LOCUS       sequence-357283-       11001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso2-del1844-2133.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Boland A, Chen Y, Raisch T, Jonas S, Kuzuoglu-Ozturk D, Wohlbold L, 
            Weichenrieder O, Izaurralde E
  TITLE     Structure and assembly of the NOT module of the human CCR4-NOT 
            complex.
  JOURNAL   Nat Struct Mol Biol. 2013 Nov;20(11):1289-97. doi: 
            10.1038/nsmb.2681. Epub 2013 Oct 13.
  PUBMED    24121232
REFERENCE   2  (bases 1 to 11001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2681";
            journalName: "Nat Struct Mol Biol"; date: "2013-11"; volume: "20"; 
            issue: "11"; pages: "1289-97"
COMMENT     Sequence Label: pT7-EGFP-C1-HsNot1iso2-del1844-2133_T
FEATURES             Location/Qualifiers
     source          1..11001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7789..7910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7826..7845)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7880..7899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7917..8372)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8054..8075)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8266..8285
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8399..8503
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8505..8862
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8505..8696
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8528..8548)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8713..8848
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8897..9691
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8897..9691
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8951..8970)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9561..9580
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9879..9898)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9923..9970
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10299..10887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10788..10807
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctatgaatct tgactcgctc tcgctggcct tgtctcaaat
     1441 cagctacctg gtggacaatt taaccaagaa aaattaccga gccagccagc aggaaataca
     1501 gcatattgtg aatcggcacg gtcctgaggc agacaggcat ttattacgct gcctattttc
     1561 gcatgtggat ttcagtggcg atggtaaaag cagtggcaaa gatttccatc agactcagtt
     1621 tctgattcag gagtgtgcgt tgctgattac aaagccaaat tttatctcga cgctgtccta
     1681 tgccattgat aatccattgc actatcagaa gagtttaaag cctgcacccc acttatttgc
     1741 ccagctgagt aaagtgctca aattaagcaa agtacaagag gtaatttttg gccttgccct
     1801 gttgaattct tccagctcag atcttagagg tttcgctgcc cagtttatca aacagaagct
     1861 tccagatctt ctgcgttctt acattgacgc agacgtcagt ggaaatcaag aaggtggctt
     1921 ccaagatata gcaatagagg tcctacacct cctcctctcc catctcctct ttgggcagaa
     1981 gggagccttt ggagttggac aagaacagat agacgctttt cttaagacgc tgcgcagaga
     2041 ttttccccaa gaacgctgtc ccgtggtgct cgcaccactt ttataccctg aaaaacggga
     2101 cattctaatg gacaggatcc tgcctgattc cggaggggta gctaaaacca tgatggagag
     2161 ctctttggct gatttcatgc aagaagtagg ctatggcttt tgtgcaagta ttgaagaatg
     2221 tcgcaatata atcgtgcagt ttggtgttcg ggaggtcaca gctgcccagg ttgcaagggt
     2281 tttgggaatg atggctcgaa ctcattcagg attaacagat ggcattccat tacagagtat
     2341 ttctgctccg ggcagtggga tctggagtga tgggaaagat aaaagtgatg gagcacaggc
     2401 acacacatgg aatgtagaag tcttgattga cgttcttaaa gaactgaatc caagtttgaa
     2461 tttcaaggaa gtaacttatg aactggacca tcctggattt caaattcgtg acagtaaagg
     2521 acttcataat gtggtttatg gcattcagag gggtttgggt atggaagtgt tcccagtaga
     2581 cctcatatat agaccttgga aacatgctga aggccagctc tccttcattc aacattccct
     2641 tataaatcca gagatcttct gttttgctga ctatccctgt catactgttg ccactgatat
     2701 tctgaaagca ccaccagagg atgacaatcg agaaattgcc acatggaaga gcttggattt
     2761 gattgaatct ctgctgaggc ttgcagaggt tgggcagtat gagcaagtca aacagctctt
     2821 cagcttccct atcaaacact gtccagacat gctggtattg gccttactac aaattaacac
     2881 ctcttggcat accttgcgcc atgaacttat ctccactctg atgccaattt tccttggaaa
     2941 ccatcctaac tcagctatta ttttgcacta tgcatggcat gggcagggac agtctccctc
     3001 aattcgccaa cttatcatgc atgcaatggc agaatggtac atgagagggg agcagtatga
     3061 tcaggccaaa ttgtctcgaa tacttgatgt ggcccaggac ttgaaggcct tgtcaatgct
     3121 gctaaatggt actccatttg cctttgttat tgaccttgct gcacttgctt cacgtcgtga
     3181 atacctcaaa cttgataagt ggctcacaga taaaattcga gagcatgggg agccttttat
