LOCUS       sequence-357464-       11194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To convert human skin fibroblasts into induced basal forebrain 
            cholinergic neurons (hiBFCNs) in combination with ASCL1, Sox11, FGF2
            and two small molecules, forskolin and LDN-193189.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Ma S, Zang T, Liu ML, Zhang CL
  TITLE     Aging-relevant human basal forebrain cholinergic neurons as a cell 
            model for Alzheimer's disease.
  JOURNAL   Mol Neurodegener. 2020 Oct 21;15(1):61. doi: 
            10.1186/s13024-020-00411-6.
  PUBMED    33087140
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurodegener."; date: "2020-10-21"; volume: "15(1):61. doi"; pages: 
            " 10.1186/s13024-020-00411-6"
COMMENT     Sequence Label: pCSC-LHX8-IRES-GBX1
FEATURES             Location/Qualifiers
     source          1..11194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(4349..4366)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4576..4595
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6001..6589
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6054..6074)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6472..6483)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6996..7176
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7202..7219)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7208..7432
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7478..7906
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7565..7584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7775..7796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7915..7935)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7920..8249
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8100..8235
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8162..8181
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8297..8344
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8363..8737
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8867..8988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8904..8923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8958..8977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9037..9053)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9037..9053)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9050..9072)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9061..9077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9085..9091,9092..9109,9110..9115))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9130..9151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9268..9285)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9439..10027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9519..9538)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10198..10989,10990..11058))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10821..10840
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11059..11163)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agaggatccg
     3121 ccaccatgca gattctgagc aggtgtcagg ggctcatgtc agaggagtgc gggcggacta
     3181 cagccctggc ggccgggagg actcgcaaag gcgccgggga agagggactg gtgagccccg
     3241 agggagcggg ggacgaggac tcgtgctcct cctcggcccc gctgtccccg tcgtcctcgc
     3301 cccggtccat ggcctcgggc tccggctgcc ctcctggcaa gtgtgtgtgc aacagttgcg
     3361 gcctggagat cgtggacaaa taccttctca aggtgaatga cctatgctgg catgtccggt
     3421 gtctctcctg cagtgtttgc agaacctccc taggaaggca caccagctgt tatattaaag
     3481 acaaagacat tttctgcaaa cttgattatt tcagaaggta tggaactcgc tgctctcgat
     3541 gtgggagaca catccattct actgactggg tccggagagc caaggggaat gtctatcact
     3601 tggcatgctt tgcctgcttt tcctgcaaaa ggcaactttc cacaggagag gagtttgctt
     3661 tggtggaaga gaaagtcctc tgcagagtac attatgactg catgctggat aatttaaaaa
     3721 gagaagtaga aaatgggaat gggattagtg tggaaggtgc cctcctcaca gagcaagatg
     3781 ttaaccatcc aaaaccagca aaaagagctc ggaccagctt tacagcagat cagcttcagg
     3841 ttatgcaagc acaatttgct caggacaaca acccagatgc acagacactc cagaaattgg
     3901 cagaaaggac aggcttgagc agacgtgtga tacaggtgtg gtttcagaat tgtagagcac
     3961 gccacaagaa acacgtcagt cctaatcact catcctccac cccagtcaca gcagccccac
     4021 cctccaggct gtctccaccc atgttagaag aaatggctta ttctgcctac gtgccccaag
     4081 atggaacgat gttaactgcg ctgcatagtt atatggatgc tcattcacca acaactcttg
     4141 gactccagcc cttgttaccc cattcaatga cacaactgcc aataagtcat acctaactcg
     4201 actaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     4261 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     4321 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     4381 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     4441 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     4501 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     4561 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     4621 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     4681 tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     4741 aaacacgata atgctagagg atccgccacc atgcagcggg ccggaggcgg tagcgcccct
     4801 gggggcaacg gcgggggcgg cggcgggggc ccgggcactg ccttctccat cgactcccta
     4861 atcgggccgc cgccgccgcg ctccggccac ttgctgtaca ccggctaccc catgttcatg
     4921 ccctaccggc cgctcgtgct gccgcaggcg ctggcccctg cgccgctgcc cgctggcctc
     4981 ccgcccctcg ccccgctagc ctctttcgcc ggccgcctta ccaacacctt ctgcgcgggg
     5041 ctgggtcagg ctgtgccctc gatggtggcg ctgaccaccg cgctgcccag cttcgcggag
     5101 ccgcccgacg ctttctacgg gccccaggag ctcgccgccg ccgctgccgc cgccgccgcc
     5161 actgccgccc gaaacaaccc cgagccaggc ggccgacgcc cagagggtgg gctggaagct
     5221 gatgagctgc tgccggcccg ggagaaagtg gcagagcccc caccacctcc gcctccgcac
     5281 ttctcagaga cttttccaag tctgcccgca gaggggaagg tgtacagctc agatgaggag
     5341 aagctggagg catcagcagg agacccagca ggcagcgaac aggaggaaga gggctcaggc
     5401 ggtgacagcg aggatgacgg tttcctggac agttctgcag ggggcccagg ggctcttctg
     5461 ggacctaaac cgaagctaaa gggaagcctg gggactggag ctgaggaggg ggcaccggtg
     5521 acagcagggg tcacagctcc tggggggaaa agccgacggc gccgcacagc atttaccagc
     5581 gagcagcttt tggaattgga gaaggaattt cattgcaaga aatacctgag cttgacagag
     5641 cgctctcaga tcgcccacgc cctcaagctc agtgaggtgc aggtcaagat ctggtttcag
     5701 aatcgacggg ccaagtggaa gcgcatcaaa gctggcaatg tgagcagccg ttctggggag
     5761 cccgtaagaa accccaagat tgttgtcccc atacctgtgc atgtcaacag gtttgctgtg
     5821 cggagccagc accaacaaat ggagcagggg gccaggccct agctcgaggg atcaagctta
     5881 tcgaaacctg gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     5941 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcagac
     6001 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6061 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6121 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6181 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6241 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6301 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6361 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     6421 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6481 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6541 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
     6601 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6661 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     6721 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     6781 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     6841 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     6901 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     6961 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     7021 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7081 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7141 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc cgctttaaac ccgctgatca
     7201 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7261 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7321 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7381 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     7441 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     7501 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7561 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7621 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7681 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7741 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7801 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7861 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7921 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7981 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8041 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8101 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8161 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     8221 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     8281 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     8341 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     8401 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     8461 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     8521 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     8581 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     8641 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     8701 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8761 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8821 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     9001 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9061 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9121 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9181 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9241 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9301 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9361 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9421 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9481 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9541 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9601 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9661 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9721 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9781 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9841 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9901 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9961 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10021 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10081 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10141 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10201 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10261 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10321 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10381 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10441 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10501 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10561 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10621 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10681 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10741 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10801 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10861 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10921 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10981 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11041 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11101 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11161 gcgcacattt ccccgaaaag tgccacctga cgtc
//
