LOCUS       sequence-357469-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient transfection plasmid expressing 
            Renilla-luciferase-beta-globin fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: pCMV-3XFLAG-renilla-luciferase-beta-globin-con...
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(90..114)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    148..770
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(347..366)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 ccccgctgca caggctgcct tccagaaggt ggtggctgga gtggccactg ccctggccca
       61 caagtatcac taaaagggtg ggcgcgccga cccagctttc ttgtacaaag tggttgatat
      121 cggtaccagt cgactctaga ggatcccggg tggcatccct gtgacccctc cccagtgcct
      181 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
      241 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
      301 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
      361 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
      421 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
      481 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
      541 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
      601 accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc
      661 cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt
      721 ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg
      781 ccagcttggc tgtggaatgt gtgtc
//
