LOCUS       sequence-357405-       10172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral eukaryotic expression vector with E.Coli Dam methylation 
            fused to human Lamin A. Used in DamID experiments to methylate DNA 
            that interacts in the proximity of Lamin A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Meuleman W, Peric-Hupkes D, Kind J, Beaudry JB, Pagie L, Kellis M, 
            Reinders M, Wessels L, van Steensel B
  TITLE     Constitutive nuclear lamina-genome interactions are highly conserved
            and associated with A/T-rich sequence.
  JOURNAL   Genome Res. 2013 Feb;23(2):270-80. doi: 10.1101/gr.141028.112. Epub 
            2012 Nov 2.
  PUBMED    23124521
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.141028.112"; journalName: "Genome Res"; date: "2013-02";
            volume: "23"; issue: "2"; pages: "270-80"
COMMENT     Sequence Label: pLenti GW V5 Eco/Dam hum LaminA
FEATURES             Location/Qualifiers
     source          1..10172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(85..105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        90..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     332..351
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        467..514
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             533..907
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1037..1158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1074..1093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1128..1147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1207..1223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1207..1223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1220..1242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1231..1247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1255..1261,1262..1279,1280..1285))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1300..1321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1438..1455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1609..2197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1689..1708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2368..3159,3160..3228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2991..3010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3229..3333)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3408..3427)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3599..3978
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3980..4178
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4196..4376
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4423..4548
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5041..5274
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5459..5503
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             6946..6987
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    7003..7027
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8783..8807)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             9338..9518
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9544..9561)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9550..9774
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9820..10172,1..76)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9907..9926)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10117..10138
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
       61 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
      481 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
      541 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
      601 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
      661 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
      721 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
      781 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
      841 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
      901 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
      961 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     1021 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1141 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     1201 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1261 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     1321 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1381 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1561 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1621 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1681 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1741 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1801 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1861 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1921 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1981 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2041 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2101 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2161 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2221 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2281 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2341 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2401 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2461 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2521 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2581 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2641 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2701 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2761 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2821 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2881 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2941 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3001 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3061 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3121 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3181 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3241 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3301 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3361 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     3421 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     3481 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     3541 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     3601 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     3661 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     3721 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     3781 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3841 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3901 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3961 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     4021 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     4081 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     4141 