LOCUS       sequence-357491-        9505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9505)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 9505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: pMT-EGFP-CTAP-PrP-SBP_W
FEATURES             Location/Qualifiers
     source          1..9505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(694..711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(865..1453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(945..964)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1624..2415,2416..2484))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2247..2266
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2485..2589)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2657..2675
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4071..4350
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             4396..5421
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPRLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          5784..5849
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5979..5999
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6424..6558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6461..6480)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6515..6534
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             join(6999..7001,7002..7004,7005..7715)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6999..7001,7002..7004,7005..7715)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7044..7065)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7305..7324)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7652..7673
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7722..7832
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             7839..7862
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7896..8069
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             8070..8243
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
ORIGIN
        1 gcggccgccg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
       61 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
      121 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat
      181 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg
      241 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac
      301 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga
      361 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc
      421 atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg
      481 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
      541 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
      601 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
      661 ctggaaacgc ggaagtcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      721 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      781 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      841 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      901 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      961 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1021 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1081 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1141 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1201 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1261 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     1321 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1381 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1441 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1501 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1561 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1621 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1681 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1741 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     1801 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1861 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1921 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     1981 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2041 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2101 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2161 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2221 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     2281 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2341 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2401 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2461 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2521 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2581 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     2641 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattcacatt
     2701 tgtacgaatt ttttttttat caaaagttcg agtttttcac caatttcctc atcaaccgag
     2761 caaggcaaac ggctttgaat aatatggtgt tatatataca tatatcaaat cgctgctgac
     2821 tgcgtgattg atggccccaa gattacatat tatcgaatca ggattcagaa ggagatcaat
     2881 gtcaaatgcg gacaggaaca tgaaagacgc ctgttatgcg caattaaaaa tttgggttta
     2941 attgctgtgg aaactgttgt tggcggcatc ttaagttcct gtttaacaac atcaactact
     3001 tatgtacgta gaagcgttta agccatttgc atacagatga gaactggctt ttgtgctaat
     3061 cagtcaagat gactccgatg atgatgactc attacctgac cagttttcgc tgctttcttt
     3121 tcaacaacta cttgtatatg tattgtatcc aatagcaata cattgaattt ccatggtcta
     3181 gtcacgtatt atcatttaat tgacaccaag tcgtgttatt gttgagctat cgagttcagc
     3241 tcaaacattt cttattccca tgaataagcc ggcaaaaata tgcaatctat gaaagttaat
     3301 ataagcaaac cttactttga ctcaatacca atgcactttg tgtcgatagg ttcacgcaat
     3361 tgaggcgatt attccgataa cccaagcgat tgactgttcc cgtttcgatt ccaattgaaa
     3421 tttggaaatg tacaatagtt ttgctatatg ctgtcaagta cgctcttatc ttctctgggt
     3481 tttcttcaga gtttcgaaac gcttcttctt ttttttgttt tttttttttt ggaatctcgt
     3541 attttggaag gggctcccct ctggaatttg ttacactgtc gttatcattg cgaacaagcg
     3601 gcccgaagct atcagcgact ttaacattta caatgcactt ttttacgacc aattaaatgt
     3661 acattttcct ttcttcgccc gttgataagc gaacgcgatg tggcgcaggc aatgtgttgc
     3721 tcttgcgaca caaacgcaat caaaatggat tcaatttcgc tttttcccag tgaaacgaag
     3781 aacgaaccga ccatcatgat atgctcctct gcatgttgcg tattgaatca atgacaattt
     3841 caattaagcc gcccgttcgt catgcgtttt cgtgcgcttc gaaatgctga taacgctgct
     3901 gtcctccaac tgctttgcat gtggacacaa ttccatttat ttaattcttt tatttggatc
     3961 ggttaaatta aaaagcgcct tgttacgcat ttaacgttgt ttccggtgcg tggtggtttc
