LOCUS       sequence-357335-        4155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor with homologous arms for Rab11b to knock in mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Gemperle J, Harrison TS, Flett C, Adamson AD, Caswell PT
  TITLE     On demand expression control of endogenous genes with DExCon, 
            DExogron and LUXon reveals differential dynamics of Rab11 family 
            members.
  JOURNAL   Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651.
  PUBMED    35708998
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-16"; volume: "11. pii: 76651. doi"; pages: " 
            10.7554/eLife.76651"
COMMENT     Sequence Label: pJET-10-mCherry-R11b
FEATURES             Location/Qualifiers
     source          1..4155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        373..391
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(1987..1993,1994..2011,2012..2017))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2173..2190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2344..2932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2424..2443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3103..3894,3895..3963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3726..3745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3964..4068)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4136..4154
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tataatacga ctcactatag ggcagtcttc cgccccgccc tcttgagctc
      421 gcccctgata ggctggcgcg tccgtcactt caaaaaggtc cgcattcctt ccgcctttct
      481 ccaggacacc gagggcgagg agggtggtac caagcggcgc ccaccctcag agcactactt
      541 ccatctctga ttggcttcgc tgggtgcccg tcgctactcc actcgccgct gataggccat
      601 tcaaccgccg ctctgcgcgg cgccggctcc gcccccgtcg ggtgtttgtg gtggggctgc
      661 ggagtcgccg atcccgccgg aagcgccagg acaatgggat cgggtgggac tagtggcatg
      721 gtgtccaagg gcgaagagga caacatggcc atcatcaaag aattcatgcg gttcaaggtg
      781 cacatggaag gcagcgtgaa cggccacgag ttcgagattg aaggcgaagg cgagggcaga
      841 ccttacgagg gaacacagac cgccaagctg aaagtgacca aaggcggccc tctgcctttt
      901 gcctgggaca ttctgagccc tcagtttatg tacggcagca aggcctacgt gaagcacccc
      961 gccgatattc ccgactacct gaagctgagc ttccccgagg gcttcaagtg ggagagagtg
     1021 atgaacttcg aggacggcgg cgtggtcacc gtgactcaag atagctctct gcaggacggc
     1081 gagttcatct acaaagtgaa gctgcggggc accaactttc cctctgatgg ccccgtgatg
     1141 cagaaaaaga ccatgggctg ggaagccagc agcgagagaa tgtaccctga agatggcgcc
     1201 ctgaagggcg agatcaagca gcggctgaaa ctgaaggatg gcggccacta cgacgccgaa
     1261 gtgaaaacca cctacaaggc caagaaaccc gtgcagctgc ctggcgccta caacgtgaac
     1321 atcaagctgg acatcaccag ccacaacgag gactacacca tcgtggaaca gtacgagaga
     1381 gccgaaggca gacacagcac aggcggaatg gacgagctgt acaagggaac cggtgctgga
     1441 agtggtaccc gggacgacga gtacgactac ctattcaaag gtgcggccgg tggggcacag
     1501 acgggcgaag tcgtggcggc gaggcggcgg gcagacgggc caaggccgcg ctatctttct
     1561 agaagatctc ctacaatatt ctcagctgcc atggaaaatc gatgttcttc ttttattctc
     1621 tcaagatttt caggctgtat attaaaactt atattaagaa ctatgctaac cacctcatca
     1681 ggaaccgttg taggtggcgt gggttttctt ggcaatcgac tctcatgaaa actacgagct
     1741 aaatattcaa tatgttcctc ttgaccaact ttattctgca ttttttttga acgaggttta
     1801 gagcaagctt caggaaactg agacaggaat tttattaaaa atttaaattt tgaagaaagt
     1861 tcagggttaa tagcatccat tttttgcttt gcaagttcct cagcattctt aacaaaagac
     1921 gtctcttttg acatgtttaa agtttaaacc tcctgtgtga aattattatc cgctcataat
     1981 tccacacatt atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2041 ctaactcaca ttaattgcgt tgcgctcact gccaattgct ttccagtcgg gaaacctgtc
     2101 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2161 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2221 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2281 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2341 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2401 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2461 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2521 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2581 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2641 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2701 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2761 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2821 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2881 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2941 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3001 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3061 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3241 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4081 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4141 gcgtatcacg aggcc
//
