LOCUS       sequence-357564-       13551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13551)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 13551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmNot1-K1537SG1562EQ1658A-dsRNAres_W
FEATURES             Location/Qualifiers
     source          1..13551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2585..2587,2588..2590,2591..3301)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2585..2587,2588..2590,2591..3301)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2630..2651)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2891..2910)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3238..3259
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10857..10898
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10908..10925
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10948..10965)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10998..11132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11022..11041)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11076..11095
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(11733..12321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11813..11832)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12492..13283,13284..13352))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13115..13134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13353..13457)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13525..13543
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 catcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2641 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2701 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2761 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2821 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2881 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2941 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3001 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3061 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     3121 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3181 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3241 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3301 gccaagctta attaagatga acgtagagag ccaactgaag acaccgccaa cgcacattcg
     3361 aaatctggtc aagcacgtga acaagcgaaa ttttaatgag agcagcgagc aaataaatca
     3421 gttcgttaaa gagcacggcc tggaagcgga tcgcagcagc ctgcgccacc tgttctccgt
     3481 gatcaacttc agcgatctcg tgccatcagt tacggtccag ctgcaggcga agctcctggg
     3541 catccagttg gagcgtcagc ttcacagttc atcgtttgtg tccaacatct gctacgcttt
     3601 tgaccagttc ttcgccagca accagaagtc cctgaagcct gttgcggtag cggatctcgt
     3661 tggccaggtc gcccgactga ccggcatcaa caaggtttgt gagtgcgtct ttgccttggc
     3721 cttgacccac tcctcgtaca cagaactcaa acattctgcc aggaacaacc taaaaggtag
     3781 cctcagcgaa cttatagact cttacttggg taataaaggc accggcccag ccgacagcgg
     3841 tctccgggag atctccttcg atctactgca gtatttgctg tgctgcctta gcgagtacgt
     3901 acaaccgcag gtggaagcac agtttttgat caagcttcgt gaggagtttc cgcgccaggc
     3961 ggtaccgctg gttctagcgc cgttccttta cggctcgacg acggcgacga ttgcgggagc
     4021 aggtgccagc gaaacggatg cggaggccga agcgacgact aatagcaaca gctccagttt
     4081 cgaagcagat gctttgaacg aggtgggaat cgaggatatt tatgaccatt taagcgagat
     4141 aatattcacc aaccagggca aaaataatat tatggacaca tcttggatta atctaatcct
     4201 tgaaatcggt tatgaattta catcgagcgt tgaagagtgt aagaatcatt tgtgttcccg
     4261 cgagcgagaa cgcgctgagc tccagtccaa agatgtcgcc aagatcgtgg gtcttatgtg
     4321 ccgacggcac tcctccctgc tcgattgtaa tgtgaatcta ccgacgccgg cgaacttctg
     4381 gcctggtcag ggacagggcg gtggcagcaa cagcagtggc tcttcgcaga cacaaatcac
