LOCUS       sequence-357324-        4526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor with homologous arms for Rab11b to knock in DExCon-mCherry 
            module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Gemperle J, Harrison TS, Flett C, Adamson AD, Caswell PT
  TITLE     On demand expression control of endogenous genes with DExCon, 
            DExogron and LUXon reveals differential dynamics of Rab11 family 
            members.
  JOURNAL   Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651.
  PUBMED    35708998
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-16"; volume: "11. pii: 76651. doi"; pages: " 
            10.7554/eLife.76651"
COMMENT     Sequence Label: pJET-23-DExCon-mCherry-R11b
FEATURES             Location/Qualifiers
     source          1..4526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(68..859,860..928))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     691..710
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(929..1033)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1101..1119
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1425..1443
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1493..1511
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1824..1842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1860..1878
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1896..1914
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1932..1950
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1968..1986
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2004..2022
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2040..2058
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(join(3478..3484,3485..3502,3503..3508))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    3523..3544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3664..3681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3835..4423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3915..3934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
       61 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
      121 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
      181 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
      241 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
      301 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
      361 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
      421 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
      481 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
      541 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
      601 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
      661 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
      721 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
      781 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
      841 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
      901 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
      961 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     1021 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     1081 tcatgacatt aacctataaa aataggcgta tcacgaggcc gcccctgcag ccgaattata
     1141 ttatttttgc caaataattt ttaacaaaag ctctgaagtc ttcttcattt aaattcttag
     1201 atgatacttc atctggaaaa ttgtcccaat tagtagcatc acgctgtgag taagttctaa
     1261 accatttttt tattgttgta ttatctctaa tcttactact cgatgagttt tcggtattat
     1321 ctctattttt aacttggagc aggttccatt cattgttttt ttcatcatag tgaataaaat
     1381 caactgcttt aacacttgtg cctgaacacc atatccatcc ggcgtaatac gactcactat
     1441 agggagagcg gccgccagat cttccggatg gctcgagttt ttcagcaaga tataatacga
     1501 ctcactatag ggcagtcttc cgccccgccc tcttgagctc gcccctgata ggctggcgcg
     1561 tccgtcactt caaaaaggtc cgcattcctt ccgcctttct ccaggacacc gagggcgagg
     1621 agggtggtac caagcggcgc ccaccctcag agcactactt ccatctctga ttggcttcgc
     1681 tgggtgcccg tcgctactcc actcgccgct gataggccat tcaaccgccg ctctgcgcgg
     1741 cgccggctcc gcccccgtcg ggtgtttgtg gtggggctgc ggagtcgccg atcccgccgg
     1801 aagcgccagg acaaattctt tactccctat cagtgataga gaacgtatga agagtttact
     1861 ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat
     1921 aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga
     1981 tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact
     2041 ccctatcagt gatagagaac gtataagctt tgcttatgta aaccagggcg cctataaaag
     2101 agtgctgatt ttttgagtaa acttcaattc cacaacactt ttgtcttata ccaactttcc
     2161 gtaccacttc ctaccctcgt aaagatggga tcgggtggga ctagtggcat ggtgtccaag
     2221 ggcgaagagg acaacatggc catcatcaaa gaattcatgc ggttcaaggt gcacatggaa
     2281 ggcagcgtga acggccacga gttcgagatt gaaggcgaag gcgagggcag accttacgag
     2341 ggaacacaga ccgccaagct gaaagtgacc aaaggcggcc ctctgccttt tgcctgggac
     2401 attctgagcc ctcagtttat gtacggcagc aaggcctacg tgaagcaccc cgccgatatt
     2461 cccgactacc tgaagctgag cttccccgag ggcttcaagt gggagagagt gatgaacttc
     2521 gaggacggcg gcgtggtcac cgtgactcaa gatagctctc tgcaggacgg cgagttcatc
     2581 tacaaagtga agctgcgggg caccaacttt ccctctgatg gccccgtgat gcagaaaaag
     2641 accatgggct gggaagccag cagcgagaga atgtaccctg aagatggcgc cctgaagggc
     2701 gagatcaagc agcggctgaa actgaaggat ggcggccact acgacgccga agtgaaaacc
     2761 acctacaagg ccaagaaacc cgtgcagctg cctggcgcct acaacgtgaa catcaagctg
     2821 gacatcacca gccacaacga ggactacacc atcgtggaac agtacgagag agccgaaggc
     2881 agacacagca caggcggaat ggacgagctg tacaagggaa ccggtgctgg aagtggtacc
     2941 cgggacgacg agtacgacta cctattcaaa ggtgcggccg gtggggcaca gacgggcgaa
     3001 gtcgtggcgg cgaggcggcg ggcagacggg ccaaggccgc gctatctttc tagaagatct
     3061 cctacaatat tctcagctgc catggaaaat cgatgttctt cttttattct ctcaagattt
     3121 tcaggctgta tattaaaact tatattaaga actatgctaa ccacctcatc aggaaccgtt
     3181 gtaggtggcg tgggttttct tggcaatcga ctctcatgaa aactacgagc taaatattca
     3241 atatgttcct cttgaccaac tttattctgc attttttttg aacgaggttt agagcaagct
     3301 tcaggaaact gagacaggaa ttttattaaa aatttaaatt ttgaagaaag ttcagggtta
     3361 atagcatcca ttttttgctt tgcaagttcc tcagcattct taacaaaaga cgtctctttt
     3421 gacatgttta aagtttaaac ctcctgtgtg aaattattat ccgctcataa ttccacacat
     3481 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3541 attaattgcg ttgcgctcac tgccaattgc tttccagtcg ggaaacctgt cgtgccagct
     3601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3661 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3721 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3781 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3841 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3901 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3961 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4021 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4081 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4141 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4201 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4261 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4321 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4381 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4441 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4501 attatcaaaa aggatcttca cctaga
//
