LOCUS       sequence-357410-       13539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13539)
  AUTHORS   Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, 
            Lee TS
  TITLE     Identification and microbial production of a terpene-based advanced 
            biofuel.
  JOURNAL   Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
  PUBMED    21952217
REFERENCE   2  (bases 1 to 13539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-09-27"; volume: "2:483. doi"; pages: " 
            10.1038/ncomms1494"
COMMENT     Sequence Label: pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)
FEATURES             Location/Qualifiers
     source          1..13539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3707..3718
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      4336..4407
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4423..4450
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        join(4663..4668,4669..4685,4686..4692)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    4700..4716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             7739..7750
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      10185..10256
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10272..10299
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(10327..10344)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10461..11006)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(10541..10560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(11120..11214)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(11235..11894)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     11809..11828
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(11895..11997)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(12069..13151)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     13113..13132
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13152..13229)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     13435..13454
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
       61 gaattcaaaa gatctaaagg aggccatcct ggccatgaag aactgtgtga ttgtttctgc
      121 ggtccgcacg gcgatcggca gctttaacgg ctctttagcg agcacctctg caatcgatct
      181 gggtgcgacg gtcattaagg ccgccattga acgcgccaaa atcgacagcc agcacgttga
      241 tgaggtgatc atgggcaatg tgttacaagc cggcctgggt caaaacccag cgcgtcaagc
      301 actgttaaaa tctggtctgg ccgagaccgt gtgtggcttc accgtcaata aggtttgcgg
      361 ctctggcctg aagagcgtgg ccctggcagc acaagcgatt caagccggtc aggcacaaag
      421 catcgttgcg ggtggcatgg agaacatgtc tctggcgccg tacttattag atgccaaagc
      481 ccgcagcggt tatcgcctgg gcgatggtca ggtgtacgac gtcatcttac gcgatggctt
      541 aatgtgcgcg acccacggtt accacatggg tattacggcc gaaaacgtgg cgaaagaata
      601 cggcattacg cgcgagatgc aggatgaatt agcactgcac tctcagcgca aagcagcagc
      661 cgcgatcgag tctggtgcgt ttacggcgga aatcgtgcca gttaacgtgg tcacgcgcaa
      721 gaagacgttc gttttcagcc aggacgagtt cccgaaggca aacagcaccg cggaggcctt
      781 aggtgcctta cgcccagcct ttgacaaagc gggcacggtc accgccggta atgcgagcgg
      841 catcaatgat ggtgcagcgg cactggtcat catggaagag agcgccgcat tagcagcggg
      901 tctgacccca ttagcgcgca ttaaatctta tgccagcggc ggcgtcccac cagccctgat
      961 gggcatgggt ccggtcccag ccacgcaaaa agccctgcaa ttagcgggcc tgcaactggc
     1021 cgacattgat ctgatcgagg cgaacgaggc gtttgcagcg cagttcctgg cggtgggtaa
     1081 gaatctgggc ttcgacagcg agaaagtcaa tgtgaacggt ggcgcgattg cgttaggcca
     1141 tccgattggt gcaagcggcg cacgcatctt agtgacgtta ctgcacgcca tgcaggcacg
     1201 cgacaagacc ttaggcctgg cgaccttatg tattggtggc ggtcaaggta tcgccatggt
     1261 gatcgaacgc ctgaactgat gaggatctag gaggaaagca aaatgaaact gagcaccaag
     1321 ctgtgctggt gtggcatcaa gggtcgcctg cgcccacaaa agcagcaaca gctgcacaac
