LOCUS       sequence-357471-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient transfection plasmid expressing 
            Renilla-luciferase-beta-globin fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: pCMV-3XFLAG-renilla-luciferase-beta-globin-con...
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..99
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agctcgttta gtgaaccgtc agaattaacc atggactaca aagaccatga cggtgattat
       61 aaagatcatg acatcgatta caaggatgac gatgacaagc ttgcggccgc gaattcaact
      121 tcgaaagttt atgatccaga acaaaggaaa cggatgataa ctggtccgca gtggtgggcc
      181 agatgtaaac aaatgaatgt tcttgattca tttattaatt attatgattc agaaaaacat
      241 gcagaaaatg ctgttatttt tttacatggt aacgcggcct cttcttattt atggcgacat
      301 gttgtgccac atattgagcc agtagcgcgg tgtattatac cagaccttat tggtatgggc
      361 aaatcaggca aatctggtaa tggttcttat aggttacttg atcattacaa atatcttact
      421 gcatggtttg aacttcttaa tttaccaaag aagatcattt ttgtcggcca tgattggggt
      481 gcttgtttgg catttcatta tagctatgag catcaagata agatcaaagc aatagttcac
      541 gctgaaagtg tagtagatgt gattgaatca tgggatgaat ggcctgatat tgaagaagat
      601 attgcgttga tcaaatctga agaangagaa aaaatggttt tggagaataa cttcttcgt
//
