LOCUS       sequence-357267-        8265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TetOn3G transcription factor selectable by hygromycin 
            resistance (Lentiviral vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Gemperle J, Harrison TS, Flett C, Adamson AD, Caswell PT
  TITLE     On demand expression control of endogenous genes with DExCon, 
            DExogron and LUXon reveals differential dynamics of Rab11 family 
            members.
  JOURNAL   Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651.
  PUBMED    35708998
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-16"; volume: "11. pii: 76651. doi"; pages: " 
            10.7554/eLife.76651"
COMMENT     Sequence Label: pCDH-EF1a-HygroR-T2A-TetOn3G
FEATURES             Location/Qualifiers
     source          1..8265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             8..61
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             68..814
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    821..1409
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(874..894)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1292..1303)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1483..1716
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1788..1909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1825..1844)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1879..1898
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1949..2084
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2117..2133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2117..2133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2130..2152)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2141..2157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2165..2171,2172..2189,2190..2195))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2210..2231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2348..2365)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2519..3107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(3278..4069,4070..4138))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3901..3920
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4139..4243)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4311..4329
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4367..4389)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4489..4508
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4702..4724
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4716..4733
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4717..4733
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4748..4974
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4975..5155
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5202..5327
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5820..6053
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6238..6282
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6549..6665
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6710..6921
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6934..7202
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7241..8263
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7241..8263
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 ggccgctgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
       61 ttccggaatg agccggctgg acaagagcaa agtgatcaac agcgccctgg aactgctgaa
      121 cggcgtgggc atcgagggcc tgaccacaag aaagctggcc cagaaactgg gcgtggaaca
      181 gcccaccctg tactggcacg tgaagaacaa gcgggccctg ctggacgccc tgcccatcga
      241 gatgctggac agacaccaca cccacagctg ccccctggaa ggcgagagct ggcaggactt
      301 cctgcggaac aacgccaaga gctacagatg cgccctgctg agccacaggg atggcgccaa
      361 agtgcacctg ggcaccagac ccaccgagaa gcagtacgag acactggaaa accagctggc
      421 cttcctgtgc cagcagggat tcagcctgga aaacgccctg tacgccctga gcgccgtggg
      481 ccactttaca ctgggctgcg tgctggaaga acaggaacac caggtggcca aagaggaaag
      541 agagacaccc accaccgact ccatgccccc cctgctgaag caggccatcg agctgttcga
      601 tagacagggc gccgagcccg cctttctgtt cggactggaa ctgatcatct gcgggctgga
      661 aaagcagctg aagtgcgagt ctggcggccc taccgacgcc ctggacgact tcgatctgga
      721 catgctgcct gccgatgccc tggatgattt tgacctggat atgctgcccg ctgacgctct
      781 ggacgatttc gacctggaca tgctgcccgg ataggtcgac aatcaacctc tggattacaa
      841 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
      901 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
      961 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     1021 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     1081 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     1141 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     1201 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     1261 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     1321 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     1381 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg
     1441 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     1501 cccaacgaag ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     1561 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1621 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1681 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     1741 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     1801 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1861 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     1921 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1981 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     2041 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga
     2101 ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2161 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2221 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2281 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2341 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2401 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2461 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2701 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2941 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3241 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3301 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3361 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3421 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3481 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3541 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3601 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3661 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3721 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3781 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3841 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3901 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3961 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4021 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4081 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4141 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4201 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4261 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4321 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     4381 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     4441 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     4501 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4561 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     4621 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     4681 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
     4741 tgacgcgtgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     4801 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     4861 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     4921 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     4981 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5041 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5101 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     5161 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     5221 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     5281 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     5341 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     5401 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     5461 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     5521 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     5581 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     5641 aaagtaagac caccgcacag caagcggcca ctgatcttca gacctggagg aggagatatg
     5701 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     5761 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     5821 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     5881 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     5941 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     6001 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     6061 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     6121 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     6181 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     6241 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     6301 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     6361 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     6421 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     6481 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     6541 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     6601 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa
     6661 attttatcga attctaaaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     6721 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     6781 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     6841 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     6901 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     6961 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     7021 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     7081 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     7141 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     7201 tccaagctgt gaccggcgcc taggatccta ggcttttgca atgaaaaagc ctgaactcac
     7261 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     7321 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     7381 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     7441 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     7501 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     7561 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     7621 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     7681 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     7741 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     7801 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     7861 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     7921 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     7981 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     8041 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     8101 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     8161 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     8221 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaagg
//