     4441 tccgcagcaa cagaacccgg gcagcagtaa caacaacgat ggcagcgatg gcaattcgtc
     4501 cagtgataag aaggacaaga aggagacgac ggaggccacg caaacttgga agccagatgt
     4561 ctttgtgcag gcgctcaagg aggtggtgcc tcagctcaac tggaaggacg tgtgcatgga
     4621 acttgatcat cccgagttcg tgctgaagga tcgcatcggt ttggagcttt tgctcaccat
     4681 ccttcggctg gccactggct ctaacatatt tccacatcca gaatgcattt accgtcactg
     4741 ggcaaacacg gaaggtcagc tgtcgcttat cgcgacaatg ctgaagaacc cggatctctt
     4801 ttccttcgcc gactttgtat ttagccagcc agcgttggat gtgcttaaga cagctccgga
     4861 tgcggacaat aaggagatct cgtcctggaa gtcgcttcac ctggtggagg tgctgctctc
     4921 cattgcggac aagggatact atacgcaggt ccatgagcta ttcaagttcc cagcgcagaa
     4981 ctgtcccgat gtgctatttt tggccttatt gaacacaagt ccgcctatga cgccactgcg
     5041 tcaggatctg tttaaccaac tgataccaac gttccttggc aaccatccca actcgaacgt
     5101 gatcttggcc agcgcctgga gctcgaacag cttccaactt cgttccaata tcatgaatgc
     5161 tatgtccgag tggtatctcc gaggcaatga gttcgatcag gtgaaactgt cgcgcatcct
     5221 ggatcttgcc caggatttaa aagcgctatc agcgctgctc aacgcccgtt catttttatt
     5281 cattatcgac ctggcttgcc ttgcctctcg gcgcgagtac ctgaagctgg aaaagtggct
     5341 taccgataaa attcgcgaac atggagagcc ttttatgcag gctataatca aggttctgca
     5401 ccgccgctgc ccgcaggtta tcaacgccaa agtgccagag gatcagctgc ctcccaagca
     5461 ggcgcagctt ttgcccgaga cagttacaac catgatcaac tgtctgcaaa cgtgcatcaa
     5521 taactgcatg cagccggaga tggtcgaagt catcatgcaa atgaccgcga acgtggcgat
     5581 tatggctaac atggcgcgtg cccaacagca gcagcagcca ggattggttc cgccgcctcc
     5641 accaacaatt ctgcgcggcc atcgtggtat ggatctgcct ggaggcatag tccctccgcc
     5701 accgcagcaa ccgttctccg gaaacctcaa cgcgcagatg tttggaccag gaatggatcc
     5761 gctaacgaat atgtccaaca acttagccgg cctcaacctc agcggcccga acggagcatt
     5821 taattttgga aacatgctaa cttctccatc gcgtctgatg actccgggag ccaatcccta
     5881 tccgctgaac ctcatgcaga tgccgcaggc tccgccacca cccaacgtag gaaacctagg
     5941 ccgcatgctg ccagggggtc cacaacagca gactccaacg ccgactccaa cggcccccaa
     6001 tccaaacaac cctgtgatgg ccgatctcca gatacctgtg tcaaaggaag ttgaagacga
     6061 ggtcaactca tactttcagc gcatctacaa ccaccagccg aatccaacgc tgtccatcga
     6121 tgaagtgctg gacattttgc aaagatttaa agagtcaagt aaccggcgcg agcaggaagt
     6181 ctttttgtgc atgctgcgca atctgttcga ggaatatcgc tttttctgtc aatacccaga
     6241 gaaagagcta cagattactg cgcagctatt tggtggcatt attgatcgta acctggtgcc
     6301 cacttttgtt gcactgggcc tttctctgcg ctgtgtcttg gatgccctcc gcaagcccga
     6361 tggatctaag ctttactatt tcggtgtgac ggcactggac agattcagaa ctcgattgca
     6421 cacctacaac aaatactgcg agcatatccg ctccattcct cacttctctg acttcccgcc
     6481 gcaccttatt cagtatgtgg aatacggaat gcacggccag gagccgccgc cgcagaagtt
     6541 gattggactc agcaatacga ttccttcggc gatatctagc ggaccaggaa cggaaccaat
     6601 ttacaggaat agctccatgt taggtaatat gcccgctgcc accccgggat cgggaccgaa
     6661 atcaaacgct gctgtatcgc atgccacgag aatgaagtcc atcgccaatg ccaccaatat
     6721 cgacacactt ttggtggcca accaggaaga gaaagtgact gtgccaccag agcctgtgca
     6781 ggacaaaact gcctttattt tcaacaactt gagccagctg aacataccgc agaagtgcga
     6841 tgagataaag gagattatga ccaaggagta ttggccgtgg ctagcgcaat atctggtcct
     6901 caaacgcgca tcaatggagt tcaacttcca cacactatat tacaactttt tggatgccct
     6961 taaaaacggc gagatcaacc gattcgtaac caaagagacc ctacgcaaca tcaaggtact
     7021 cttgcgctcc gataagggag ttattaattt ctccgatcga agtctgttga agaacctcgg
     7081 acactggttg ggcatgatga ccttaggtcg caatcgcccc atcctgcagt tggatctgga
     7141 tcttaaatcc cttttggctg aggcttacca caagggccag caggaactgc tttttgtggt
     7201 tcctttcgta gccaagatcc ttgagtcatc cgccaagtct cgcatcttcc gatcgcccaa
     7261 tccatggaca atgggtatca tgtacgtgct tgctgagctt caccaggagc cagaccttaa
