LOCUS       sequence-357512-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for stable integration of Renilla luciferase control 
            reporter at AAVS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: AAVS1-TetOn-3XFLAG-renilla-beta-globin-control...
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    227..308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(264..283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        330..813
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     759..777
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 gctgcacagg ctgccttcca gaaggtggtg gctggagtgg ccactgccct ggcccacaag
       61 tatcactaaa agggtgggcg cgccgaccca gctttcttgt tctgcagcat atgtctagag
      121 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      181 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      241 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      301 ttcactgccg acgtcaggtg gcacttttcg gggttggggt tgcgcctttt ccaaggcagc
      361 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
      421 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
      481 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
      541 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
      601 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
      661 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
      721 tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct
      781 ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tca
//
