LOCUS       sequence-357277-        5181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High eukaryotic dual protein expression shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Shang H, Garretson TA, Kumar CMS, Dieter RF, Cheng XW
  TITLE     Improved pFastBac donor plasmid vectors for higher protein 
            production using the Bac-to-Bac(R) baculovirus expression vector 
            system.
  JOURNAL   J Biotechnol. 2017 Aug 10;255:37-46. doi: 
            10.1016/j.jbiotec.2017.06.397. Epub 2017 Jun 20.
  PUBMED    28645582
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2017-08-10"; volume: "255:37-46. doi"; pages: "
            10.1016/j.jbiotec.2017.06.397. Epub 2017 Jun 20"
COMMENT     Sequence Label: pFastBac-Dual-M2
FEATURES             Location/Qualifiers
     source          1..5181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  143..367
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(434..967)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1156..1161,1162..1178,1179..1184))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    1692..1740
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     1765..1784
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(1876..1985)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        2076..2167
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2092..2109
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     mobile_element  2466..2631
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2815..3269
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2902..2921)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3111..3132
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3295..3399
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3400..3468,3469..4260)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3618..3637)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4431..5019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4920..4939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(5173..5181,1..9)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
       61 atttcacacc gcatagacca gccgcgtaac ctggcaaaat cggttacggt tgagtaataa
      121 atggatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag
      181 atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc
      241 cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt
      301 tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tggtaaagac
      361 tatattcgcg gcgttgtgac aatttaccga acaactccgc ggccgggaag ccgatctcgg
      421 cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc
      481 gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt
      541 agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg
      601 caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac
      661 gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt
      721 cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt
      781 ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag
      841 cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac
      901 ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc
      961 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     1021 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc
     1081 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     1141 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc
     1201 atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt
     1261 ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg
     1321 gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgcccttgct tcaggagatc
     1381 ggtagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc
     1441 atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct
     1501 agtggttggc tacgtacccg tagtggctat ggcagggctt gccgccccga cgttggctgc
     1561 gagccctggg ccttcacccg aacttggggg ttggggtggg gaaaaggaag aaacgcgggc
     1621 gtattggtcc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
     1681 cccgcgttta tgaacaaacg acccaacacc cgtgcgtttt attctgtctt tttattgccg
     1741 tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctcccccatc
     1801 tcccggtacc gcatgctatg catcagctgc tagcaccatg gctcgagatc ccgggtgatc
     1861 aagtcttcgt cgagtgattg taaataaaat gtaatttaca gtatagtatt ttaattaata
     1921 tacaaatgat ttgataataa ttcttattta actataatat attgtgttgg gttgaattaa
     1981 aggtccgtag tagcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     2041 accggattaa cataactttc caaaatgttg tagatatcat ggagataatt aaaatgataa
     2101 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     2161 tataaatatt ggatcccggt ccgaagcgcg cggaattcaa aggcctacgt cgacgagctc
     2221 actagtcgcg gccgctttcg aatctagagc ctgcagtctc gacaagcttc ttgtagcagc
     2281 aatctagctt tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc
     2341 aaaatattat gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac
     2401 gtaaacacgt taaataaagc taggacatat ttaacatcgg gcgtgttact cgactaggag
     2461 atccgaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat
     2521 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt
     2581 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat
     2641 ttttcttcct gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat
     2701 cttcggctac tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa
     2761 tgtttgtaat tgactgaata tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc
     2821 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     2881 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     2941 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3001 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3061 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt
     3121 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3181 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3241 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     3301 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3361 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3421 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3481 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3541 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3601 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3661 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3721 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3781 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3841 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3901 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3961 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4021 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4081 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4141 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4201 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4261 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4321 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4381 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4441 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4501 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4561 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     4621 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4681 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4741 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4801 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4861 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4921 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4981 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5041 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5101 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5161 acgaccgagc gcagcgagtc a
//