     4021 atgcttctgg gaacggcaaa tgggtttagg attgggaacc cctcatcatc tgttggaata
     4081 tactattcaa cctacaaaag taacgttaaa caacactact ttatatttga tatgaatggc
     4141 cacacctttt atgccataaa acatattgta agagaatacc actcttttta ttccttcttt
     4201 ccttcttgta cgttttttgc tgtaagtagg tcgtggtgct ggtgttgcag ttgaaataac
     4261 ttaaaatata aatcataaaa ctcaaacata aacttgacta tttatttatt tattaagaaa
     4321 ggaaatataa attataaatt acaacaggtt atggacctgc agccaagctt ggcgctcgtc
     4381 cgggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     4441 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     4501 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     4561 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     4621 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     4681 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     4741 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     4801 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     4861 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     4921 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     4981 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     5041 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     5101 aggccgcggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     5161 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     5221 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     5281 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     5341 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     5401 cgtccgaggg caaaggaata gagtagatgc cgaccgaaca agagctgatt tcgagaacgc
     5461 ctcagccagc aactcgcgcg agcctagcaa ggcaaatgcg agagaacggc cttacgcttg
     5521 gtggcacagt tctcgtccac agttcgctaa gctcgctcgg ctgggtcgcg ggagggccgg
     5581 tcgcagtgat tcaggccctt ctggattgtg ttggtcccca gggcacgatt gtcatgccca
     5641 cgcactcggg tgatctgact gatcccgcag attggagatc gccgcccgtg cctgccgatt
     5701 gggtgcagat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     5761 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     5821 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     5881 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg
     5941 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag
     6001 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta
     6061 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca
     6121 agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata
     6181 acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc
     6241 aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt
     6301 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     6361 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     6421 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6541 tcttatcatg tctggatcaa ttcgttgcag gacaggatgt ggtgcccgat gtgactagct
     6601 ctttgctgca ggccgtccta tcctctggtt ccgataagag acccagaact ccggcccccc
     6661 accgcccacc gccaccccca tacatatgtg gtacgcaagt aagagtgcct gcgcatgccc
     6721 catgtgcccc accaagagct ttgcatccca tacaagtccc caaagtggag aaccgaacca
     6781 attcttcgcg ggcagaacaa aagcttctgc acacgtctcc actcgaattt ggagccggcc
     6841 ggcgtgtgca aaagaggtga atcgaacgaa agacccgtgt gtaaagccgc gtttccaaaa
     6901 tgtataaaac cgagagcatc tggccaatgt gcatcagttg tggtcagcag caaaatcaag
     6961 tgaatcatct cagtgcaact aactcgagcc cgggtaccat ggtgagcaag ggcgaggagc
     7021 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     7081 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     7141 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     7201 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     7261 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     7321 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     7381 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     7441 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     7501 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     7561 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     7621 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     7681 ccgggatcac tctcggcatg gacgagctgt acaaggatat cgacgagaag accaccggct
     7741 ggcggggcgg ccacgtggtg gagggcctgg ccggcgagct ggagcagctg cgggccaggc
     7801 tggagcacca ccctcagggc cagcgggagc ccgaactact ggaagttctg ttccaggggc
     7861 ccaaaaccgc ggctcttgcg caacacgatg aagccgtgga caacaaattc aacaaagaac
     7921 aacaaaacgc gttctatgag atcttacatt tacctaactt aaacgaagaa caacgaaacg
     7981 ccttcatcca aagtttaaaa gatgacccaa gccaaagcgc taacctttta gcagaagcta
     8041 aaaagctaaa tgatgctcag gcgccgaaag tagacaacaa attcaacaaa gaacaacaaa
     8101 acgcgttcta tgagatctta catttaccta acttaaacga agaacaacga aacgccttca
     8161 tccaaagttt aaaagatgac ccaagccaaa gcgctaacct tttagcagaa gctaaaaagc
     8221 taaatgatgc tcaggcgccg aaagtagacg cgaattccgc ggggaagtca acctgaggat
     8281 ccactagttc tagtcgacca tgaagatcaa gatcattgcc ccgccagagc gcaagtactc
     8341 tgtctggatc ggtggctcca tcctggcttc gctgtccacc ttccagcaga tgtggatctc
     8401 caagcaggag tacgacgagt ccggcccctc cattgtgcac cgcaagtgct tctaagaagg
     8461 atcgcttgtc tgggcaagag gatcaggatc gggatggtct tgattctgct ggaggaggag
     8521 gaggagaagt cgaggaagca gcagcgaaag tgcaagtgcg agtggtggaa gtttggagtg
     8581 cagcacaaca aaatcaacaa caacaccaac tacaagatga aaagagcgga accacctgca
     8641 caccatcatc actatcatca tcgttttggg cgcatgttgt gtggttccag cgtattaata
     8701 taattaattt attccacatg agatatgata tgatatacta tgtatttttt gttttttttt
     8761 tatttgtaaa cctttaatat aacaagaact acaaaaaatg aaaatgagcg aaaatgcata
     8821 ttctgccatt ccacacacac accaacaaca cccaacacac gcacacccac aagcttacac
     8881 acacacattc gcggcatgac aaggacatca agataaagaa gaacttaaag aagatatttc
     8941 ccaaagcgca aaaagaacac acacacattg caaaacacaa acaacacact agcgttttgt
     9001 acaattcgtc agcaacctta tgtattattt tttaattatg atgtaattat aaacaaagtg
     9061 aaacaaaaat atgaaaacaa aaaggaaaat caaatctgtc ttctctttct cccgctctcc
     9121 tcgctctctg ctgctaacct cgccctctcc tctctcatct ttttgtctgt ctctcttcca
     9181 catttttgcc ggccggcaaa ataataaccc acacacactc acacttggct gcagtttcgc
     9241 gtgcgatatt cacacacatt caagcataca agcatacata catatgtatt ttttttttat
     9301 ttgtacactt ttctaattgc atgcgtatcg attgataagt ttacgcctga aaatgttaat
     9361 taaaatgtga aaatgcaact gaaaaactga tgaaatgaaa caacaacaag cgaacaattt
     9421 gctacatgtg tattgtctaa caaccgttac tgcaacggtt gcttcggaaa agggtgaaga
     9481 ggaagagggc acgaggtcga ctaga
//
