LOCUS       sequence-357494-        9997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmids for production of LV-based TFactivity reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Abe H, Abe K
  TITLE     PCR-based profiling of transcription factor activity in vivo by a 
            virus-based reporter battery.
  JOURNAL   iScience. 2022 Feb 14;25(3):103927. doi: 10.1016/j.isci.2022.103927.
            eCollection 2022 Mar 18.
  PUBMED    35281741
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-14"; volume: "25(3):103927. doi"; pages: " 
            10.1016/j.isci.2022.103927. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pLVTFrep3-creb
FEATURES             Location/Qualifiers
     source          1..9997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     218..237
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             726..767
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    794..1382
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(847..867)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1265..1276)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1901..2081
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2107..2124)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2113..2337
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2383..2811
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2470..2489)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2680..2701
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2820..2840)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2825..3154
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3005..3140
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3067..3086
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3202..3249
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3268..3642
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3772..3893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3809..3828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3863..3882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3942..3958)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3942..3958)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3955..3977)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3966..3982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3990..3996,3997..4014,4015..4020))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4035..4056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4173..4190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4344..4932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4424..4443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5103..5894,5895..5963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5726..5745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5964..6068)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6143..6162)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6334..6713
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6715..6913
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6868..6888
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6931..7111
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7158..7283
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7776..8009
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8194..8238
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8512..8629
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8710..8829)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(8839..8859)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8872..9567)
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     regulatory      9564..9573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9600..9631)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      9826..9835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9830..9839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
       61 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
      121 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
      181 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
      241 acgtctgtcg cgctgttctc ctcttcctca tctccgggcc tttcgacctc acggtgttag
      301 cgctaccggc cgccaccatg cccgaacctg ccaaatccgc cccagcccca aagaagggaa
      361 gcaagaaagc agtgaccaaa gcacagaaaa agggcggaaa aaagcggaaa cggagtcgga
      421 aggaaagtta ctctatttac gtctataaag tcctgaaaca ggtgcatccc gatacaggaa
      481 tctcctccaa agcgatggga attatgaact ccttcgtcaa tgacatcttt gaacggattg
      541 ctggcgaagc ctctcggctg gcccactata ataaacggtc cactattact tccagagaaa
      601 ttcaaaccgc cgtgcggctg ctcctgccag gcgaactcgc gaaacatgcc gtgtctgaag
      661 gcaccaaagc tattacaaaa tatacaagtg ccaaagaccc cccagtgggg caagcccacg
      721 gagctggcaa gcctatccca aatcccctgc tgggactgga ctctacataa ctcgagggat
      781 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
      841 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
      901 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
      961 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     1021 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     1081 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     1141 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     1201 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     1261 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     1321 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     1381 gcatcgatac cgtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     1441 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     1501 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     1561 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     1621 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     1681 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     1741 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     1801 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     1861 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     1921 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     1981 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2041 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     2101 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     2161 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     2221 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     2281 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     2341 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     2401 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     2461 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     2521 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     2581 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     2641 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     2701 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     2761 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     2821 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2881 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     2941 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3001 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3061 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     3121 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     3181 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     3241 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     3301 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     3361 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     3421 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     3481 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     3541 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     3601 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     3661 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     3721 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     3781 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3841 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3901 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     3961 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4021 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4081 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4141 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4201 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4261 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4321 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4381 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4441 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4501 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4561 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4621 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4681 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4741 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4801 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4861 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4921 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4981 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5041 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5101 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5161 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5221 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5281 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5341 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5401 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5461 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5521 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5581 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5641 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5701 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5761 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5821 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5881 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5941 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6001 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6061 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     6121 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     6181 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     6241 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     6301 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     6361 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     6421 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     6481 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     6541 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     6601 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     6661 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     6721 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     6781 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     6841 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     6901 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     6961 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7021 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7081 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7141 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7201 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     7261 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     7321 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     7381 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     7441 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     7501 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     7561 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     7621 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     7681 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     7741 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     7801 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     7861 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     7921 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     7981 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     8041 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     8101 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     8161 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     8221 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     8281 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     8341 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     8401 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     8461 agatccattc gattagtgaa cggatctaca aatggcagta ttcatccaca attttaaaag
     8521 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     8581 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     8641 cagggacagc agaaattcac tttgaattca caaaagctgg gtaccgggcc ccccctcgag
     8701 tctagattag acgttgatcc tggcgctggc gcaagcagca gggtgtctat ccatgccgct
     8761 ctcctgggcg cagctcatgg gcagggttcc tgcggcctgt tcctccacct ctggagggaa
     8821 gccgtgagaa tttcctacta ccttacgctt cttctttgga gccgtgtaca cttcttcacc
     8881 ggcatctgca tccggggtct tgaaggcgtg ctggtactcc acgatgccca gctcggtgtt
     8941 gctgtgatcc tcctccacgc ggcggaaggc gaacatgggg cccccgttct gcaggatgct
     9001 ggggtggatg gcgctcttga agtgcatgtg gctgtccacc acggagctgt agtagccgcc
     9061 gtcgcgcagg ctgaaggtgc gggtgaagct gccatccaga tcgttatcgc ccatggggtg
     9121 caggtgctcc acggtggcgt tgctgcggat gatcttgtcg gtgaagatca cgctgtcctc
     9181 ggggaagccg gtgcccatca ccttgaagtc gccgatcacg cggccggcct cgtagcggta
     9241 gctgaagctc acgtgcagca cgccgccgtc ctcgtacttc tcgatgcggg tgttggtgta
     9301 gccgccgttg ttgatggcgt gcaggaaggg gttctcgtag ccgctggggt aggtgccgaa
     9361 gtggtagaag ccgtagccca tcacgtggct cagcaggtag gggctgaagg tcagggcgcc
     9421 tttggtgctc ttcatcttgt tggtcatgcg gccctgctcg ggggtgccct ctccgccgcc
     9481 caccagctcg aactccacgc cgttcagggt gccggtgatg cggcactcga tctccatggc
     9541 gggcaggccg ctctcgtcgc tctccatggt ggcgaccggt acagtaccgg attgccaagc
     9601 tggaagtcga gcttccatta tataccctct agtgtctaag cttatctgtt ctcccattga
     9661 cgtcaatggg gtgtctgaaa gacgtcacag tatgacggcc atgggatctg gcagctgacg
     9721 tcactgtctg gtgcggatcc cccccaaagc ccccggggct gtaattgcgt ccctctcccg
     9781 ctagggggca gcagcgagcc gcccggggcg gcgcgaattc ccgcggccgc catggcggcc
     9841 gggagcatgc gacgtcgggc ccaatctgca gcacgtgccg cggtggcggc cgcccggtag
     9901 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     9961 caggaagttc ccccccgccc cgcagctcgc gtcgtgc
//