     1381 acgaacctgc aaatgaccga gctgaaaaag cagaagacgg ccgagcaaaa gacccgcccg
     1441 cagaacgttg gcatcaaggg catccagatt tatatcccga cgcagtgtgt caaccaatct
     1501 gagctggaga aattcgatgg cgtcagccag ggtaagtaca ccatcggcct gggccagacc
     1561 aacatgagct tcgtgaacga ccgtgaggac atctattcta tgagcctgac ggtgctgtct
     1621 aagctgatca agagctacaa catcgacacg aataagatcg gtcgtctgga ggtgggtacg
     1681 gagacgctga ttgacaagag caaaagcgtg aagtctgtct taatgcagct gttcggcgag
     1741 aacacggatg tcgagggtat cgacaccctg aacgcgtgtt acggcggcac caacgcactg
     1801 ttcaatagcc tgaactggat tgagagcaac gcctgggatg gccgcgatgc gatcgtcgtg
     1861 tgcggcgata tcgccatcta tgacaagggt gcggcacgtc cgaccggcgg tgcaggcacc
     1921 gttgcgatgt ggattggccc ggacgcacca attgtcttcg attctgtccg cgcgtcttac
     1981 atggagcacg cctacgactt ttacaagccg gacttcacga gcgaataccc gtacgtggac
     2041 ggccacttct ctctgacctg ctatgtgaag gcgctggacc aggtttataa gtcttatagc
     2101 aaaaaggcga tttctaaggg cctggtcagc gacccggcag gcagcgacgc cctgaacgtg
     2161 ctgaagtatt tcgactacaa cgtgttccat gtcccgacct gcaaattagt gaccaaatct
     2221 tatggccgcc tgttatataa tgatttccgt gccaacccgc agctgttccc ggaggttgac
     2281 gccgagctgg cgacgcgtga ttacgacgag agcctgaccg acaagaacat cgagaagacc
     2341 ttcgtcaacg tcgcgaagcc gttccacaaa gagcgtgtgg cccaaagcct gatcgtcccg
     2401 accaacacgg gcaacatgta taccgcgtct gtctacgcgg cattcgcgag cctgctgaat
     2461 tacgtcggtt ctgacgacct gcagggcaag cgcgttggcc tgttcagcta cggtagcggc
     2521 ttagcggcca gcctgtatag ctgcaaaatt gtcggcgacg tccagcacat catcaaggag
     2581 ctggacatca ccaacaagct ggcgaagcgc atcaccgaga cgccgaaaga ttacgaggca
     2641 gcgatcgagt tacgcgagaa tgcgcatctg aagaagaact tcaagccgca aggtagcatc
     2701 gagcacctgc agagcggcgt ctactacctg acgaacattg acgacaagtt ccgccgttct
     2761 tatgacgtca aaaagtaact agtaggagga aaacatcatg gtgctgacga acaaaaccgt
     2821 cattagcggc agcaaggtga agtctctgag cagcgcccaa agctctagca gcggcccgtc
     2881 tagcagcagc gaggaggacg acagccgtga cattgagtct ctggacaaga agatccgccc
     2941 gctggaggag ttagaggccc tgctgagcag cggcaacacc aagcagctga agaacaagga
     3001 agttgcagcg ctggtgatcc acggtaagct gccactgtat gcgctggaaa agaaactggg
     3061 cgatacgacg cgtgcggtcg cggtgcgtcg caaagcctta agcatcttag cggaggcccc
     3121 ggtgttagcc agcgaccgcc tgccgtacaa gaactacgac tacgaccgcg tgtttggcgc
     3181 gtgctgcgag aatgtcattg gctacatgcc gttaccggtt ggtgtgatcg gcccgctggt
     3241 cattgatggc acgagctatc acattccaat ggcgaccacg gaaggttgct tagtcgccag
     3301 cgccatgcgt ggctgtaagg cgattaacgc cggcggtggc gcgacgaccg tgttaaccaa
     3361 ggatggtatg acgcgcggtc cggtcgtccg cttcccaacg ctgaagcgca gcggcgcgtg
     3421 taagatttgg ctggattctg aggagggcca aaacgcgatc aagaaagcct tcaactctac
     3481 gagccgtttc gcgcgtttac agcatatcca gacctgcctg gccggcgacc tgctgttcat
     3541 gcgcttccgc accaccacgg gcgatgcgat gggcatgaac atgatcagca agggcgtcga
     3601 atatagcctg aaacaaatgg tggaagaata tggctgggag gacatggagg ttgtctctgt
     3661 gagcggcaac tattgcaccg acaagaagcc ggcagccatt aactggattg agggtcgcgg
     3721 caaaagcgtc gtggcagaag cgaccatccc aggcgacgtg gtccgtaagg ttctgaagag
     3781 cgacgtcagc gccctggttg agttaaatat cgcgaaaaac ctggtcggca gcgcgatggc
     3841 gggcagcgtg ggtggcttta acgcacatgc agcgaatctg gttacggcgg ttttcttagc
     3901 cttaggtcag gacccagccc aaaatgtcga gagcagcaac tgcattacct taatgaaaga
     3961 ggttgacggt gacctgcgca tcagcgtttc tatgccgtct atcgaggtcg gcacgatcgg
     4021 cggcggcacc gttttagaac cgcaaggtgc gatgctggat ctgctgggcg tgcgcggccc
     4081 acatgcaacg gccccaggca ccaatgcccg ccaactggcc cgtatcgtgg cctgcgcggt
     4141 tctggcgggt gagctgagcc tgtgcgccgc attagccgcg ggccatttag ttcaatctca
     4201 catgacccac aaccgcaagc cggcagaacc aaccaagcca aataacctgg acgcaaccga
