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3595..4278
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     CDS             4291..4977
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     LTR             5026..5619
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5750..5767)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5921..6509)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6001..6020)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6683..7474,7475..7543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7306..7325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     7611..7629
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
      121 agctgaatat gggccaaagc ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      421 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
      481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
      541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
      601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
      841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg cacccccctt
      901 agaggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaata tgggcccggg
     1081 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
     1141 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
     1201 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
     1261 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
     1321 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
     1381 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
     1441 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgcccccat
     1501 atggccatat gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga
     1561 catgacaaga gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt
     1621 ccagcacgaa gtctggagac ctctggcggc acgtaccaag aacaactgga ccgaccggtg
     1681 gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac
     1741 ctagaacctc gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa
     1801 gtagacggca tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt
     1861 ggaccatcct ctagactgcc ggatccgcca ccatgtatcc gtacgacgta ccggattatg
     1921 cgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1981 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2041 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2101 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
     2161 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2221 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2281 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2341 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2401 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2461 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2521 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2581 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     2641 aattcggagt gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg
     2701 gccagacctg cgtggtgcac tacaccggga tgcttggaga tggaaagaaa gttgactcct
     2761 cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct
     2821 gggaagaagg ggttgcccag atgagtgtgg gtcagggagc caaactgact atatctccag
     2881 attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct
     2941 tcgatgtgga gcttctagaa ctggaatgac tcgagggccg gcgcgccgcg gccgctacgt
     3001 aaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     3061 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     3121 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     3181 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     3241 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     3301 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     3361 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     3421 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     3481 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga
     3541 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg
     3601 agcggcctgc tgaaggagag tatgcgcatc aagatgtaca tggagggcac cgtgaacggc
     3661 cactacttca agtgcgaggg cgagggcgac ggcaacccct tcgccggcac ccagagcatg
     3721 agaatccacg tgaccgaggg cgcccccctg cccttcgcct tcgacatcct ggccccctgc
     3781 tgcgagtacg gcagcaggac cttcgtgcac cacaccgccg agatccccga cttcttcaag
     3841 cagagcttcc ccgagggctt cacctgggag agaaccacca cctacgagga cggcggcatc
     3901 ctgaccgccc accaggacac cagcctggag ggcaactgcc tgatctacaa ggtgaaggtg
     3961 cacggcacca acttccccgc cgacggcccc gtgatgaaga acaagagcgg cggctgggag
     4021 cccagcaccg aggtggtgta ccccgagaac ggcgtgctgt gcggccggaa cgtgatggcc
     4081 ctgaaggtgg gcgaccggca cctgatctgc caccactaca ccagctaccg gagcaagaag
     4141 gccgtgcgcg ccctgaccat gcccggcttc cacttcaccg acatccggct ccagatgctg
     4201 cggaagaaga aggacgagta cttcgagctg tacgaggcca gcgtggcccg gtacagcgac
     4261 ctgcccgaga aggccaacag atctcccggg atggtgagcg gcctgctgaa ggagagtatg
     4321 cgcatcaaga tgtacatgga gggcaccgtg aacggccact acttcaagtg cgagggcgag
     4381 ggcgacggca accccttcgc cggcacccag agcatgagaa tccacgtgac cgagggcgcc
     4441 cccctgccct tcgccttcga catcctggcc ccctgctgcg agtacggcag caggaccttc
     4501 gtgcaccaca ccgccgagat ccccgacttc ttcaagcaga gcttccccga gggcttcacc
     4561 tgggagagaa ccaccaccta cgaggacggc ggcatcctga ccgcccacca ggacaccagc
     4621 ctggagggca actgcctgat ctacaaggtg aaggtgcacg gcaccaactt ccccgccgac
     4681 ggccccgtga tgaagaacaa gagcggcggc tgggagccca gcaccgaggt ggtgtacccc
     4741 gagaacggcg tgctgtgcgg ccggaacgtg atggccctga aggtgggcga ccggcacctg
     4801 atctgccacc actacaccag ctaccggagc aagaaggccg tgcgcgccct gaccatgccc
     4861 ggcttccact tcaccgacat ccggctccag atgctgcgga agaagaagga cgagtacttc
     4921 gagctgtacg aggccagcgt ggcccggtac agcgacctgc ccgagaaggc caactgagtc
     4981 gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     5041 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
     5101 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
     5161 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
     5221 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     5281 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
     5341 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     5401 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     5461 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
     5521 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     5581 tctgagtgat tgactacccg tcagcggggg tctttcattc acatgcagca tgtatcaaaa
     5641 ttaatttggt tttttttctt aagtatttac attaaatggc catagttgca ttaatgaatc
     5701 ggccaacgcg cggggagagg cggtttgcgt attggcgctc ttccgcttcc tcgctcactg
     5761 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5821 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5881 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5941 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6001 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6061 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6121 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6181 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6241 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6301 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6361 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6421 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6481 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6541 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6601 tcttcaccta gatcctttta aattaaaaat gaagtttgcg caaatcaatc taaagtatat
     6661 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6721 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6781 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6841 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6901 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6961 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7021 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7081 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7141 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7201 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7261 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     7321 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7381 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7441 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7501 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7561 attattgaag catttatcag ggttattgtc tcatgacatt aacctataaa aataggcgta
     7621 tcacgaggcc ctttcgtctt caagaacagc tttgctctta ggagtttcct aatacatccc
     7681 aaactcaaat atataaagca tttgacttgt tctatgccct agggggcggg gggaagctaa
     7741 gccagctttt tttaacattt aaaatgttaa ttccatttta aatgcacaga tgtttttatt
     7801 tcataagggt ttcaatgtgc atgaatgctg caatattcct gttaccaaag ctagtataaa
     7861 taaaaataga taaacgtgga aattacttag agtttctgtc attaacgttt ccttcctcag
     7921 ttgacaacat aaatgcgctg ctgagaagcc agtttgcatc tgtcaggatc aatttcccat
     7981 tatgccagtc atattaatta ctagtcaatt agttgatttt tatttttgac atatacatg
//
