LOCUS       sequence-357500-       10034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmids for production of LV-based TFactivity reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Abe H, Abe K
  TITLE     PCR-based profiling of transcription factor activity in vivo by a 
            virus-based reporter battery.
  JOURNAL   iScience. 2022 Feb 14;25(3):103927. doi: 10.1016/j.isci.2022.103927.
            eCollection 2022 Mar 18.
  PUBMED    35281741
REFERENCE   2  (bases 1 to 10034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-14"; volume: "25(3):103927. doi"; pages: " 
            10.1016/j.isci.2022.103927. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pLVTFrep6-creb
FEATURES             Location/Qualifiers
     source          1..10034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(81..185)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(260..279)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        451..830
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        832..1030
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     985..1005
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1048..1228
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1275..1400
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1893..2126
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2311..2355
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2629..2746
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2827..2946)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(2956..2976)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2989..3681)
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     regulatory      3678..3687
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3714..3745)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3940..3949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3944..3953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4329..4348
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4833..4856
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4872..4895
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    4945..5533
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4998..5018)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5416..5427)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6052..6232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6258..6275)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6264..6488
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6534..6962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6621..6640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6831..6852
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6971..6991)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6976..7305
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7156..7291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7218..7237
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7353..7400
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7419..7793
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7923..8044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7960..7979)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8014..8033
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8093..8109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8093..8109)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8106..8128)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8117..8133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8141..8147,8148..8165,8166..8171))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8186..8207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8324..8341)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8495..9083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8575..8594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9254..10034,1..11,12..80))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9877..9896
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
       61 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
      121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
      181 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
      241 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
      301 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
      361 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
      421 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
      481 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      541 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      601 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      661 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      721 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      781 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      841 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      901 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      961 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     1021 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1081 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1141 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1201 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     1261 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     1321 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     1381 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     1441 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     1501 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     1561 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1621 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1681 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1741 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1801 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1861 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     1921 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     1981 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     2041 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     2101 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     2161 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     2221 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     2281 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     2341 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     2401 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     2461 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     2521 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     2581 tccattcgat tagtgaacgg atctacaaat ggcagtattc atccacaatt ttaaaagaaa
     2641 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2701 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2761 ggacagcaga aattcacttt gaattcacaa aagctgggta ccgggccccc cctcgagtct
     2821 agattagacg ttgatcctgg cgctggcgca agcagcaggg tgtctatcca tgccgctctc
     2881 ctgggcgcag ctcatgggca gggttcctgc ggcctgttcc tccacctctg gagggaagcc
     2941 gtgagaattt cctactacct tacgcttctt ctttggagcc gtgtacaggg gcaaggcgga
     3001 gccggaggcg atggcgtgct cggtcaggtg ccacttctgg ttcttggcgt cgctgcggtc
     3061 ctcgcgggtc agcttgtgct ggatgaagtg ccagtcgggc atcttgcggg gcacggactt
     3121 ggccttgtag acggtgtcga actggcagcg caagcggcca ccgtccttca gcagcaggta
     3181 catgctcacg tcgcccttca agatgccctg cttgggcacg gggatgatct tctcgcagga
     3241 gggctcccag ttgtcggtca tcttcttcat cacggggccg tcggcgggga agttcacgcc
     3301 gtagaacttg gactcgtggt acatgcagtt ctcctccacg ctcacggtga tgtcggcgtt
     3361 gcagatgcac acggcgccgt cctcgaacag aaaggagcgg tcccaggtgt agccagcggg
     3421 gcaggagttc ttgaagtagt ccacgatgtc ctgggggtac tcggtgaaca cgcggttgcc
     3481 gtacatgaag gcggcggaca agatgtcctc ggcgaagggc aaggggccgc cctccaccac
     3541 gcacaggttg atggcctgct tgcccttgaa ggggtagccg atgccctcgc cggtgatcac
     3601 gaacttgtgg ccgtccacgc agccctccat gcggtacttc atggtcatct ccttggtcag
     3661 tccgtgcttg gactgggcca tggtggcgac cggtacagta ccggattgcc aagctggaag
     3721 tcgagcttcc attatatacc ctctagtgtc taagcttatc tgttctccca ttgacgtcaa
     3781 tggggtgtct gaaagacgtc acagtatgac ggccatggga tctggcagct gacgtcactg
     3841 tctggtgcgg atccccccca aagcccccgg ggctgtaatt gcgtccctct cccgctaggg
     3901 ggcagcagcg agccgcccgg ggcggcgcga attcccgcgg ccgccatggc ggccgggagc
     3961 atgcgacgtc gggcccaatc tgcagcacgt gccgcggtgg cggccgcccg gtaggcgcca
     4021 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     4081 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     4141 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     4201 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     4261 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     4321 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgtc gcgctgttct cctcttcctc
     4381 atctccgggc ctttcgacct cacggtgtta gcgctgctag cgccaccatg tcgggacgcg
     4441 gcaagcaagg aggcaaagct ggagccaaag ccaagacacg ctcttctcgt gccggtctcc
     4501 agttccccgt gggccgagtg catcgactgc tccgcaaggg caactatgct gaacgggtag
     4561 gagctggcgc tcctgtgtac ctggcggcgg tgctggaata cctgactgcc gaaatcctgg
     4621 agctggctgg aaacgccgcc cgcgacaaca agaagacccg cattatcccg cgacacttgc
     4681 agctcgccat ccgcaacgac gaagaactga acaaactcct cgggaaggtc acaatcgcac
     4741 aagggggagt gctccctaaa attcaagcag tcctgctccc taagaaaact gaaagccatc
     4801 ataaagctaa gggaaaggga tatctcggaa attggagcca cccacagttt gagaagggag
     4861 tgagtggaaa ctggtcccat ccccagttcg aaaagtaagc tagctctagc tctagaaatt
     4921 cctcgaggga tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     4981 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5041 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5101 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5161 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5221 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5281 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     5341 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5401 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5461 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5521 gccgcctccc cgcatcgata ccgtcgagac ctagaaaaac atggagcaat cacaagtagc
     5581 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     5641 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     5701 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5761 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     5821 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     5881 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     5941 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     6001 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     6061 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6121 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6181 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     6241 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     6301 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     6361 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     6421 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     6481 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     6541 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6601 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6661 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6721 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6781 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6841 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6901 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6961 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7021 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7081 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7141 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7201 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     7261 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     7321 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     7381 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
     7441 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     7501 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     7561 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     7621 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     7681 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     7741 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     7801 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7861 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7921 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7981 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8041 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     8101 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8161 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8221 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8281 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8341 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8401 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8461 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8521 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8581 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8641 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8701 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8761 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8821 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8881 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8941 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9001 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9061 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9121 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9181 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9241 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9301 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9361 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9421 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9481 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9541 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9601 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9661 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9721 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9781 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9841 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9901 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9961 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10021 ttactttcac cagc
//
