LOCUS       sequence-357466-       10970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To convert human skin fibroblasts into induced basal forebrain 
            cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 
            and two small molecules, forskolin and LDN-193189.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10970)
  AUTHORS   Ma S, Zang T, Liu ML, Zhang CL
  TITLE     Aging-relevant human basal forebrain cholinergic neurons as a cell 
            model for Alzheimer's disease.
  JOURNAL   Mol Neurodegener. 2020 Oct 21;15(1):61. doi: 
            10.1186/s13024-020-00411-6.
  PUBMED    33087140
REFERENCE   2  (bases 1 to 10970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurodegener."; date: "2020-10-21"; volume: "15(1):61. doi"; pages: 
            " 10.1186/s13024-020-00411-6"
COMMENT     Sequence Label: pCSC-ASCL1-IRES-Sox11
FEATURES             Location/Qualifiers
     source          1..10970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3126..3135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4017..4034)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4244..4263
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      4445..4454
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5777..6365
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5830..5850)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6248..6259)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6772..6952
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6978..6995)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6984..7208
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7254..7682
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7341..7360)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7551..7572
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7691..7711)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7696..8025
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7876..8011
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7938..7957
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8073..8120
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8139..8513
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8643..8764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8680..8699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8734..8753
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8813..8829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8813..8829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8826..8848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8837..8853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8861..8867,8868..8885,8886..8891))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8906..8927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9044..9061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9215..9803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9295..9314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9974..10765,10766..10834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10597..10616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10835..10939)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agaggatcca
     3121 ccggtgccac catggaaagc tctgccaaga tggagagcgg cggcgccggc cagcagcccc
     3181 agccgcagcc ccagcagccc ttcctgccgc ccgcagcctg tttctttgcc acggccgcag
     3241 ccgcggcggc cgcagccgcc gcagcggcag cgcagagcgc gcagcagcag cagcagcagc
     3301 agcagcagca gcagcaggcg ccgcagctga gaccggcggc cgacggccag ccctcagggg
     3361 gcggtcacaa gtcagcgccc aagcaagtca agcgacagcg ctcgtcttcg cccgaactga
     3421 tgcgctgcaa acgccggctc aacttcagcg gctttggcta cagcctgccg cagcagcagc
     3481 cggccgccgt ggcgcgccgc aacgagcgcg agcgcaaccg cgtcaagttg gtcaacctgg
     3541 gctttgccac ccttcgggag cacgtcccca acggcgcggc caacaagaag atgagtaagg
     3601 tggagacact gcgctcggcg gtcgagtaca tccgcgcgct gcagcagctg ctggacgagc
     3661 atgacgcggt gagcgccgcc ttccaggcag gcgtcctgtc gcccaccatc tcccccaact
     3721 actccaacga cttgaactcc atggccggct cgccggtctc atcctactcg tcggacgagg
     3781 gctcttacga cccgctcagc cccgaggagc aggagcttct cgacttcacc aactggttcg
     3841 gatcctagat gaattccgcc cccccccccc taacgttact ggccgaagcc gcttggaata
     3901 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     3961 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     4021 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     4081 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     4141 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     4201 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     4261 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4321 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
     4381 aaccacgggg acgtggtttt cctttgaaaa acacgataat gctagcgcta ccggactcag
     4441 atccgccacc atggtgcagc aggccgagag ctcggaagcc gagagcaacc tgccccggga
     4501 cgcgctggac accgaggagg gcgagttcat ggcgtgcagc ccggtggccc tggacgagag
     4561 cgacccggac tggtgcaaga cggcgtcggg ccacatcaaa cggcccatga acgccttcat
     4621 ggtgtggtcc aagatcgagc gcaggaagat catggagcag tcgcccgaca tgcacaacgc
     4681 cgagatctcc aagaggctgg gcaagcgctg gaagatgctg aaggacagcg agaagatccc
     4741 gttcatcagg gaggcggagc gcctgcgcct caagcacatg gctgattatc ccgactacaa
     4801 gtaccggccg cgcaaaaagc ccaagacgga cccagcggcc aagcccagcg cgggccagag
     4861 ccccgacaag agcgcggcgg gcgccaaggc agccaagggc cccggcaaga agtgcgccaa
     4921 gctcaaggcg cctgcgggca aggcgggcgc gggcaaggcg gcgcagccgg gggactgcgc
     4981 cgcgggcaag gcagccaagt gcgtcttcct ggacgacgac gatgaagacg acgacgaaga
     5041 tgacgagctg cagctacggc ccaagccgga cgctgacgac gacgacgacg agcccgcgca
     5101 ctcgcacctg ctgccgccgc cgacgcagca gcaaccccct cagctgctga ggcgctacag
     5161 cgtggccaag gtccccgcca gccccacgct cagcagtgcc gccgagtccc ccgagggcgc
     5221 gagcctgtac gacgaagtgc gcgcgggcgg ccggctctac tacagcttca agaacatcac
     5281 caagcagcag cctccgcccg cgcctcccgc gctgtcgccc gcgtcctccc gctgcgtgtc
     5341 cacctcctca tccagcggca gcagcagcgg cagcggcgcc gaggatgcag acgacctcat
     5401 gttcgacctg agcttgaatt tctcccaggg cgcgcacagc gcctgcgagc agccactggg
     5461 cgcgggagcg gcggggaacc tgtccctgtc gctggtggat aaggacctgg attccttcag
     5521 cgagggcagc ctgggttccc acttcgagtt ccccgactac tgcacgccgg agctgagcga
     5581 gatgatcgcg ggggactggc tggaggcgaa cttctccgac ctggtgttca cgtattgact
     5641 cgagggatca agcttatcga aacctggaaa aacatggagc aatcacaagt agcaatacag
     5701 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     5761 cagtcacacc tcagacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     5821 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     5881 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     5941 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6001 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6061 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6121 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     6181 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6241 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6301 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6361 cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
     6421 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6481 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     6541 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     6601 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     6661 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     6721 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     6781 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6841 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6901 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggccgct
     6961 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     7021 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     7081 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     7141 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     7201 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     7261 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7321 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7381 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     7441 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     7501 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     7561 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     7621 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     7681 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7741 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7801 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7861 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7921 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     7981 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     8041 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     8101 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     8161 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     8221 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     8281 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     8341 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     8401 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     8461 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     8521 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     8581 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     8641 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8701 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8761 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     8821 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8881 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8941 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9001 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9061 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9121 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9181 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9241 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9301 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9421 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9541 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9601 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9661 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9721 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9781 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9841 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9901 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9961 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    10021 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10081 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10141 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10201 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10261 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10321 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10381 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10441 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10501 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10561 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10621 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10681 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10741 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10801 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10861 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10921 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
