LOCUS       sequence-357348-        7822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN3-D575RL676EG683E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Christie M, Boland A, Huntzinger E, Weichenrieder O, Izaurralde E
  TITLE     Structure of the PAN3 pseudokinase reveals the basis for 
            interactions with the PAN2 deadenylase and the GW182 proteins.
  JOURNAL   Mol Cell. 2013 Aug 8;51(3):360-73. doi: 
            10.1016/j.molcel.2013.07.011.
  PUBMED    23932717
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.07"; journalName: "Mol Cell"; date: 
            "2013-08-8- 8"; volume: "51"; issue: "3"; pages: "360-73"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN3-D575RL676EG683E_U
FEATURES             Location/Qualifiers
     source          1..7822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5128..5169
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5179..5196
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5219..5236)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5269..5403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5293..5312)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5347..5366
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6004..6592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6084..6103)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6763..7554,7555..7623))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7386..7405
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7624..7728)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7796..7814
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttgaattcta tgtgctcgac ttatggactc ttatcttttg agttttcaga
     2761 tacggctgct atggatccaa ttttcttctc gccaacgaat ggaataccgt cagagagtaa
     2821 actcgccact tatatgaatc gacagaatgt cgccacaccg agcggttatg ggctgaataa
     2881 cgggttctca ctacttaatt tggattcgcc cctaaataaa aagtcccagg tcacgccgca
     2941 gtcgcccgag ttcattccaa cgcgccttgg ctcgacgccc aacttctatg caccatatca
     3001 caacgcgccc atgcaattaa gtaatgggct caatggcgtc aatgggttaa cagccgccgc
     3061 agcagcagca gcagcagccg ccgccgccgc cgcagcagca gttccctcct ccacgtcatc
     3121 ggcgtcctcc gcctcggtga ccatcagcaa aacggccaac ggtggcgcct ccatgctcgc
     3181 cctgcaaaag accgcatcca tcgcctctgt aaccatcgca caacagcagc cacagcatcc
     3241 acagaaacag caacagcatc cgccgtcggt gggcggagga gctgtgtccg ccgcctccgc
     3301 ccccattgcc attagtggcg ctccaccgaa tcccgccgca gctccgttcg tcagcagcat
     3361 gtccgctcag acgccgctca aggggagggg cgccatgatg cgccaggaat caccaacggc
     3421 agccatgatt tcgggcgcag ggccaggtga gaagtcgcca ccgcacggga tgacgccgca
     3481 cggagcatcg cccataccat cagcgctgcc caccagcgtg caccaggaga acgtaggcgg
     3541 caccatatac ttctatccga ctgccaacgc acagaacagc cagcccgtcg tcaattccat
     3601 ggtggtggat ggaacgcatc cggctctcca tggcgtgagt gctgtggcgc cgatgagtgc
     3661 gggcgttccg gcggccatga tgtacacggg gcatgtgtat ccgggaccgt cctccaacgt
     3721 ggtcaccatg cagccaaaga cgctgctgga gtcggccttc tttatgcccg acgagatgcg
     3781 cgccgaagtc ctcgcccgca acgagatctc taacctgatc atggacgcag cggaggctgc
     3841 gcagcacgct ctaccgctgg aggtggagaa ctaccatgcc ctgtatccac tggaaccgcc
     3901 ggcgcagccg ttgcacgcca agctcacgtt ccccgccacc acttataggg ccacgcacaa
     3961 cacgacgggc tacaagtact gcctgcgaag aatacatggt ttccgcctgc agtcgaccaa
     4021 gtgcatgacg ctggtggaga tgtggaagaa gctgcagcac acgaatgtgg tacaactgcg
     4081 tgaggtgttc acgacgaaag catttggtga taactcttta gtattagtgt acgactatca
     4141 tcccggctca caaacattac tggccaaata cttcactccg gcgccagaga ccaacgggta
     4201 cactgatcca ttccaaggcg aggcccgccc cttcagtcat aagagtaaca tgcagcgaac
     4261 gagcaatgga ccgctgctac cggaggccac catctggtcg atcatcatgc agctgaccgc
     4321 cggcttaaaa gccatccacc atgccggtct tgcctgcaag gttctggatc ccacaaagat
     4381 tatagtgacg ggcaagcgtg tacggtttag ctcatgctgc atttcggata taacgcaatt
     4441 ccgtccgaat gcatccaatc ccttggcgct ggttaatatg catcagcagg acgatctgac
     4501 cgctttgggt cgcttagtat tagctttggc ctgccgctgt cttcaatccg tgcaacgcga
     4561 caatgtccag tccagcatcg atatggtcac gcgaaactac tccaccgatc tgcgaaactt
     4621 tattgtttac ctcttcacca ccaataatcg tcgctccgtg accgacctga tgccgatgat
     4681 tggtgcccgc ttctatacgc aactggatgc actgcagagc aaaattgata tgcaggagga
     4741 cgaggaagcc aaggagatgg agaacgagcg gttatatcgc attttggtca aacttaatag
     4801 catcaacgag cgacccgact ttaatctgga ctgcacttgg tctgagactg gtgatagata
     4861 catgttgaaa ttattccgtg attatttgtt ccattccgtc actgaggatg gccggccctg
     4921 gctagatcac gcacacattg tacaatgcct caacaagctg gatgctggct ccatagagcg
     4981 cgtgcaactg atgtcccgcg acgagcaatc ggtactcatc gtctcctatg ctgaactcaa
     5041 gaattgtctg gagaacgcct tctccgaact gatgtcaagt gcggccaact gagcggccgc
     5101 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
     5161 gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     5221 tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct cgatcgagga tccagacatg
     5281 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     5341 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     5401 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     5461 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gtcgaccgat
     5521 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     5581 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     5641 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     5701 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     5761 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     5821 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     5881 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     5941 tcagggacag cttcaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6001 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6061 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6121 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6181 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6241 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6301 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6361 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6421 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     6481 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6541 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6601 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6661 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6721 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6781 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6841 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6901 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6961 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7021 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
     7081 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7141 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7201 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7261 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7321 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     7381 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7441 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7501 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7561 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7621 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7681 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7741 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     7801 gcgtatcacg aggccctttc gt
//
