LOCUS       sequence-357510-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for stable integration of firefly luciferase control 
            reporter at AAVS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: AAVS1-TetOn-3XFLAG-firefly-beta-globin-control...
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    189..207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             367..432
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(514..532)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgcagact ttactcccta tcagtgatag
       61 agaacgtata aggagtttac tccctatcag tgatagagaa cgtatgacca gtttactccc
      121 tatcagtgat agagaacgta tctacagttt actccctatc agtgatagag aacgtatatc
      181 cagtttactc cctatcagtg atagagaacg tataagcttt gcttatgtaa accagggcgc
      241 ctataaaaga gtgctgattt tttgagtaaa cttcaattcc acaacacttt tgtcttatac
      301 caactttccg taccacttcc taccctcgta aagaattcgc ggccgcaacc gtcagaatta
      361 accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
      421 gacgatgaca agcttgcggc cgcgaattca gaagacgcca aaaacataaa gaaaggcccg
      481 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
      541 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
      601 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
      661 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
      721 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgaca
//
