LOCUS       sequence-357505-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient transfection plasmid expressing 
            Renilla-luciferase-beta-globin fusion protein with PTC in 
            beta-globin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: pCMV-3XFLAG-renilla-luciferase-beta-globin-(39...
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(832..855)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttcttggca ccttcaacaa tagcattgga aaagaatcct gggtccgatt caataaacat
       61 ttttggtaaa tcatcacttg cacgtagata agcattataa ttcctaacaa tttgtacaac
      121 gtcaggttta ccacctttta ctaacgggat ttcacgaggc catgataatg ttggacgacg
      181 aacttcacct ttctctttga atggttcaag atatgctgca aattcttctg gttctaactt
      241 tctcatgatt tttgatggca acatggtttc cacgaagaag ttattctcca aaaccatttt
      301 ttctccttct tcagatttga tcaacgcaat atcttcttca atatcaggcc attcatccca
      361 tgattcaatc acatctacta cactttcagc gtgaactatt gctttgatct tatcttgatg
      421 ctcatagcta taatgaaatg ccaaacaagc accccaatca tggccgacaa aaatgatctt
      481 ctttggtaaa ttaagaagtt caaaccatgc agtaagatat ttgtaatgat caagtaacct
      541 ataagaacca ttaccagatt tgcctgattt gcccatacca ataaggtctg gtataataca
      601 ccgcgctact ggctcaatat gtggcacaac atgtcgccat aaataagaag aggccgcgtt
      661 accatgtaaa aaaataacag cattttctgc atgtttttct gaatcataat aattaataaa
      721 tgaatcaaga acattcattt gtttacatct ggcccaccac tgcggaccag ttatcatccg
      781 tttcctttgt tctggatcat aaactttcga agttgaattc gcggccgcaa gcttgtcatc
      841 gtcatccttg taatcgatgt catgatcttt ataatc
//
