LOCUS       sequence-357329-        8622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression 
            cassette marked with LEU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Costa EA, Subramanian K, Nunnari J, Weissman JS
  TITLE     Defining the physiological role of SRP in protein-targeting 
            efficiency and specificity.
  JOURNAL   Science. 2018 Jan 18. pii: science.aar3607. doi: 
            10.1126/science.aar3607.
  PUBMED    29348368
REFERENCE   2  (bases 1 to 8622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Jan 18. pii: science.aar3607. doi: 10.1126/science.aar3607."
COMMENT     Sequence Label: pJW1662
FEATURES             Location/Qualifiers
     source          1..8622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             410..2137
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(2196..2214)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      2226..2415
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(2884..3978)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(3979..4386)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(4687..5142)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4824..4845)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5036..5055
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5268..5290
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5282..5299
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5283..5299
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5306..5325
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5306..5324
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    5333..5440
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     5357..5373
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5357..5373
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5407..5423)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5408..5424)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(5452..5470)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5491..5507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5491..5507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5504..5526)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5515..5531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5539..5545,5546..5563,5564..5569))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5584..5605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5722..5739)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5893..6481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5973..5992)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6652..7443,7444..7512))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7275..7294
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7513..7617)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7685..7703
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             7761..7892
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             7899..7925
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      7942..8129
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7997..8021)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(8160..8182)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8282..8301
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(8618..8622,1..397)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca
       61 atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac
      121 agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc
      181 cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact
      241 tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt
      301 attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg
      361 cacaatattt caagctatac caagcataca atcaactatc tcatatacaa tgacgtactt
      421 cccggaggag gtggtggagc acatcttcag cttcctgccg gcgcagcgcg accgcaacac
      481 ggtctcgctc gtctgcaagg tgtggtacga gatcgagagg ctgagccgcc gcggcgtctt
      541 cgtgggcaac tgctacgccg tgcgcgccgg ccgcgtcgcc gcgcggttcc ccaacgtgcg
      601 ggcgctcacg gtgaagggga agccccactt cgccgacttc aacctcgtgc cccccgactg
      661 gggcggctac gcggggccgt ggatcgaggc ggccgcgagg ggatgccacg gcctggagga
      721 gctcaggatg aagcggatgg tggtgtccga cgagagcctc gagctgctgg ctcgctcgtt
      781 cccgcggttc agggctcttg ttcttatcag ctgcgagggg ttcagcactg acgggctagc
      841 cgccgtcgcg agccattgca agcttctgag ggagttggat ttgcaggaaa atgaagtgga
      901 ggatcgaggg cctaggtggc tttcctgctt ccctgattcc tgcacatcac ttgtctcatt
      961 gaattttgcc tgcatcaaag gggaggttaa tgctggttca ctggagagac ttgttagcag
     1021 gtccccaaac ctgcggagtt tgaggctgaa tcgatctgta tcggtagata cacttgcaaa
     1081 gatactactg cgtaccccta acttggagga tttggggaca gggaatttga cagatgactt
     1141 ccaaactgag tcctacttta agcttaccag tgctctggag aaatgcaaga tgttgaggag
     1201 tttgtctgga ttctgggatg cttctcctgt ttgcctgtca tttatctacc ccctgtgtgc
     1261 tcaactgaca ggattgaact tgagctatgc ccccacactt gatgcttctg accttacaaa
     1321 aatgattagc cgctgtgtga agctccaacg cctttgggta ctggattgta tctcggacaa
     1381 aggcttgcaa gtggtggcct ccagttgcaa agacttgcaa gaactcaggg tatttccatc
     1441 agatttctac gtagctggtt attctgcagt gacagaggag ggacttgttg cagtatcctt
     1501 gggctgtcca aaactgaact cactactgta cttctgtcac caaatgacta atgctgcact
     1561 agttactgtc gccaagaact gtccaaattt cacacgattc agactttgta ttcttgagcc
     1621 agggaagcct gatgttgtga caagccaacc attagatgaa ggctttggag ctattgttcg
     1681 tgagtgcaag ggattacaac gtttgtcaat atctggtctt ctcacagaca aagttttcat
     1741 gtatattggg aaatatgcaa aacaacttga gatgctttct atagcatttg ctggtgacag
     1801 tgataagggt atgatgcatg ttatgaatgg atgcaagaat ttaaggaaac tggagataag
     1861 agatagcccg tttggtgatg ctgcactctt ggggaatttt gctaggtacg agacaatgcg
     1921 atccctttgg atgtcatctt gcaatgtcac gttaaagggg tgccaagtcc ttgcgtcaaa
     1981 gatgccgatg ctcaatgttg aggtcataaa tgagcgggat ggtagcaatg aaatggagga
     2041 aaaccatgga gatctgccta aagtggagaa attatatgtg taccgcacaa ctgctggggc
     2101 gagggatgat gcaccaaatt ttgttaaaat cctatgatag gcctatccct aaccctctcc
     2161 tcggtctcga ttctacgtaa catgtaatta gttatgtcac gcttacattc acgccctccc