     4261 cattaaccgt ctgaaggatg gcagcgtcac gtgcattaaa agctgatgag gatcttttta
     4321 aggatctcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     4381 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     4441 cctttctgcg tttatagcga attggatctg gtttgacagc ttatcatcga ctgcacggtg
     4501 caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta
     4561 aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg
     4621 ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatccg
     4681 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc aggatctagg aggaaataac
     4741 catgtctctg ccattcctga cgtctgcgcc aggtaaggtg atcatcttcg gcgagcactc
     4801 tgcggtgtac aataagccgg ccgtcgccgc ctctgtgtct gcgttacgca cctacctgct
     4861 gatcagcgaa tcttctgcac cggacacgat cgagctggac tttccggaca tcagcttcaa
     4921 ccacaagtgg agcatcaacg acttcaacgc gatcacggag gaccaggtga acagccaaaa
     4981 gctggccaaa gcccagcaag caaccgacgg tctgtctcag gagctggtgt ctctgctgga
     5041 cccgctgtta gcgcagttaa gcgagagctt ccattaccac gccgcgttct gcttcctgta
     5101 catgttcgtt tgcctgtgcc cgcacgcaaa gaacatcaag ttcagcctga agagcacgct
     5161 gccgattggc gcaggcttag gctctagcgc atctatcagc gtgagcctgg cgctggcgat
     5221 ggcctatctg ggtggcctga ttggcagcaa cgacctggag aaactgagcg aaaacgacaa
     5281 gcacatcgtg aaccagtggg cctttatcgg cgagaagtgc attcatggca ccccgagcgg
     5341 cattgacaac gcagttgcca cgtatggcaa cgccctgctg ttcgagaaag acagccacaa
     5401 cggcacgatc aacacgaaca acttcaagtt cctggacgac ttcccggcga tcccgatgat
     5461 tctgacctac acccgtatcc cacgcagcac caaggattta gtcgcccgcg tgcgtgtttt
     5521 agtcaccgaa aagttcccgg aggtgatgaa gccgatcctg gacgcgatgg gcgagtgcgc
     5581 gctgcagggt ctggagatca tgaccaagct gagcaagtgc aagggcaccg acgatgaggc
     5641 ggtggagacc aacaatgagc tgtacgagca gctgctggag ctgatccgta tcaatcacgg
     5701 cctgctggtc tctatcggtg tgtctcaccc gggcctggaa ctgatcaaaa acctgagcga
     5761 cgacctgcgc attggctcta cgaaattaac gggtgcaggt ggcggtggct gctctttaac
     5821 gctgctgcgc agtgacatta cgcaggagca aatcgacagc ttcaagaaga agctgcagga
     5881 cgacttcagc tacgagacgt tcgagacgga cctgggcggc acgggctgtt gcctgctgag
     5941 cgccaaaaat ctgaacaagg acctgaagat caaaagcctg gtgttccagc tgttcgaaaa
     6001 caagacgacc acgaagcagc agatcgacga cctgttactg ccgggtaaca ccaatctgcc
     6061 gtggacgtct tgaggatcta ggagggagat catatgagcg aattacgtgc attcagcgcg
     6121 ccaggtaagg cactgctggc cggtggctac ctggtgttag acaccaagta cgaggcgttc
     6181 gtcgtcggct tatctgcccg tatgcatgca gttgcccacc cgtatggtag cctgcagggc
     6241 tctgacaagt tcgaagtgcg tgtgaagagc aagcagttca aggacggcga gtggctgtac
     6301 cacattagcc caaagagcgg cttcatcccg gttagcattg gtggcagcaa gaacccattt
     6361 atcgagaagg tcattgccaa cgtcttcagc tacttcaagc cgaatatgga cgattactgc
     6421 aaccgcaacc tgttcgtcat cgacattttc agcgacgacg cgtaccacag ccaagaggac
     6481 tctgttacgg agcatcgtgg taaccgccgc ctgagcttcc acagccatcg cattgaggag
     6541 gtgccgaaga cgggtctggg ttctagcgcc ggtttagtta ccgtcttaac gacggcgtta
     6601 gcgagcttct tcgtgagcga cctggagaac aacgtggaca agtaccgcga agtgattcat
     6661 aacctggcgc aggtggcaca ttgtcaggcc caaggtaaga ttggctctgg ttttgatgtg
     6721 gcagcggccg cctatggctc tatccgctat cgccgctttc cgccggccct gatcagcaat
     6781 ctgccggaca tcggctctgc gacgtatggt agcaaactgg cgcatctggt ggacgaggag
     6841 gactggaaca tcaccattaa gtctaatcac ctgccgagcg gcttaacgtt atggatgggc
     6901 gatatcaaga acggcagcga aacggttaag ctggtgcaga aagtgaaaaa ctggtacgac
     6961 agccacatgc cggaaagcct gaagatttac acggagctgg accacgccaa tagccgtttc
     7021 atggatggtc tgagcaagct ggaccgcctg cacgaaaccc acgacgacta cagcgaccaa
     7081 atcttcgaga gcctggagcg caatgactgc acctgccaga agtacccgga gatcacggag
     7141 gtccgcgatg ccgtggcaac gattcgccgt agcttccgca aaattacgaa ggagagcggc
