LOCUS       sequence-357474-       12900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression of Arch-based voltage indicator (QuasAr6a) and 
            membrane-localized calcium reporter (jRGECO1a-CAAX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12900)
  AUTHORS   Landau AT, Park P, Wong-Campos JD, Tian H, Cohen AE, Sabatini BL
  TITLE     Dendritic branch structure compartmentalizes voltage-dependent 
            calcium influx in cortical layer 2/3 pyramidal cells.
  JOURNAL   eLife. 2022 Mar 23;11. pii: 76993. doi: 10.7554/eLife.76993.
  PUBMED    35319464
REFERENCE   2  (bases 1 to 12900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2022-03-23"; volume: "11. pii: 76993. doi"; pages: " 
            10.7554/eLife.76993"
COMMENT     Sequence Label: PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2...
FEATURES             Location/Qualifiers
     source          1..12900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2673..2976
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2978..3252
                     /label=chicken beta-actin promoter
     intron          3253..4269
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4193..4215
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4277..4296
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4358..4367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5183..5185,5186..5896)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGGGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5225..5246)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5486..5505)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5833..5854
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6122..6178
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6242..6259
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6263..6295
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(6299..6307,6308..6322)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             6350..6406
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     7102..7121
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7664..8252
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7717..7737)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8135..8146)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8255..8271)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8256..8272)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8777..8957
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8983..9000)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8989..9213
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9259..9687
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9346..9365)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9556..9577
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9696..9716)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9740..9950
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9762..9958
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9809..9944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9871..9890
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10006..10053
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10072..10446
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10576..10697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10613..10632)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10667..10686
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10746..10762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10746..10762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10759..10781)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10770..10786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10794..10800,10801..10818,10819..10824))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10839..10860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10977..10994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11148..11736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11228..11247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11907..12698,12699..12767))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12530..12549
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12768..12872)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta actgcaggaa tctagttatt aatagtaatc aattacgggg
     2641 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     2701 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     2761 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     2821 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     2881 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     2941 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     3001 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     3061 ttattttgtg cagcgatggg ggcggggggg ggggggcgcg cgccaggcgg ggcggggsgg
     3121 ggsgrggggs ggggsggggs grggcggaga ggtgcggcgg cagccaatca gagcggcgcg
     3181 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     3241 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
     3301 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
     3361 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
     3421 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
     3481 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
     3541 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
     3601 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
     3661 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
     3721 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     3781 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     3841 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggtccc
     3901 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     3961 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     4021 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     4081 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     4141 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     4201 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     4261 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     4321 ttgatttgat accgcgggcc cgggatctcg aggatccgcc accatggttt ctatcgcact
     4381 gcaggctggt tatgacctcc tcggagatgg tagacctgaa agtctgtggc tcggaattgg
     4441 tacactgctg atgctgatcg gaaccttcta cttcctggtt agagcgggcg gagagactga
     4501 caaggatgca cgggagtact acgcagtgac aatcctggtc agcggtattg ccagtgcagc
     4561 ttatctgtca atgttctttg gtattggcct cacagaggtg ccagtaggtg gagagatcct
     4621 gaacatctac tacgcaagat acgcacagtg gctgctgact acaccactgt tgctgctgca
     4681 cctggcactc ctggccaaag ttgatagagt gaccatcggc actctcgttg gcggcgatgc
     4741 tctgatgatt gtcacaggcc tgatagggac actgtcacat actgccattg ccagatactc
     4801 ttggtgcctg ttctcaacta tctgtatgat cgtcgttctg tatgtcctgg ccacttctct
     4861 gcggtcagcc gctaaggaga gaggcccaga ggttgcctca accttcaaca tcctcaccgc
     4921 actggttctg gtcctgtgga ctgcttaccc tatcatctgg attatcggca ccgagggtgc
     4981 cggagttgtg ggcctgggaa tcgaaacttt gctgttcatg gtgctggacg tcaccgccaa
     5041 ggtgggcttc ggttttatcc tgctcaggtc aagaagcatt ctgcaagata cagaagcacc
     5101 tgaaccttca gctggagctg ataagagtag aatcacaagc gaaggcgagt acatccccct
     5161 ggatcaaata gacataaatg tagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     5221 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     5281 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5341 gcccgtgccc tggcccaccc tcgtgaccac cttcggcggc ggcctgatgt gcttcgcccg
     5401 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5461 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     5521 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     5581 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     5641 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     5701 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5761 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     5821 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5881 ggacgagctg tacaaatcaa ggatcacctc tgagggcgag tatatccctc tggatcagat
