LOCUS       sequence-357506-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient transfection plasmid expressing 
            Renilla-luciferase-beta-globin fusion protein with PTC in 
            beta-globin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: pCMV-3XFLAG-renilla-luciferase-beta-globin-(39...
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..68
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 tggactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
       61 atgacaagct tgcggccgcg aattcaactt cgaaagttta tgatccagaa caaaggaaac
      121 ggatgataac tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat
      181 ttattaatta ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta
      241 acgcggcctc ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt
      301 gtattatacc agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata
      361 ggttacttga tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga
      421 agatcatttt tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc
      481 atcaagataa gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat
      541 gggatgaatg gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa
      601 aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa
      661 agttagaacc agaagaattt gcag
//