     7201 gcggatatcg aaccaccggt ccagacgtct ctgctggacg actgtcaaac cttaaagggc
     7261 gtgttaacgt gcctgattcc gggcgcgggt ggttacgacg ccattgccgt catcacgaaa
     7321 caggacgtcg atctgcgcgc acaaacggcc aacgacaaac gtttcagcaa agtccaatgg
     7381 ctggatgtta cgcaggccga ctggggtgtt cgcaaggaga aggacccgga aacgtatctg
     7441 gataagtgag gatctaggag gattatgaga tgaccgttta cacagcatcc gttaccgcac
     7501 ccgtcaacat cgcaaccctt aagtattggg ggaaaaggga cacgaagttg aatctgccca
     7561 ccaattcgtc catatcagtg actttatcgc aagatgacct cagaacgttg acctctgcgg
     7621 ctactgcacc tgagtttgaa cgcgacactt tgtggttaaa tggagaacca cacagcatcg
     7681 acaatgaaag aactcaaaat tgtctgcgcg acctacgcca attaagaaag gaaatggaat
     7741 cgaaggacgc ctcattgccc acattatctc aatggaaact ccacattgtc tccgaaaata
     7801 actttcctac agcagctggt ttagcttcct ccgctgctgg ctttgctgca ttggtctctg
     7861 caattgctaa gttataccaa ttaccacagt caacttcaga aatatctaga atagcaagaa
     7921 aggggtctgg ttcagcttgt agatcgttgt ttggcggata cgtggcctgg gaaatgggaa
     7981 aagctgaaga tggtcatgat tccatggcag tacaaatcgc agacagctct gactggcctc
     8041 agatgaaagc ttgtgtccta gttgtcagcg atattaaaaa ggatgtgagt tccactcagg
     8101 gtatgcaatt gaccgtggca acctccgaac tatttaaaga aagaattgaa catgtcgtac
     8161 caaagagatt tgaagtcatg cgtaaagcca ttgttgaaaa agatttcgcc acctttgcaa
     8221 aggaaacaat gatggattcc aactctttcc atgccacatg tttggactct ttccctccaa
     8281 tattctacat gaatgacact tccaagcgta tcatcagttg gtgccacacc attaatcagt
     8341 tttacggaga aacaatcgtt gcatacacgt ttgatgcagg tccaaatgct gtgttgtact
     8401 acttagctga aaatgagtcg aaactctttg catttatcta taaattgttt ggctctgttc
     8461 ctggatggga caagaaattt actactgagc agcttgaggc tttcaaccat caatttgaat
     8521 catctaactt tactgcacgt gaattggatc ttgagttgca aaaggatgtt gccagagtga
     8581 ttttaactca agtcggttca ggcccacaag aaacaaacga atctttgatt gacgcaaaga
     8641 ctggtctacc aaaggaataa ggatctagga ggtaatgata atgcaaacgg aacacgtcat
     8701 tttattgaat gcacagggag ttcccacggg tacgctggaa aagtatgccg cacacacggc
     8761 agacacccgc ttacatctcg cgttctccag ttggctgttt aatgccaaag gacaattatt
     8821 agttacccgc cgcgcactga gcaaaaaagc atggcctggc gtgtggacta actcggtttg
     8881 tgggcaccca caactgggag aaagcaacga agacgcagtg atccgccgtt gccgttatga
     8941 gcttggcgtg gaaattacgc ctcctgaatc tatctatcct gactttcgct accgcgccac
     9001 cgatccgagt ggcattgtgg aaaatgaagt gtgtccggta tttgccgcac gcaccactag
     9061 tgcgttacag atcaatgatg atgaagtgat ggattatcaa tggtgtgatt tagcagatgt
     9121 attacacggt attgatgcca cgccgtgggc gttcagtccg tggatggtga tgcaggcgac
     9181 aaatcgcgaa gccagaaaac gattatctgc atttacccag cttaaataag gatctaggag
     9241 gttactcata tggactttcc gcagcaactc gaagcctgcg ttaagcaggc caaccaggcg
     9301 ctgagccgtt ttatcgcccc actgcccttt cagaacactc ccgtggtcga aaccatgcag
     9361 tatggcgcat tattaggtgg taagcgcctg cgacctttcc tggtttatgc caccggtcat
     9421 atgttcggcg ttagcacaaa cacgctggac gcacccgctg ccgccgttga gtgtatccac
     9481 gcttactcat taattcatga tgatttaccg gcaatggatg atgacgatct gcgtcgcggt
     9541 ttgccaacct gccatgtgaa gtttggcgaa gcaaacgcga ttctcgctgg cgacgcttta
     9601 caaacgctgg cgttctcgat tttaagcgat gccgatatgc cggaagtgtc ggaccgcgac
     9661 agaatttcga tgatttctga actggcgagc gccagtggta ttgccggaat gtgcggtggt
     9721 caggcattag atttagacgc ggaaggcaaa cacgtacctc tggacgcgct tgagcgtatt
     9781 catcgtcata aaaccggcgc attgattcgc gccgccgttc gccttggtgc attaagcgcc
     9841 ggagataaag gacgtcgtgc tctgccggta ctcgacaagt atgcagagag catcggcctt
     9901 gccttccagg ttcaggatga catcctggat gtggtgggag atactgcaac gttgggaaaa
     9961 cgccagggtg ccgaccagca acttggtaaa agtacctacc ctgcacttct gggccttgag
    10021 caagcccgga agaaagcccg ggatctgatc gacgatgccc gtcagtcgct gaaacaactg
    10081 gctgaacagt cactcgatac ctcggcactg gaagcgctag cggactacat catccagcgt
    10141 aataaataat taggatccaa actcgagtaa ggatctccag gcatcaaata aaacgaaagg
    10201 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
    10261 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatacctag ggatatattc
    10321 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
    10381 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
    10441 agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
    10501 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10561 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
    10621 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
    10681 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
    10741 taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac
    10801 tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa
    10861 ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag
    10921 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
    10981 ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct
    11041 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
    11101 agttgttact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
    11161 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
    11221 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
    11281 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
    11341 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
    11401 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
    11461 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
    11521 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
    11581 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
    11641 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
    11701 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
    11761 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
    11821 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
    11881 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
    11941 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
    12001 tctcattttc gccagatatc gacgtcggtg cctaatgagt gagctaactt acattaattg
    12061 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    12121 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
    12181 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
    12241 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
    12301 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca
    12361 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
    12421 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
    12481 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
    12541 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
    12601 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
    12661 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
    12721 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
    12781 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
    12841 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
    12901 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
    12961 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
    13021 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
    13081 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
    13141 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
    13201 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc
    13261 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa
    13321 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
    13381 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
    13441 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
    13501 tccggcgtag aggatcgaga tcgtttaggc accccaggc
//
