LOCUS       sequence-357461-        9934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of WT golgin-160.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Hicks SW, Machamer CE
  TITLE     The NH2-terminal domain of Golgin-160 contains both Golgi and 
            nuclear targeting information.
  JOURNAL   J Biol Chem. 2002 Sep 27. 277(39):35833-9.
  PUBMED    12130652
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Sep 27. 277(39):35833-9."
COMMENT     Sequence Label: pCDNA/golgin-160/myc
FEATURES             Location/Qualifiers
     source          1..9934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        122..501
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        502..705
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     656..676
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     750..769
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        750..768
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5325..5354
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5370..5387
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5410..5427)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5416..5640
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5686..6114
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5773..5792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5983..6004
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6123..6143)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6128..6457
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6308..6443
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6370..6389
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6524..7318
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6578..6597)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7188..7207
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7494..7615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7531..7550)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7585..7604
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7664..7680)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7664..7680)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7677..7699)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7688..7704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7712..7718,7719..7736,7737..7742))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7757..7778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7895..7912)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8066..8654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8146..8165)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8825..9616,9617..9685))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9448..9467
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9686..9790)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9865..9884)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
       61 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      121 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      181 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      241 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      301 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      361 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      421 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      481 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      541 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      601 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      661 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
      721 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
      781 ggctagttaa gcttggtacc gagctcggat ctcgagctat ggacggcgcg tcggccgagc
      841 aagatggcct ccaggaggac agatcccaca gtggcccctc gtctctcccc gaggccccac
      901 tgaagccccc gggcccactg gtgccacctg accagcagga caaagtccag tgtgccgagg
      961 taaacagagc atccacggaa ggggaaagcc cggatggacc tggccaggga ggcctctgtc
     1021 agaacgggcc aacgccaccc ttcccagacc ctccgtcgtc tctcgatccc accacaagcc
     1081 cagtgggccc tgatgcctct ccaggtgtgg ctggtttcca tgacaaccta aggaagtctc
     1141 agggaactag tgctgagggc agtgttagaa aagaagcttt gcagtctctc agactcagtc
     1201 ttcctatgca agaaacgcaa ctgtgctcta cagattctcc cctgcccctg gagaaggagg
     1261 agcaggtccg acttcaggct cggaagtggc tgaaagagca gctcaaacag tacagggtga
     1321 agcgccagca ggagaggtcc agtcaacctg caaccaaaac gagacttttt agcacgcttg
     1381 atcctgagct catgttaaac ccagaaaact taccaagggc cagtaccctg gctatgacaa
     1441 aagaatattc cttcctgcgc accagtgtcc ctcgggggcc taaggtgggc agcctggggc
     1501 ttccggcaca tcctagggag aaaaaaactt ccaaatcaag caaaatccgg tctctggccg
     1561 attacagaac tgaagattca aatgcgggga attctggggg aaatgtcccg gctcccgatt
     1621 ctaccaaggg ttccctgaag cagaacagaa gcagtgcggc gtccgttgtg tctgagatca
     1681 gcctgtcccc cgacactgac gaccgtctgg agaacacctc cctggctgga gacagcgtgt
     1741 ctgaggtgga tggaaatgac agcgacagct catcgtacag cagcgcctcc acccgaggga
     1801 cctatggcat tctgtcgaag acagtgggca cgcaggacac cccctatatg gtcaacggcc
     1861 aggagattcc tgcggatacc ctgggccagt tcccctccat taaggacgtc ctccaggccg
     1921 cagccgctga gcaccaagac caggggcagg aggtcaacgg ggaggtgcgg agtcggagag
     1981 acagcatctg cagcagcgtg tccttggaga gctctgcagc agaaacacag gaggagatgc
     2041 tgcaggtgct caaagagaaa atgcgactcg aaggacagct ggaagccttg tcactggagg
     2101 cgagtcaggc acttaaagag aaggctgagc tgcaggccca gctggccgcc ctcagcacga
     2161 agctgcaggc gcaggtggag tgcagccaca gcagccagca gcggcaggat tcgctgagct
     2221 cggaggtgga caccctgaag cagtcgtgct gggacctgga gcgagccatg actgacctgc
     2281 agaacatgct ggaggcaaaa aatgccagcc tggcgtcgtc caacaacgac ttgcaggtgg
     2341 ccgaggagca gtaccagagg cttatggcca aggtagagga catgcagagg agcatgctca
     2401 gcaaggacaa cacagtgcac gacctgcgac agcagatgac agccttgcag agccagcttc
     2461 agcaggtgca gctggagcgg acgacgctga ccagcaagct gaaggcgtcg caggcggaga
     2521 tctcgtccct acagagtgtc cggcagtggt accagcagca gctcgccctg gcacaggagg
     2581 cccgcgtcag gctgcagggt gagatggccc acatccaggt tggacagatg acccaggcag
     2641 gtctcctgga gcacctgaaa ctcgagaatg tgtccctgtc ccagcagctg acggaaactc
     2701 agcacaggtc catgaaggag aaggggcgca tcgcggcaca gctgcagggc attgaggctg
     2761 acatgttgga tcaggaagca gccttcatgc agattcagga ggcaaagacg atggtggagg
     2821 aggaccttca gaggaggctg gaagagtttg aaggtgagag ggagcggctg cagaggatgg
     2881 cggactcggc ggcatccctg gagcagcagc tggagcaggt gaagttgact ttactccagc
     2941 gagaccagca gcttgaggct ttgcagcagg agcacctgga cctgatgaaa cagctcacct
     3001 tgactcagga ggctctgcag agcagggagc agtccctcga tgccctgcag acacgctacg
     3061 atgagctgca ggccaggctg ggggagctgc agggcgaggc cgcctccagg gaggacacga