     7321 gctgaacctg aagtttgaaa tcgaagtact ctgcaaaaca cttaatctgg agttggccaa
     7381 gttgcgacaa gtgatctacc tgaaggatcc caatcgaact caccggattg aagagcagat
     7441 gtcgcaacca aaaccgaaac aacttgagcc agtggcttct gcgccagctc taccgcgtga
     7501 acagcaatcc ccggcacaac ctccgccacc accacaacag cagcaaccac cgcagcagca
     7561 ggtacctcca ccgccatcct ctgcggacgt ggacgcccaa aatgcggccg ctatgatgat
     7621 ggcagcgggc ggagctaata gtactcctgg atcggtatct tcgcccaatc tacccaccga
     7681 ttccagccag gtggcactac cgccaccaga gccgcgctat tcatacgtgg acgtaaacgt
     7741 gagcaacttc cagcttattg gtcagcagtt ggtactgcca cccaatactc cgttcctgca
     7801 cgccaatccc ggaatcaaac acatggtcgt caacgccgtg gagcgcacga taaccgactg
     7861 gttgcagcct attgtggacc gaagcatccg cattgcctgt gccaccaccg agcagatcat
     7921 ccgcagcgac ttcgccctgg atgctgacga gaaccgaatg cgcactgccg ctcatcagat
     7981 ggtacgaaac cttgccgccg agatggccat gattaccggt aaggatgaga tagcgcgtgc
     8041 tattagccag aatctgcaca aggccttgct gtcgggtcta aatgggatgc ccagcatggc
     8101 tgagatccaa gctgctgcta tgcagttagc cagcgaaaat gttgagctag tttgcgcttt
     8161 cattcagaaa acatctgctg aaaaggcagc cgcagagatc gataggcgtc taagcaccga
     8221 tttcgagacc agaaaaattg ctcgtgaaga gggcaaccgc tttgtggacg cccagatcct
     8281 cacctatgca caggagcgtc ttccagaagc agtgcgcatc aaggtgggcg cggctccagc
     8341 tacactttat gctgtgtact cggagttcgc tagaagcata cctggcttcc agcagatgag
     8401 cgatcgcgat attgccctgt ttgtgcccaa gccgacggat ttgtctcagc caaatgtgtt
     8461 tgctaatgat gatagcatgg tatacgggga actggctagc aaaatggagg ccttcatgaa
     8521 cacggcaatc ggagtgccga cactccagat ccaggctagc aagatgcaca tgctactcaa
     8581 cgccctgata gcgacgcgtc gactgcgtga ccaagagtct gcctttaatc tgctgacccg
     8641 cgcggtcgag ggtttgaccg aaggattggt caatatgcat gaaaacatgg agcaaatgaa
     8701 gatgtaccaa aacatccatc tccgtatcct tggcttgctg aacaacagtt tcggtgctcc
     8761 aaacacagag cgagcggtaa caaagtgttt cttcgatatc cgagaggagg tgcgatataa
     8821 cgtggaggca gctcgcgctt tgattacgtc gcactttgtc aatctgaatc agtttgacgg
     8881 aatgctacgc gactgcatgg acaacggaaa caattatgtg gctatatcgt tcggtatcgc
     8941 actgctggag cgcctcatca tggacgatcg agtgattaat attgtttcgg acaacgaatt
     9001 catggcaact gttgagctgc taggtaggct gacccaacac cgtcaccgct accccgagtg
     9061 tatcgtcaac gccatcgaca ccctgtggtc cggcaatttc aatagctcct ccgattattc
     9121 cccctttaat ggaaacgacc gctatctgag cggcgctagc cactatattc atagcggaat
     9181 gcaccatgtc cgctcgtgtg acaccgacga cccccccgga ctccaggaaa aaaccgaatt
     9241 cttgttgaaa gactgggtgg ccttgtatac ccagcaaaat caacaatcga cccgtgacgc
     9301 ccgtaatttt ggcgccttcg tgcaaaaaat gaatacctac ggcattttga agaccgatga
     9361 tctgattacg cgcttctttc gtcaagctac ccatatctgt accgacgtcg tctatcgtat
     9421 gtttgctgaa cccagccttc ccattaatca agctaagaat aaaatttttc aatggattga
     9481 cgcctttgtg cacttgatcg ccatgctggt ccgccattcg ggagaggccg gcaaccccac
     9541 gacgaaaatt aatttgctca ataaagtctt gggaatcgtc ttgggcacct tgatcaaaga
     9601 tcacgaaatg cgcggagtgt cctttcaaca agtgggatat caccgttttt ttatgatgtt
     9661 gtttatggaa ttgtgtaccg ctgacgtcat cctggaaagc ttgatgcata gcatcgtcag
     9721 cgcttttgct tacacatatc acttgttgaa tcccagcgtg gctccgggat tctgctttgc
     9781 ttggttggag ctcatttcgc atcgcgtctt cctgggccgc atcctggtcc aaattcccgg
     9841 gcaaaagggc tggccacttt acgctcaatt gctgcaggac ctttttaaat atcttgcgcc
     9901 cttcttacgc aacactgagc tcggcaaacc agtccagctt ttgtacaagg ggacgctacg
     9961 tgttctgctc gttctgcttc acgacttccc ggagttcttg tgcgactatc attttgggtt
    10021 ctgtgacacc atcccgccca attgcgtcca gatgcgtaac atcatcctgt cggcgtttcc
    10081 gcgcaacatg cgcttgcccg atcccttcac gcccaaccta aaagtggaca tgctgtcgga
    10141 cagtagtaat gcgcctaagg tgctcagtag ctacattatg aacatccagc ccgcgaactt
