LOCUS       sequence-357485-        7593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for conversion of Gal4 line into ShineGal4 drivers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   di Pietro F, Herszterg S, Huang A, Bosveld F, Alexandre C, Sancere 
            L, Pelletier S, Joudat A, Kapoor V, Vincent JP, Bellaiche Y
  TITLE     Rapid and robust optogenetic control of gene expression in 
            Drosophila.
  JOURNAL   Dev Cell. 2021 Dec 20;56(24):3393-3404.e7. doi: 
            10.1016/j.devcel.2021.11.016. Epub 2021 Dec 7.
  PUBMED    34879263
REFERENCE   2  (bases 1 to 7593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.11.016"; journalName: "Dev Cell"; date: 
            "2021-12-20- 20"; volume: "56"; issue: "24"; pages: "3393-3404.e7"
COMMENT     Sequence Label: pTV-Gal4DBD2xHighMag1
FEATURES             Location/Qualifiers
     source          1..7593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             686..1126
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    2819..2852
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3056..3766
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3201..3219)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    3886..4007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3923..3942)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3977..3996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4016..4049
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(5405..5423)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5444..5460)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5444..5460)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5457..5479)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5468..5484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5492..5498,5499..5516,5517..5522))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5537..5558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5675..5692)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5846..6434)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5926..5945)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6605..7396,7397..7465))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7228..7247
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7466..7570)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctgaattc
      661 gttgactgta tcgattgact cggagatgaa gctactgtct tctatcgaac aagcatgcga
      721 tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct
      781 gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc tgactagggc
      841 acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc tactgatttt
      901 tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata taaaagcatt
      961 gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag atagattggc
     1021 ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg cgacatcatc
     1081 atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgggag gcggaggctc
     1141 cggtggtggt ggaagtggcg gtggcggatc ccataccctg tacgcgcccg gaggttatga
     1201 cattatgggt tatctcgatc agatcggaaa ccgtcccaat ccgcaggttg agctgggacc
     1261 agtggatacg tcctgtgctt tgattctctg tgatctgaaa cagaaagata cccctattgt
     1321 ttatgcaagt gaggcctttc tgtatatgac cggttactcc aacgcggagg tgttgggccg
     1381 gaattgtcgt tttctgcagt cgccagacgg aatggtcaaa cctaaatcca cacgcaagta
     1441 tgtggacagc aatactataa atacgattcg aaaggcaata gaccgcaacg ctgaagtcca
     1501 ggtggaggtc gttaatttca agaagaatgg acaaaggttc gtcaacttct tgacgatcat
     1561 cccggtccgt gacgagacag gtgagtatcg atatagtatg ggctttcaat gcgaaacgga
     1621 aggtggaggt ggcagtggag gaggaggaag cggtggcggt ggcagtcata ccttgtatgc
     1681 accaggcggt tacgacataa tgggctattt ggaccaaatc ggaaacaggc cgaaccccca
     1741 agtcgagctc ggaccagtag atacaagctg cgcgctcatc ttgtgtgatt tgaaacaaaa
     1801 ggatacccct atagtgtatg ccagcgaagc attcctctac atgacaggct acagcaacgc
     1861 agaagttctg ggaaggaact gtcgatttct ccagtcgcca gacggcatgg tcaaaccgaa
     1921 atcgactcgg aagtatgtag atagtaatac aatcaacacg atacgtaaag ccattgacag
     1981 gaatgcggaa gtacaagttg aggttgttaa ttttaagaaa aatggccaga ggtttgttaa
     2041 ctttctcaca ataatacctg tgagggacga aaccggcgag tacaggtaca gcatgggatt
     2101 ccaatgtgag acggaataat ctagaaggcc tggcgcgccg ctagcatgaa tcgtttttaa
     2161 aataacaaat caattgtttt ataatattcg tacgattctt tgattatgta ataaaatgtg
     2221 atcattagga agattacgaa aaatataaaa aatatgagtt ctgtgtgtat aacaaatgct
     2281 gtaaacgcca caattgtgtt tgttgcaaat aaacccatga ttatttgatt aaaattgttg
     2341 ttttctttgt tcatagacaa tagtgtgttt tgcctaaacg tgtactgcat aaactccatg
     2401 cgagtgtata gcgagctagt ggctaacgct tgccccacca aagtagattc gtcaaaatcc
     2461 tcaatttcat caccctcctc caagtttaac atttggccgt cggaattaac ttctaaagat
     2521 gccacataat ctaataaatg aaatagagat tcaaacgtgg cgtcatcgtc cgtttcgacc
     2581 atttccgaaa agaactcggg cataaactct atgatttctc tggacgtggt gttgtcgaaa
     2641 ctctcaaagt acgcagtcag gaacgtgcgc gacatgtcgt cgggaaactc gcgcggaaac
     2701 atgttgttgt aaccgaacgg gtcccatagc gccaaaacca aatctgccag cgtcaataga
     2761 atggcacgat gccgacaatg gagctggctt ggatagcgat tcgagttaac ggtaccccat
     2821 aacttcgtat aatgtatgct atacgaagtt atcgtacggc tatacgaagt tatgcccgga