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     4201 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4261 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4321 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     4381 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     4441 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     4501 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     4561 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     4621 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     4681 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     4741 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     4801 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     4861 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     4921 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     4981 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     5041 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     5101 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     5161 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     5221 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     5281 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     5341 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     5401 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     5461 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     5521 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     5581 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     5641 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     5701 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cccccgggct
     5761 gcaggaattc agatctcggc cgcatattaa gtgcattgtt ctcgataccg ctaagtgcat
     5821 tgttctcgtt agctcgatgg acaagtgcat tgttctcttg ctgaaagctc gatggacaag
     5881 tgcattgttc tcttgctgaa agctcgatgg acaagtgcat tgttctcttg ctgaaagctc
     5941 agtacccggg agtaccctcg accgccggag tataaataga ggcgcttcgt ctacggagcg
     6001 acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac
     6061 aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt gaatcaatta
     6121 aaagtaacca gcaaccaagt aaatcaactg caactacttg atgatattaa acggcatttg
     6181 cccaagggcg aatgtctggt tgagcctttt gtaggtgccg ggtcggtgtt tctcaacacc
     6241 gacttttctc gttatatcct tgccgatatc aatagcgacc tgatcagtct ctataacatt
     6301 gtgaagatgc gtactgatga gtacgtacag gccgcacgcg agctgtttgt tcccgaaaca
     6361 aattgcgccg aggtttacta tcagttccgc gaagagttca acaaaagcca ggatccgttc
     6421 cgtcgggcgg tactgttttt atatttgaac cgctacggtt acaacggcct gtgtcgttac
     6481 aatctgcgcg gtgagtttaa cgtgccgttc ggccgctaca aaaaacccta tttcccggaa
     6541 gcagagttgt atcacttcgc tgaaaaagcg cagaatgcct ttttctattg tgagtcttac
     6601 gccgatagca tggcgcgcgc agatgatgca tccgtcgtct attgcgatcc gccttatgca
     6661 ccgctgtctg cgaccgccaa ctttacggcg tatcacacaa acagttttac gcttgaacaa
     6721 caagcgcatc tggcggagat cgccgaaggt ctggttgagc gccatattcc agtgctgatc
     6781 tccaatcacg atacgatgtt aacgcgtgag tggtatcagc gcgcaaaatt gcatgtcgtc
     6841 aaagttcgac gcagtataag cagcaacggc ggcacacgta aaaaggtgga cgaactgctg
     6901 gctttgtaca aaccaggagt cgtttcaccc gcgaaaaaaa ccggtggtaa gcctatccct
     6961 aaccctctcc tcggtctcga ttctacgatc gatttcgatc aaacaagttt gtacaaaaaa
     7021 gcaggctccg cggccgcccc cttcaccatg gagaccccgt cccagcggcg cgccacccgc
     7081 agcggggcgc aggccagctc cactccgctg tcgcccaccc gcatcacccg gctgcaggag
     7141 aaggaggacc tgcaggagct caatgatcgc ttggcggtct acatcgaccg tgtgcgctcg
     7201 ctggaaacgg agaacgcagg gctgcgcctt cgcatcaccg agtctgaaga ggtggtcagc
     7261 cgcgaggtgt ccggcatcaa ggccgcctac gaggccgagc tcggggatgc ccgcaagacc
     7321 cttgactcag tagccaagga gcgcgcccgc ctgcagctgg agctgagcaa agtgcgtgag
     7381 gagtttaagg agctgaaagc gcgcaatacc aagaaggagg gtgacctgat agctgctcag
     7441 gctcggctga aggacctgga ggctctgctg aactccaagg aggccgcact gagcactgct
     7501 ctcagtgaga agcgcacgct ggagggcgag ctgcatgatc tgcggggcca ggtggccaag
     7561 cttgaggcag ccctaggtga ggccaagaag caacttcagg atgagatgct gcggcgggtg
     7621 gatgctgaga acaggctgca gaccatgaag gaggaactgg acttccagaa gaacatctac
     7681 agtgaggagc tgcgtgagac caagcgccgt catgagaccc gactggtgga gattgacaat
     7741 gggaagcagc gtgagtttga gagccggctg gcggatgcgc tgcaggaact gcgggcccag
     7801 catgaggacc aggtggagca gtataagaag gagctggaga agacttattc tgccaagctg
     7861 gacaatgcca ggcagtctgc tgagaggaac agcaacctgg tgggggctgc ccacgaggag
     7921 ctgcagcagt cgcgcatccg catcgacagc ctctctgccc agctcagcca gctccagaag
     7981 cagctggcag ccaaggaggc gaagcttcga gacctggagg actcactggc ccgtgagcgg
     8041 gacaccagcc ggcggctgct ggcggaaaag gagcgggaga tggccgagat gcgggcaagg
     8101 atgcagcagc agctggacga gtaccaggag cttctggaca tcaagctggc cctggacatg
     8161 gagatccacg cctaccgcaa gctcttggag ggcgaggagg agaggctacg cctgtccccc
     8221 agccctacct cgcagcgcag ccgtggccgt gcttcctctc actcatccca gacacagggt
     8281 gggggcagcg tcaccaaaaa gcgcaaactg gagtccactg agagccgcag cagcttctca
     8341 cagcacgcac gcactagcgg gcgcgtggcc gtggaggagg tggatgagga gggcaagttt
     8401 gtccggctgc gcaacaagtc caatgaggac cagtccatgg gcaattggca gatcaagcgc
     8461 cagaatggag atgatccctt gctgacttac cggttcccac caaagttcac cctgaaggct
     8521 gggcaggtgg tgacgatctg ggctgcagga gctggggcca cccacagccc ccctaccgac
     8581 ctggtgtgga aggcacagaa cacctggggc tgcgggaaca gcctgcgtac ggctctcatc
     8641 aactccactg gggaagaagt ggccatgcgc aagctggtgc gctcagtgac tgtggttgag
     8701 gacgacgagg atgaggatgg agatgacctg ctccatcacc accacgtgag tggtagccgc
     8761 cgctgaaagg gtgggcgcgc cgacccagct ttcttgtaca aagtggttcg atcgaaccgc
     8821 ggtctagact cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     8881 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     8941 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     9001 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     9061 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     9121 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     9181 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     9241 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     9301 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     9361 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9421 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9481 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     9541 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     9601 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     9661 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     9721 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     9781 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     9841 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     9901 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     9961 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    10021 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    10081 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    10141 caacactcaa ccctatctcg gtctattctt tt
//