     5941 cgacattaac gtcggtggaa aatcccgcat aacctctgag ggcgagtaca tccctctgga
     6001 tcaaatcgat ataaacgtgg ggggaaaaag tcgaatcact tctgagggcg agtatatacc
     6061 cctcgatcag attgacatca atgtgggggg cttttgttat gagaatgaag tgggctccgg
     6121 agccacgaac ttctctctgt taaagcaagc aggagacgtg gaagaaaacc ccggtcccct
     6181 gcagaacgag cttgctctta agttggctgg acttgatatt aacaagactg gaggaggttc
     6241 tcatcatcat catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga
     6301 tctgtacgac gatgacgata aggatctcgc aacaatggtc gactcatcac gtcgtaagtg
     6361 gaataaggca ggtcacgcag tcagagctat aggtcggctg agctcacccg tggtttccga
     6421 gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaaggggc tgaggctgaa
     6481 ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca
     6541 gctgcccggc gcctacatcg tggacatcaa gttggacatc gtgtcccaca acgaggacta
     6601 caccatcgtg gaacagtgcg aacgcgccga gggccgccac tccaccggcg gcatggacga
     6661 gctgtacaag ggaggtacag gcgggagtct ggtgagcaag ggcgaggagg ataacatggc
     6721 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     6781 gttcgagatc gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct
     6841 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     6901 gtacggctcc aaggcctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc
     6961 cttccccgag ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca
     7021 cgtgaaccag gactcctccc tgcaggacgg cgtattcatc tacaaggtga agctgcgcgg
     7081 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggctac
     7141 gcgtgacgac ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga
     7201 caaggacggg gatgggacga taacaaccaa ggagcttggg acggtgttcc gatcgctggg
     7261 gcagaacccc acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga
     7321 cggcacattc gacttccctg agttcctgac gatgatggca agaaaaatga atgacacaga
     7381 cagtgaagag gaaattcgcg aagcgttccg cgtgtttgat aaggacggca atggctacat
     7441 cggcgcagca gagcttcgcc acgtgatgac agaccttgga gagaagttaa cagatgagga
     7501 ggttgatgaa atgatcaggg tagcagacat cgatggggat ggtcaggtaa actacgaaga
     7561 gtttgtacaa atgatgacag cgaagaccgg tggtaaaaag aagaaaaaga agtcaaagac
     7621 aaagtgtgta attatgtgag aattcgatat caagcttatc gataatcaac ctctggatta
     7681 cgaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7741 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7801 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7861 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7921 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7981 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     8041 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     8101 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     8161 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     8221 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga
     8281 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     8341 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     8401 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8461 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     8521 aggctacttc cctgattggc agaactacac accagggcca gggatcagat atccactgac
     8581 ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga
     8641 aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     8701 agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct
     8761 gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8821 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8881 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8941 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     9001 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     9061 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     9121 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     9181 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     9241 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     9301 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     9361 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     9421 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     9481 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     9541 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     9601 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     9661 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     9721 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9781 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9841 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     9901 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9961 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
    10021 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
    10081 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
    10141 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
    10201 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
    10261 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
    10321 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
    10381 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
    10441 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
    10501 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
    10561 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10621 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10681 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
    10741 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    10801 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    10861 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    10921 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    10981 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    11041 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    11101 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    11161 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    11221 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    11281 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    11341 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    11401 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    11461 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    11521 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11581 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    11641 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    11701 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    11761 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    11821 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    11881 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    11941 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    12001 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    12061 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    12121 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    12181 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    12241 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    12301 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    12361 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    12421 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    12481 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    12541 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12601 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    12661 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    12721 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    12781 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    12841 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