     3121 tctgcctcct gcagaacgag aagatcatct tggaggcggc tttgcaggcg gccaagagtg
     3181 gcaaggagga gcttgacaga ggagcaagac gcttggaaga aggtaccgag gaaacgtcgg
     3241 aaactttaga gaagttaaga gaagaattag ctatcaaatc cggccaggtg gaacacctgc
     3301 agcaggagac tgctgctctg aaaaagcaaa tgcaaaaaat aaaggaacag tttctccaac
     3361 aaaaggtgat ggtggaggcc taccggcgcg acgccacctc caaagaccag ctcatcagtg
     3421 agctgaaagc caccaggaag aggctggact cggagctgaa ggagctgcgg caggagctga
     3481 tgcaagtgca cggggagaag cggactgccg aggcggagct ctcgcgcctg cacagagagg
     3541 tggcccaggt ccgtcagcac atggcggacc ttgaagggca tctccagtcg gcgcagaagg
     3601 agcgagacga ggtggaaaca cacttgcagt cgttgcagtt cgataaggag cagatggtcg
     3661 cggtcacaga ggccaatgag gcgctgaaga aacaaatcga agagttgcag caagaggccc
     3721 ggaaggccat cacggaacag aagcagaaga tgaggcggct gggctcagac ttgaccagcg
     3781 cccagaagga gatgaagacc aaacataagg cctacgagaa cgccgtgggc atcctcagcc
     3841 gccgcctgca ggaagccctc gcggccaggg aggctgcgga cgcggagcta ggccagctcc
     3901 gagcccaggg tggcagcagt gacagcagcc tggctctaca tgaaaggatc caggccctgg
     3961 aggcggagct gcaggctgtc agtcatagca agacgctgct ggaaaaggaa ctgcaggagg
     4021 tcatagcgct gaccagccag gagctggagg agtcccggga gaaggtgctg gagctggagg
     4081 acgagcttca agaatccaga ggctttagga agaagataaa acgccttgag gagtcaaaca
     4141 agaagttggc tcttgaatta gagcacgaga aagggaagct tacgggcctc ggtcagtcca
     4201 acgcagctct gcgggaacac aacagcatcc tagaaacagc tttggccaag agggaggcag
     4261 acctagtcca gttgaacctt caggtgcagg cagttttgca gcgcaaagaa gaggaggatc
     4321 gccagatgaa gcatcttgtc caggccctgc aggcctcact agagaaggag aaggagaagg
     4381 tgaacagcct caaggagcag gtggctgctg ccaaggtgga agccgggcat aaccgccgcc
     4441 acttcaaggc ggcctccttg gagctgagtg aggtgaagaa ggagctgcag gccaaggaac
     4501 acctggtgca gaagctgcag gccgaggccg acgaccttca gattcgggag gggaaacatt
     4561 cccaggagat agcacagttc caagcagagc tggccgaggc ccgggcacag ctccagctcc
     4621 tgcagaagca gctggacgag cagctcagca aacagcccgc gggaaaccaa gagatggaaa
     4681 atctcaaatg ggaggtggat cagaaagaaa gagaaatcca gtccttgaag cagcagctgg
     4741 acttgacgga gcagcagggc tggaaggaac tggaagggct acagcagctg ctgcagaacg
     4801 tcaagtctga gttggagatg gcccaggaag acctgtccat gacccagaag gataaattta
     4861 tgctccaggc aaaagtgtcg gagctgaaga acaacatgaa gaccctgctc cagcagaacc
     4921 agcagctcaa gctggaccta cgccgcggcg cggccaagac gagaaaggag ccgaaaggcg
     4981 aggccagctc ttccaaccct gccacgccca tcaagatccc ggactgccca gttcccgcct
     5041 cgctgctgga ggagctgctg agaccaccgc ccgccgtgag caaggagccc ctcaagaacc
     5101 tgaacagctg cctccagcag ctcaagcagg agatggacag cctgcggcgc cagatggagg
     5161 agcacgccct gacggtgcac gagtctctgt cctcgtggac gccgctggag ccagccactg
     5221 ccagccctgt gcccccgggg ggtcacgccg gcccacgcgg cgacccacag agacacagtc
     5281 agagcagggc ttccaaagaa gggcctctag agggcccgcg gttcgaacaa aaactcatct
     5341 cagaagagga tctgaatatg cataccggtc atcatcacca tcaccattga gtttaaaccc
     5401 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5461 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5521 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     5581 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     5641 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     5701 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5761 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5821 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5881 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5941 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6001 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6061 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6121 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6181 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6361 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6421 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     6481 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     6541 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     6601 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     6661 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     6721 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     6781 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6841 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6901 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6961 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     7021 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     7081 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     7141 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     7201 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     7261 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     7321 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     7381 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7441 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     7501 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7561 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7621 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     7681 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7741 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7801 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7861 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8041 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8581 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8701 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8761 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8821 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8881 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8941 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9001 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9061 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9121 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9181 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9241 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9301 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9361 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9421 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9481 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9541 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9601 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9661 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9721 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9781 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     9841 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     9901 tctgctccct gcttgtgtgt tggaggtcgc tgag
//