     2881 tctaattcaa ttagagacta attcaattag agctaattca attagcgccg gagtataaat
     2941 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     3001 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc ggcccatggt
     3061 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3121 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3181 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3241 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3301 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3361 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     3421 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     3481 gaagaaaacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     3541 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     3601 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     3661 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     3721 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagcgc cgcgactctg
     3781 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     3841 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     3901 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3961 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttagcc ccggtataac
     4021 ttcgtataat gtatgctata cgaagttatc actagtattg actcggcagc tcatcatgat
     4081 aactccacaa ttccgttgga ttttatgccc agggatgctc ttcatggatt tgattggtct
     4141 gaagaggatg acatgtcgga tggcttgccc ttcctgaaaa cggaccccaa caataatggg
     4201 ttctttggcg acggttctct cttatgtatt cttcgatcta ttggctttaa accggaaaat
     4261 tacacgaact ctaacgttaa caggctcccg accatgatta cggatagata cacgttggct
     4321 tctagatcca caacatcccg tttacttcaa agttatctca ataattttca cccctactgc
     4381 cctatcgtgc actcaccgac gctaatgatg ttgtataata accagattga aatcgcgtcg
     4441 aaggatcaat ggcaaatcct ttttaactgc atattagcca ttggagcctg gtgtatagag
     4501 ggggaatcta ctgatataga tgttttttac tatcaaaatg ctaaatctca tttgacgagc
     4561 aaggtcttcg agtcaggttc tataattttg gtgacagccc tacatcttct gtcgcgatat
     4621 acacagtgga ggcagaaaac aaatactagc tataattttc acagcttttc cataagaatg
     4681 gccatatcat tgggcttgaa tagggacctc ccctcgtcct tcagtgatag cagcattctg
     4741 gaacaaagac gccgaatttg gtggtctgtc tactcttggg agatccaatt gtccctgctt
     4801 tatggtcgat ccatccagct ttctcagaat acaatctcct tcccttcttc tgtcgacgat
     4861 gtgcagcgta ccacaacagg tcccaccata tatcatggca tcattgaaac agcaaggctc
     4921 ttacaagttt tcacaaaaat ctatgaacta gacaaaacag taactgcaga aaaaagtcct
     4981 atatgtgcaa aaaaatgctt gatgatttgt aatgagattg aggaggtttc gagacaggca
     5041 ccaaagtttt tacaaatgga tatttccacc accgctctaa ccaatttgtt gaaggaacac
     5101 ccttggctat cctttacaag attcgaactg aagtggaaac agttgtctct tatcatttat
     5161 gtattaagag attttttcac taattttacc cagaaaaagt cacaactaga acaggatcaa
     5221 aatgatcatc aaagttatga agttaaacga tgctccatca tgttaagcga tgcagcacaa
     5281 agaactgtta tgtctgtaag tagctatatg gacaatcata atgtcacccc atattttgcc
     5341 tggaattgag acgtcgatta attaagcacg cgtgcaccgg tagatctcgt accagctttt
     5401 gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg
     5461 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5521 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5581 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5641 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5701 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5761 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5821 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5881 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5941 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6001 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6061 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6121 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6181 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6241 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6301 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6361 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6421 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6481 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6541 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6601 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6661 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6721 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6781 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6841 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6901 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6961 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7021 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7081 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7141 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7201 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7261 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7321 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7381 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7441 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7501 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7561 gggttccgcg cacatttccc cgaaaagtgc cac
//
