LOCUS       sequence-357509-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for stable integration of firefly luciferase control 
            reporter at AAVS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: AAVS1-TetOn-3XFLAG-firefly-beta-globin-control...
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    232..313
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(269..288)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     764..782
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 cccccgctgc acaggctgcc ttccagaagg tggtggctgg agtggccact gccctggccc
       61 acaagtatca ctaaaagggt gggcgcgccg acccagcttt cttgttctgc agcatatgtc
      121 tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
      181 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
      241 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
      301 tttttttcac tgccgacgtc aggtggcact tttcggggtt ggggttgcgc cttttccaag
      361 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
      421 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
      481 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
      541 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
      601 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
      661 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
      721 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
      781 agcctccgga gcgcacgtcg gcagtcggct
//