    10201 taagaaggat ttggactcgt acctcaaggc ccgggcgcca gttacttttc tatcggaatt
    10261 gcgtggtcac ttgcaggtga caagcgagcc aggaactcgt tacaacatgg cgctgatgaa
    10321 tgctctggtc atgtatgtgg gaactcaggc gattgctttg atcagaaaca aaaactttgt
    10381 gcccaacacc tcgaacatag cacacagcgc ccacatggat atcttccaga atcttgctgt
    10441 tgacttggat acggagggtc gctatctgtt cttgaatgcg attgcgaacc agttgcgcta
    10501 tcccaacagc cacacgcact acttcagctg tgcggtgctg catctgtttg ccgaggccaa
    10561 ctcggaggca attcaggagc agatcactcg cgtacttctg gaacgattga tcgtgaaccg
    10621 cccgcatcct tggggactgt tgatcacttt tattgagctg atcaaaaatc ccatctacaa
    10681 gttctgggac catgactttg tgcactgtgc gccagagatc accaagctct ttgaatcggt
    10741 ggcccgttcc tgtctggcca agtcgaacgt cacccagcag ttgaacatgc ccgtcgtcga
    10801 tggcgagggc caggaggtag ccaccatcaa ctgatctaga gggcccgcgg ttcgaaggta
    10861 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc
    10921 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct
    10981 gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca
    11041 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
    11101 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
    11161 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
    11221 gtatggctga ttatgatcag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
    11281 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
    11341 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
    11401 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
    11461 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
    11521 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
    11581 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
    11641 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggcca gcaaaaggcc
    11701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    11761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    11821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    11881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    11941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    12001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    12061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    12121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
    12181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    12241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    12301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    12361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    12421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    12481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    12541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    12601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    12661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    12721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    12781 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
    12841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    12901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    12961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    13021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    13081 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
    13141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    13201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    13261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    13321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    13381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13441 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
    13501 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg t
//
