LOCUS       sequence-357472-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient transfection plasmid expressing 
            Renilla-luciferase-beta-globin fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: pCMV-3XFLAG-renilla-luciferase-beta-globin-con...
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(764..788)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggctattct gctcaacctt cctatcagaa aaaaagggga agcgattcta gggagcagtc
       61 tccatgactg tgtgtggagt gttgacaaga gttcggatat tttattctct actcagaatt
      121 gctgctcccc ctcactctgt tctgtgttgt catttcctct ttctttggta agcttttaat
      181 ttccagttgc attttactaa attaattaag ctggttattt acttcccatc ctgatatcag
      241 cttcccctcc tcctttcctc ccagtccttc tctctctcct ctctctttct ctaatccttt
      301 cctttccctc agttcatttc ttcttctttg atctacgttt gtttgtcttt ttaaatattg
      361 ccttgtaact tgctcagagg acaaggaaga tatgtccctg tttcttctca tagctctcaa
      421 gaatagtagc ataattggct tttatgccag ggtgacaggg gaagaatata ttttacatat
      481 aaattctgtt tgacatagga ttcttataat aatttgtcag tagtttaagg ttgcaaacaa
      541 atgtctttgt aaataagcct gcagtatctg gtatttttgc tctacagtta tgttgatggt
      601 tcttccatat tcccacagct cctgggcaat atgatcgtga ttgtgctggg ccaccacctg
      661 ggcaaggatt tcacccccgc tgcacaggct gccttccaga aggtggtggc tggagtggcc
      721 actgccctgg cccacaagta tcactaaaag ggtgggcgcg ccgacccagc tttcttgtac
      781 aaagtggttg atatcggtac cagtcgactc tagaggatcc cgggtggcat ccctgtgacc
      841 cct
//
