LOCUS       sequence-357468-       11743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To convert human skin fibroblasts into induced basal forebrain 
            cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 
            and two small molecules, forskolin and LDN-193189.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Ma S, Zang T, Liu ML, Zhang CL
  TITLE     Aging-relevant human basal forebrain cholinergic neurons as a cell 
            model for Alzheimer's disease.
  JOURNAL   Mol Neurodegener. 2020 Oct 21;15(1):61. doi: 
            10.1186/s13024-020-00411-6.
  PUBMED    33087140
REFERENCE   2  (bases 1 to 11743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurodegener."; date: "2020-10-21"; volume: "15(1):61. doi"; pages: 
            " 10.1186/s13024-020-00411-6"
COMMENT     Sequence Label: pCSC-ASCL1-IRES-GFP-T2A-Sox11
FEATURES             Location/Qualifiers
     source          1..11743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3126..3135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3933..4394
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3989..4006)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4216..4235
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4399..4401,4402..4404,4405..5115)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4399..4401,4402..4404,4405..5115)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4444..4465)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4705..4724)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5052..5073
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5134..5187
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5212..5221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6550..7138
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6603..6623)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7021..7032)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7545..7725
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7751..7768)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7757..7981
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8027..8455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8114..8133)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8324..8345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8464..8484)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8469..8798
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8649..8784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8711..8730
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8846..8893
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8912..9286
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9416..9537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9453..9472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9507..9526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9586..9602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9586..9602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9599..9621)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9610..9626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9634..9640,9641..9658,9659..9664))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9679..9700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9817..9834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9988..10576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10068..10087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10747..11538,11539..11607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11370..11389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11608..11712)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agaggatcca
     3121 ccggtgccac catggaaagc tctgccaaga tggagagcgg cggcgccggc cagcagcccc
     3181 agccgcagcc ccagcagccc ttcctgccgc ccgcagcctg tttctttgcc acggccgcag
     3241 ccgcggcggc cgcagccgcc gcagcggcag cgcagagcgc gcagcagcag cagcagcagc
     3301 agcagcagca gcagcaggcg ccgcagctga gaccggcggc cgacggccag ccctcagggg
     3361 gcggtcacaa gtcagcgccc aagcaagtca agcgacagcg ctcgtcttcg cccgaactga
     3421 tgcgctgcaa acgccggctc aacttcagcg gctttggcta cagcctgccg cagcagcagc
     3481 cggccgccgt ggcgcgccgc aacgagcgcg agcgcaaccg cgtcaagttg gtcaacctgg
     3541 gctttgccac ccttcgggag cacgtcccca acggcgcggc caacaagaag atgagtaagg
     3601 tggagacact gcgctcggcg gtcgagtaca tccgcgcgct gcagcagctg ctggacgagc
     3661 atgacgcggt gagcgccgcc ttccaggcag gcgtcctgtc gcccaccatc tcccccaact
     3721 actccaacga cttgaactcc atggccggct cgccggtctc atcctactcg tcggacgagg
     3781 gctcttacga cccgctcagc cccgaggagc aggagcttct cgacttcacc aactggttct
     3841 gatatcgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     3901 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     3961 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     4021 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     4081 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     4141 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     4201 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     4261 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     4321 tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     4381 aaacacgatg ataataccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     4441 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     4501 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     4561 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     4621 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     4681 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4741 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4801 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     4861 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     4921 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4981 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     5041 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     5101 gacgagctgt acaagtccgg actcagatcc tacgagggga gaggaagtct tctgacctgc
     5161 ggagacgtcg aagagaatcc tggacccgct agcgctaccg gactcagatc cgccaccatg
     5221 gtgcagcagg ccgagagctc ggaagccgag agcaacctgc cccgggacgc gctggacacc
     5281 gaggagggcg agttcatggc gtgcagcccg gtggccctgg acgagagcga cccggactgg
     5341 tgcaagacgg cgtcgggcca catcaaacgg cccatgaacg ccttcatggt gtggtccaag
     5401 atcgagcgca ggaagatcat ggagcagtcg cccgacatgc acaacgccga gatctccaag
     5461 aggctgggca agcgctggaa gatgctgaag gacagcgaga agatcccgtt catcagggag
     5521 gcggagcgcc tgcgcctcaa gcacatggct gattatcccg actacaagta ccggccgcgc
     5581 aaaaagccca agacggaccc agcggccaag cccagcgcgg gccagagccc cgacaagagc
     5641 gcggcgggcg ccaaggcagc caagggcccc ggcaagaagt gcgccaagct caaggcgcct
     5701 gcgggcaagg cgggcgcggg caaggcggcg cagccggggg actgcgccgc gggcaaggca
     5761 gccaagtgcg tcttcctgga cgacgacgat gaagacgacg acgaagatga cgagctgcag
     5821 ctacggccca agccggacgc tgacgacgac gacgacgagc ccgcgcactc gcacctgctg
     5881 ccgccgccga cgcagcagca accccctcag ctgctgaggc gctacagcgt ggccaaggtc
     5941 cccgccagcc ccacgctcag cagtgccgcc gagtcccccg agggcgcgag cctgtacgac
     6001 gaagtgcgcg cgggcggccg gctctactac agcttcaaga acatcaccaa gcagcagcct
     6061 ccgcccgcgc ctcccgcgct gtcgcccgcg tcctcccgct gcgtgtccac ctcctcatcc
     6121 agcggcagca gcagcggcag cggcgccgag gatgcagacg acctcatgtt cgacctgagc
     6181 ttgaatttct cccagggcgc gcacagcgcc tgcgagcagc cactgggcgc gggagcggcg
     6241 gggaacctgt ccctgtcgct ggtggataag gacctggatt ccttcagcga gggcagcctg
     6301 ggttcccact tcgagttccc cgactactgc acgccggagc tgagcgagat gatcgcgggg
     6361 gactggctgg aggcgaactt ctccgacctg gtgttcacgt attgaagtac gctcgaggga
     6421 tcaagcttat cgaaacctgg aaaaacatgg agcaatcaca agtagcaata cagcagctac
     6481 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     6541 acctcagaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6601 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6661 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6721 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6781 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6841 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6901 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     6961 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7021 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7081 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     7141 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7201 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     7261 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     7321 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7381 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     7441 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     7501 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     7561 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7621 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7681 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc gctttaaacc
     7741 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7801 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7861 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7921 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     7981 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     8041 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8101 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8161 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8221 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     8281 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8341 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     8401 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     8461 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     8521 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     8581 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     8641 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8701 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     8761 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     8821 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     8881 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     8941 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     9001 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     9061 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     9121 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     9181 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     9241 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     9301 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     9361 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     9421 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9481 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9541 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     9601 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     9661 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     9721 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9781 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     9901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     9961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    10201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    10441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    10621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    10681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    10741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    10801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    10861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    10921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    10981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    11041 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    11161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    11221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    11281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    11341 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    11401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    11461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    11521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    11581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    11641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    11701 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