     3241 ccaggcgtgt atgacttttt taaagagacg gtgtccttct attttgggcg gacttgcccc
     3301 agaaaaagac cagcccaaaa gtgctcaact tcctccagaa actttggcga caatgttggc
     3361 ctgtctgcaa gcttgtgcag ggagtgtttc tcaggagcta tcagaaacta tcctcaccat
     3421 ggtagccaat tgcagtaatg ttatgaataa ggccagacaa ccaccacctg gagttatgcc
     3481 aaaaggacgt cctcctagtg ctagcagctt agatgccatt tctcctgttc agattgaccc
     3541 tcttgctgga atgacatctc ttagtatagg tggttcagct gcccctcaca cccagagtat
     3601 gcagggtttt cctccaaatt tgggttctgc attcagtacc cctcagtcac cagcaaaagc
     3661 atttccaccc ctttcaaccc ccaatcagac cactgcattc agtggtattg gaggactttc
     3721 atcacagctt ccagtaggtg gtcttggcac aggcagcctg actggtatag gaactggtgc
     3781 tcttggactc cctgcagtga ataacgaccc ttttgtacag aggaaactgg gcacctctgg
     3841 actgaatcag cctacattcc agcagacgga cttgtctcag gtgtggccag aggcaaacca
     3901 gcactttagt aaagagatag atgatgaagc aaacagctat ttccagcgaa tatataatca
     3961 tccaccacat ccaaccatgt ctgttgatga ggtattagaa atgctgcaga gatttaaaga
     4021 ctctactata aagagggaac gagaagtatt taactgtatg ctaaggaact tgtttgaaga
     4081 atatcgtttt tttccccagt atcctgataa agagttacat ataacagcct gcctatttgg
     4141 tggtataatt gagaaaggac tggtcactta catggcacta ggtctggctc tacgatatgt
     4201 tcttgaagcc ttacgcaagc cttttggatc caaaatgtat tatttcggga ttgctgcact
     4261 agatagattt aaaaacagat tgaaggacta tccccagtat tgtcagcatt tggcttctat
     4321 cagtcacttt atgcaatttc cacatcattt acaggagtat attgagtatg gacagcagtc
     4381 tagagatcct cctgtgaaaa tgcaaggctc tatcacaacc cctggaagta ttgcactggc
     4441 tcaggcccag gctcaggccc aggttccagc aaaagctcct cttgctggtc aagttagcac
     4501 tatggtaacc acctcaacaa ctaccactgt tgctaaaacg gttacggtca ccaggccaac
     4561 tggagtcagc tttaagaaag atgtgccacc ttctattaat actacaaata tagatacgtt
     4621 gcttgtggcc acagatcaaa ctgagagaat tgtggagccc ccagaaaata tccaggagaa
     4681 aattgctttt attttcaata atctctcaca gtcaaatatg acacaaaagg ttgaagagct
     4741 aaaggaaacg gtgaaagaag aatttatgcc ttgggtttca cagtatctgg ttatgaagag
     4801 agtcagtatt gagccaaact ttcatagcct gtattcaaac ttccttgaca cgctgaagaa
     4861 tcctgaattt aacaagatgg ttctgaatga gacctacaga aacattaaag tgctcctgac
     4921 ctctgataaa gctgcagcca atttctcaga tcgttctttg ctgaagaact tgggacattg
     4981 gctaggaatg atcacattag ctaaaaacaa acccatctta cacactgact tggatgtgaa
     5041 atcattgctg ctagaggctt atgttaaagg acaacaagaa ttgctctatg tagtgccctt
     5101 tgttgccaaa gtcttagaat ctagcattcg tagtgtggtt tttaggccac caaacccttg
     5161 gacaatggca attatgaatg tattagctga gctacatcag gagcatgact taaagttaaa
     5221 cttgaagttt gaaatcgagg ttctctgcaa gaaccttgca ttagacatca atgagctaaa
     5281 acctggaaac ctcctaaagg ataaagatcg cctgaagaat ttagatgagc aactctctgc
     5341 tccaaagaaa gatgtcaagc agccagaaga actccctccc atcacaacca caacaacttc
     5401 tactacacca gctaccaaca ccacttgtac agccacggtt ccaccacagc cacagtacag
     5461 ctaccacgac atcaatgtct attcccttgc gggcttggca ccacacatta ctctaaatcc
     5521 aacaattccc ttgtttcagg cccatccaca gttgaagcag tgtgtgcgtc aggcaattga
     5581 acgggctgtc caggagctgg tccatcctgt ggtggatcga tcaattaaga ttgccatgac
     5641 tacttgtgag caaatagtca ggaaggattt tgccctggat tcggaggaat ctcgaatgcg
     5701 aatagcagct catcacatga tgcgtaactt gacagctgga atggctatga ttacatgcag
     5761 ggaacctttg ctcatgagca tatctaccaa cttaaaaaac agttttgcct cagcccttcg
     5821 tactgcttcc ccacaacaaa gagaaatgat ggatcaggca gctgctcaat tagctcagga
     5881 caattgtgag ttggcttgct gttttattca gaagactgca gtagaaaaag caggccctga
     5941 gatggacaag agattagcaa ctgaatttga gctgagaaaa catgctaggc aagaaggacg
     6001 cagatactgt gatcctgttg ttttaacata tcaagctgaa cggatgccag agcaaatcag
     6061 gctgaaagtt ggtggtgtgg acccaaagca gttggctgtt tacgaagagt ttgcacgcaa
     6121 tgttcctggc ttcttaccta caaatgactt aagtcagccc acgggatttt tagcccagcc
     6181 catgaagcaa gcttgggcaa cagatgatgt agctcagatt tatgataagt gtattacaga
     6241 actggagcaa catctacatg ccatcccacc aactttggcc atgaaccctc aagctcaggc
     6301 tcttcgaagt ctcttggagg ttgtagtttt atctcgaaac tctcgggatg ccatagctgc
     6361 tcttggattg ctccaaaagg ctgtagaggg cttactagat gccacaagtg gtgctgatgc
     6421 tgaccttctg ctgcgctaca gggaatgcca cctcttggtc ctaaaagctc tgcaggatgg
     6481 ccgggcatat gggtctccat ggtgcaacaa acagatcaca aggtgcctaa ttgaatgtcg
     6541 agatgaatat aaatataatg tggaggctgt ggagctgcta attcgcaatc atttggttaa
     6601 tatgcagcag tatgatcttc acctagcgca gtcaatggag aatggcttaa actacatggc
     6661 tgtggcattt gctatgcagt tagtaaaaat cctgctggtg gatgaaagga gtgttgctca
     6721 tgttactgag gcagatctgt tccacaccat tgaaaccctc atgaggatta atgctcattc
