LOCUS       sequence-357496-       10030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmids for production of LV-based TFactivity reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Abe H, Abe K
  TITLE     PCR-based profiling of transcription factor activity in vivo by a 
            virus-based reporter battery.
  JOURNAL   iScience. 2022 Feb 14;25(3):103927. doi: 10.1016/j.isci.2022.103927.
            eCollection 2022 Mar 18.
  PUBMED    35281741
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-14"; volume: "25(3):103927. doi"; pages: " 
            10.1016/j.isci.2022.103927. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pLVTFrep4-creb
FEATURES             Location/Qualifiers
     source          1..10030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(138..157)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        329..708
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        710..908
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..883
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             926..1106
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1153..1278
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1771..2004
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2189..2233
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2507..2624
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2705..2824)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(2834..2854)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3541..3550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3577..3608)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3803..3812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3807..3816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4192..4211
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4700..4729
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4745..4774
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    4819..5407
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4872..4892)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5290..5301)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5926..6106
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6132..6149)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6138..6362
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6408..6836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6495..6514)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6705..6726
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6845..6865)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6850..7179
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7030..7165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7092..7111
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7227..7274
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7293..7667
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7797..7918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7834..7853)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7888..7907
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7967..7983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7967..7983)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7980..8002)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7991..8007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8015..8021,8022..8039,8040..8045))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8060..8081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8198..8215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8369..8957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8449..8468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9128..9919,9920..9988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9751..9770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9989..10030,1..63))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
       61 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
      121 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
      181 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
      241 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
      301 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
      361 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      421 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      481 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      541 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      601 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      661 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      721 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      781 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      841 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      901 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
      961 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     1021 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     1081 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     1141 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     1201 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     1261 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     1321 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1381 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1441 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1501 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1561 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1621 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     1681 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     1741 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     1801 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1861 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1921 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     1981 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     2041 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     2101 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     2161 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     2221 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     2281 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     2341 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     2401 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     2461 cattcgatta gtgaacggat ctacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2521 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2581 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2641 acagcagaaa ttcactttga attcacaaaa gctgggtacc gggccccccc tcgagtctag
     2701 attagacgtt gatcctggcg ctggcgcaag cagcagggtg tctatccatg ccgctctcct
     2761 gggcgcagct catgggcagg gttcctgcgg cctgttcctc cacctctgga gggaagccgt
     2821 gagaatttcc tactacctta cgcttcttct ttggagccgt gtacaccaga aacagatggt
     2881 ggcgtccctc ggttctctcg tactgctcca caatggtata atcctcgtta tgggaggtga
     2941 tatccagctt tgtgtccaca tagtagtatc cggggagctg cacgggtttc tttgccatgt
     3001 agatagattt gaactccacc aggtaatggc cgccatcctt cagtttcagg gctttgtgaa
     3061 tttcgccctt cagcacgcca tctctggggt acagtctctc ggtggaggct tcccatccca
     3121 tggtcttttt ctgcatcact ggtccatcgg agggaaaatt cactccaata aatttcactt
     3181 tgtaaatgaa gcatccgtcc tggagggagg aatcctgtgt cacggtcgcc actcctccat
     3241 cttcaaaatt catcactctt tcccacttaa agccctcagg gaaggacagc tttttatagt
     3301 cgggaatatc ggcgggatgc ttcacataca ccttggagcc gtactggaac tgaggggaca
     3361 gaatatccca ggcgaagggc agagggccgc ctttggtcac tttcagcttc acggtgttat
     3421 ggccctcata ggggcgtccc tctccttcgc cctcaatttc gaactcatgt ccattcactg
     3481 tgccttccat gcgcaccttg aatctcataa attccttgat cacgttcttg gaggacctca
     3541 ccatggtggc gaccggtaca gtaccggatt gccaagctgg aagtcgagct tccattatat
     3601 accctctagt gtctaagctt atctgttctc ccattgacgt caatggggtg tctgaaagac
     3661 gtcacagtat gacggccatg ggatctggca gctgacgtca ctgtctggtg cggatccccc
     3721 ccaaagcccc cggggctgta attgcgtccc tctcccgcta gggggcagca gcgagccgcc
     3781 cggggcggcg cgaattcccg cggccgccat ggcggccggg agcatgcgac gtcgggccca
     3841 atctgcagca cgtgccgcgg tggcggccgc ccggtaggcg ccaaccggct ccgttctttg
     3901 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     3961 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     4021 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     4081 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     4141 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     4201 cgcttcaaaa gcgcacgtct gtcgcgctgt tctcctcttc ctcatctccg ggcctttcga
     4261 cctcacggtg ttagcgctac cggtcgccac catgagtggc cgcggaaaac agggcggaaa
     4321 ggctcgggca aaggcaaaaa ctcggtccag ccgggctggc ctccagtttc cagtcggcag
     4381 ggtccaccgg ctgctgcgga aaggaaatta cgctgagcgg gtcggggctg gcgcgccggt
     4441 gtacctggcc gccgtcctgg aatatctgac cgctgaaatt ctggaactgg cgggcaacgc
     4501 cgcccgcgac aacaagaaga cccgcatcat tccacggcat ttccagctcg ccatcaggaa
     4561 cgacgaagaa ctgaataagc tgctgggcaa agtaacaatt gcccaaggcg gagtgctgcc
     4621 aaatattcaa gccgtcttgc tgcctaaaaa gactgaatcc caccacaagg ccaaaggcaa
     4681 gggctatctg ggcggaggcg agcagaaact gatttctgaa gaagacctcg ctgtaggttc
     4741 aggtgaacaa aagctcatca gcgaggagga cctggctagc tagctctaga aattcctcga
     4801 gggatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4861 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4921 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4981 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5041 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5101 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5161 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     5221 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5281 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5341 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5401 tccccgcatc gataccgtcg agacctagaa aaacatggag caatcacaag tagcaataca
     5461 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     5521 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5581 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5641 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     5701 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     5761 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     5821 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     5881 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     5941 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6001 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6061 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     6121 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6181 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6241 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6301 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     6361 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     6421 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6481 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6541 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6601 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6661 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6721 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6781 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     6841 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     6901 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     6961 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7021 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7081 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     7141 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     7201 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     7261 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     7321 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     7381 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     7441 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     7501 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     7561 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     7621 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     7681 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     7741 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     7801 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7861 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7921 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7981 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8041 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8101 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8161 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8221 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8281 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8821 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9061 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9121 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9181 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9241 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9301 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9361 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9421 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9481 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9541 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9601 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9661 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9721 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9781 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9841 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9901 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9961 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10021 atcagggtta
//
