LOCUS       sequence-357493-       10053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmids for production of LV-based TFactivity reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Abe H, Abe K
  TITLE     PCR-based profiling of transcription factor activity in vivo by a 
            virus-based reporter battery.
  JOURNAL   iScience. 2022 Feb 14;25(3):103927. doi: 10.1016/j.isci.2022.103927.
            eCollection 2022 Mar 18.
  PUBMED    35281741
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-14"; volume: "25(3):103927. doi"; pages: " 
            10.1016/j.isci.2022.103927. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pLVTFrep2-creb
FEATURES             Location/Qualifiers
     source          1..10053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(164..183)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        355..734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        736..934
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     889..909
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             952..1132
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1179..1304
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1797..2030
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2215..2259
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2533..2650
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2731..2850)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(2860..2880)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2896..3603)
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
     regulatory      3600..3609
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3636..3667)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3862..3871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3866..3875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4251..4270
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4351..4728
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4756..4765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4765..4788
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4798..4821
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4868..5456
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4921..4941)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5339..5350)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5975..6155
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6181..6198)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6187..6411
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6457..6885
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6544..6563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6754..6775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6894..6914)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6899..7228
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7079..7214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7141..7160
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7276..7323
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7342..7716
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7846..7967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7883..7902)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7937..7956
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8016..8032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8016..8032)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8029..8051)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8040..8056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8064..8070,8071..8088,8089..8094))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8109..8130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8247..8264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8418..9006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8498..8517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9177..9968,9969..10037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9800..9819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10038..10053,1..89))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
       61 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
      121 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
      181 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      241 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      301 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      361 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      601 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      661 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      721 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      781 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      841 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      901 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      961 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     1021 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     1081 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1141 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1201 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1261 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1321 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1381 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1441 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1501 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1561 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1621 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1681 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1741 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1801 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1861 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1921 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1981 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     2041 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     2101 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2161 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2221 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2281 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2341 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2401 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2461 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctac aaatggcagt
     2521 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2581 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2641 tcaaaatttt cgggtttatt acagggacag cagaaattca ctttgaattc acaaaagctg
     2701 ggtaccgggc cccccctcga gtctagatta gacgttgatc ctggcgctgg cgcaagcagc
     2761 agggtgtcta tccatgccgc tctcctgggc gcagctcatg ggcagggttc ctgcggcctg
     2821 ttcctccacc tctggaggga agccgtgaga atttcctact accttacgct tcttctttgg
     2881 agctactgta caacccttat aaagctcgtc catgccgtcg gtggagttgc gggccacggc
     2941 gatctcgtaa acggtcacct tgttgtagtc cttgtcgtgg ttcaggatct cgatgcggtg
     3001 gtccacaaag tgatagtcgg gcagcttcac cgccttcttg gccctgtaga tggtcttgaa
     3061 gtcaacgcgg tggtggccgc cgccctccag cagcagcttc atcttgacgt cgcccttcag
     3121 cacgccgtcg cgcacgtaca tcctctcggt ggaggcgtcc cagccggtgg tctccttctg
     3181 catcacgggg ccgttggggg ggaagttctc gcccttgagg tgtatctcgt agatgaagga
     3241 gtcctcctcc atggagatgt cggacttcac cttcacgatg cccttgtcct cgaaggtcat
     3301 ggtgcgctcc caagagtagc cctcggggaa ggactgcttg aagtagttgg ggatgtcgtc
     3361 ggggtacttg gtgaaggccc tgttgccgta actgaacgcg gtggtcagaa tgtcgtagga
     3421 gaagggcagg ggggctccct ccttcacctc caggttgatg gtgttggtgc cgtcgtaggg
     3481 cttgccctcg ccctcgccct cgatcacgaa ggcgtggcca ttcacgttgc cctccatctt
     3541 cagcttgatc ttcatgtcgg gcttgattac gcccattgtg gtctcctcgc ccttgctcac
     3601 catggtggcg accggtacag taccggattg ccaagctgga agtcgagctt ccattatata
     3661 ccctctagtg tctaagctta tctgttctcc cattgacgtc aatggggtgt ctgaaagacg
     3721 tcacagtatg acggccatgg gatctggcag ctgacgtcac tgtctggtgc ggatcccccc
     3781 caaagccccc ggggctgtaa ttgcgtccct ctcccgctag ggggcagcag cgagccgccc
     3841 ggggcggcgc gaattcccgc ggccgccatg gcggccggga gcatgcgacg tcgggcccaa
     3901 tctgcagcac gtgccgcggt ggcggccgcc cggtaggcgc caaccggctc cgttctttgg
     3961 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     4021 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     4081 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     4141 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     4201 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     4261 gcttcaaaag cgcacgtctg tcgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     4321 ctcacggtgt tagcgctacc ggccgccacc atgccagagc cagcgaagtc tgctcccgcc
     4381 ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc
     4441 aagcgcagcc gcaaggagag ctattccatc tatgtgtata aggttctgaa gcaggtccac
     4501 cctgacaccg gcatttcgtc caaggcgatg ggcatcatga attcgtttgt gaacgacatt
     4561 ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg gtcgaccatc
     4621 acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggcgaagcac
     4681 gccgtgtccg agggtactaa ggccatcacc aagtacacca gtgctaagga cccaccagtc
     4741 gccaccttac cggtcgccac catggactac aaggatgacg acgacaaagg tggagcagac
     4801 tataaggacg atgatgacaa ggctggtcat atggctagaa attcctcgag ggatcaagct
     4861 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4921 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4981 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5041 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5101 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5161 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5221 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     5281 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5341 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5401 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     5461 ataccgtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     5521 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     5581 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5641 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     5701 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     5761 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     5821 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     5881 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     5941 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     6001 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6061 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6121 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     6181 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6241 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6301 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6361 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6421 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6481 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6541 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6601 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6661 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6721 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6781 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6841 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6901 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6961 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7021 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7081 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7141 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7201 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7261 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     7321 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     7381 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     7441 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     7501 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     7561 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     7621 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     7681 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     7741 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     7801 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     7861 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7921 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     7981 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     8041 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8101 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8161 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8221 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8281 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8341 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8401 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8461 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8521 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8581 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8641 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8701 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8761 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8821 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8881 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8941 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9001 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9061 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9481 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9781 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10021 aaatgttgaa tactcatact cttccttttt caa
//