     6781 cagaggcaat gctccagaag gattgcccca gctgatggaa gtagtgcgat ccaactatga
     6841 agcaatgatt gatcgtgctc atggaggccc aaactttatg atgcattctg ggatctctca
     6901 agcctcagag tatgatagtg cctttccaag aaacatgagg ctccccgacc cattcactcc
     6961 taatctaaag gtggacatgt tgagtgaaat taacattgct ccccggattc tcaccaattt
     7021 cactggagta atgccacctc agttcaaaaa ggatttggat tcctatctta aaactcgatc
     7081 accagtcact ttcctgtctg atctgcgcag caacctacag gtatccaatg aacctgggaa
     7141 tcgctacaac ctccagctca tcaatgcact ggtgctctat gtcgggactc aggccattgc
     7201 gcacatccac aacaagggca gcacaccttc aatgagcacc atcactcact cagcacacat
     7261 ggatatcttc cagaatttgg ctgtggactt ggacactgag ggtcgctatc tctttttgaa
     7321 tgcaattgca aatcagctcc ggtacccaaa tagccacact cactacttca gttgcaccat
     7381 gctgtacctt tttgcagagg ccaatacgga agccatccaa gaacagatca caagagttct
     7441 cttggaacgg ttgattgtaa ataggccaca tccttggggt cttcttatta ccttcattga
     7501 gctgattaaa aacccagcgt ttaagttctg gaaccatgaa tttgtacact gtgccccaga
     7561 aatcgaaaag ttattccagt cggtcgcaca gtgctgcatg ggacagaagc aggcccagca
     7621 agtaatggaa gggacaggtg ccagttagcc gcgggcccgg gatccaccgg atctagataa
     7681 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     7741 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     7801 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7861 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
     7921 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     7981 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     8041 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     8101 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     8161 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     8221 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     8281 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     8341 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     8401 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8461 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     8521 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     8581 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8641 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     8701 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8761 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8821 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     8881 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     8941 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     9001 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     9061 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     9121 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     9181 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     9241 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     9301 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     9361 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     9421 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     9481 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     9541 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     9601 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     9661 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     9721 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     9781 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     9841 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     9901 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     9961 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
    10021 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
    10081 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
    10141 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    10201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10381 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10441 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10501 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10561 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10621 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10681 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10741 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10801 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10861 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10921 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    10981 aaccgtatta ccgccatgca t
//