     2221 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
     2281 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     2341 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
     2401 ttgggacgct cgaaggcttt aggccgtttc tgacagagta aaattcttga gggaactttc
     2461 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
     2521 ttgagtgttt tttatttgtt gtattttttt tttttttaga gaaaatcctc caatatataa
     2581 attaggaatc atagtttcat gattttctgt tacacctaac tttttgtgtg gtgccctcct
     2641 ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga gccattagta
     2701 tcaatttgct tacctgtatt cctttacatc ctcctttttc tccttcttga taaatgtatg
     2761 tagattgcgt atatagtttc gtctacccta tgaacatatt ccattttgta atttcgtgtc
     2821 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc
     2881 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact
     2941 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc
     3001 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga
     3061 caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca
     3121 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg
     3181 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat
     3241 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc
     3301 agaatcaatc aattgatgtt gaaccttcaa tgtagggaat tcgttcttga tggtttcctc
     3361 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat
     3421 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac
     3481 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct
     3541 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc
     3601 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct
     3661 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac
     3721 actaccggta ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt
     3781 ggaggcttcc agcgcctcat ctggaagtgg aacacctgta gcatcgatag cagcaccacc
     3841 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag
     3901 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat
     3961 cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat atattgctga
     4021 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa
     4081 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg
     4141 aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc
     4201 caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt
     4261 ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga
     4321 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc
     4381 acagttaact gcggtcaaga tatttcttga atcaggcgcc ttagaccgct cggccaaaca
     4441 accaattact tgttgagaaa tagagtataa ttatcctata aatataacgt ttttgaacac
     4501 acatgaacaa ggaagtacag gacaattgat tttgaagaga atgtggattt tgatgtaatt
     4561 gttgggattc catttttaat aaggcaataa tattaggtat gtagatatac tagaagttct
     4621 cctcgaccgg tcgatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     4681 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     4741 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     4801 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     4861 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     4921 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     4981 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     5041 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     5101 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     5161 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     5221 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     5281 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
     5341 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
     5401 gcttatcgat accgtcgacc tcgagggggg gcccggtacc agcttttgtt ccctttagtg
     5461 agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5521 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5581 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5641 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5701 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5761 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5821 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5881 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5941 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6001 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6061 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6121 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6181 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6241 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6301 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6361 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6421 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6541 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6601 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7561 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7621 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     7681 taaaaatagg cgtatcacga ggccctttcg tcagcggcag cgagactccc gggacctcag
     7741 agtccgccac acccgaaagt cctaaagatc cagccaaacc tccggccaag gcacaagttg
     7801 tgggatggcc accggtgaga tcataccgga agaacgtgat ggtttcctgc caaaaatcaa
     7861 gcggtggccc ggaggcggcg gcgttcgtga agtccggata cccatacgac gtcccagact
     7921 acgcttaaca cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
     7981 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
     8041 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag
     8101 gtcgctctta ttgaccacac ctctaccggc atcgcgcgtt tcggtgatga cggtgaaaac
     8161 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     8221 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     8281 gcggcatcag agcagattgt actgagagtg caccatatcg actacgtcgt agggtgtaca
     8341 atatggactt cctcttttct ggcaaccaaa cccatacatc gggattccta taataccttc
     8401 gttggtctcc ctaacatgta ggtggcggag gggagatata caatagaaca gataccagac
     8461 aagacataat gggctaaaca agactacacc aattacactg cctcattgat ggtggtacat
     8521 aacgaactaa tactgtagcc ctagacttga tagccatcat catatcgaag tttcactacc
     8581 ctttttccat ttgccatcta ttgaagtaat aataggcgca tg
//
