LOCUS       sequence-357571-       10032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmids for production of LV-based TFactivity reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Abe H, Abe K
  TITLE     PCR-based profiling of transcription factor activity in vivo by a 
            virus-based reporter battery.
  JOURNAL   iScience. 2022 Feb 14;25(3):103927. doi: 10.1016/j.isci.2022.103927.
            eCollection 2022 Mar 18.
  PUBMED    35281741
REFERENCE   2  (bases 1 to 10032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-14"; volume: "25(3):103927. doi"; pages: " 
            10.1016/j.isci.2022.103927. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pLVTFrep4-creb
FEATURES             Location/Qualifiers
     source          1..10032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2413..2530
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2611..2730)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(2740..2760)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3447..3456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3483..3514)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3709..3718
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3713..3722
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4098..4117
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4606..4635
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4651..4680
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    4726..5314
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4779..4799)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5197..5208)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5833..6013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6039..6056)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6045..6269
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6315..6743
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6402..6421)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6612..6633
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6752..6772)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6757..7087
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6938..7073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7000..7019
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7135..7182
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7201..7575
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7705..7826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7742..7761)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7796..7815
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7875..7891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7875..7891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7888..7910)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7899..7915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7923..7929,7930..7947,7948..7953))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7968..7989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8106..8123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8277..8865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8357..8376)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9036..9827,9828..9896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9659..9678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9897..10001)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctac aaatggcagt
     2401 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2461 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2521 tcaaaatttt cgggtttatt acagggacag cagaaattca ctttgaattc acaaaagctg
     2581 ggtaccgggc cccccctcga gtctagatta gacgttgatc ctggcgctgg cgcaagcagc
     2641 agggtgtcta tccatgccgc tctcctgggc gcagctcatg ggcagggttc ctgcggcctg
     2701 ttcctccacc tctggaggga agccgtgaga atttcctact accttacgct tcttctttgg
     2761 agccgtgtac accagaaaca gatggtggcg tccctcggtt ctctcgtact gctccacaat
     2821 ggtataatcc tcgttatggg aggtgatatc cagctttgtg tccacatagt agtatccggg
     2881 gagctgcacg ggtttctttg ccatgtagat agatttgaac tccaccaggt aatggccgcc
     2941 atccttcagt ttcagggctt tgtgaatttc gcccttcagc acgccatctc tggggtacag
     3001 tctctcggtg gaggcttccc atcccatggt ctttttctgc atcactggtc catcggaggg
     3061 aaaattcact ccaataaatt tcactttgta aatgaagcat ccgtcctgga gggaggaatc
     3121 ctgtgtcacg gtcgccactc ctccatcttc aaaattcatc actctttccc acttaaagcc
     3181 ctcagggaag gacagctttt tatagtcggg aatatcggcg ggatgcttca catacacctt
     3241 ggagccgtac tggaactgag gggacagaat atcccaggcg aagggcagag ggccgccttt
     3301 ggtcactttc agcttcacgg tgttatggcc ctcatagggg cgtccctctc cttcgccctc
     3361 aatttcgaac tcatgtccat tcactgtgcc ttccatgcgc accttgaatc tcataaattc
     3421 cttgatcacg ttcttggagg acctcaccat ggtggcgacc ggtacagtac cggattgcca
     3481 agctggaagt cgagcttcca ttatataccc tctagtgtct aagcttatct gttctcccat
     3541 tgacgtcaat ggggtgtctg aaagacgtca cagtatgacg gccatgggat ctggcagctg
     3601 acgtcactgt ctggtgcgga tcccccccaa agcccccggg gctgtaattg cgtccctctc
     3661 ccgctagggg gcagcagcga gccgcccggg gcggcgcgaa ttcccgcggc cgccatggcg
     3721 gccgggagca tgcgacgtcg ggcccaatct gcagcacgtg ccgcggtggc ggccgcccgg
     3781 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     3841 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     3901 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     3961 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     4021 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     4081 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgtcg cgctgttctc
     4141 ctcttcctca tctccgggcc tttcgacctc acggtgttag cgctaccggt cgccaccatg
     4201 agtggccgcg gaaaacaggg cggaaaggct cgggcaaagg caaaaactcg gtccagccgg
     4261 gctggcctcc agtttccagt cggcagggtc caccggctgc tgcggaaagg aaattacgct
     4321 gagcgggtcg gggctggcgc gccggtgtac ctggccgccg tcctggaata tctgaccgct
     4381 gaaattctgg aactggcggg caacgccgcc cgcgacaaca agaagacccg catcattcca
     4441 cggcatttcc agctcgccat caggaacgac gaagaactga ataagctgct gggcaaagta
     4501 acaattgccc aaggcggagt gctgccaaat attcaagccg tcttgctgcc taaaaagact
     4561 gaatcccacc acaaggccaa aggcaagggc tatctgggcg gaggcgagca gaaactgatt
     4621 tctgaagaag acctcgctgt aggttcaggt gaacaaaagc tcatcagcga ggaggacctg
     4681 gctagctagc tctagaaaat tcctcgaggg atcaagctta tcgataatca acctctggat
     4741 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4801 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4861 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     4921 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4981 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5041 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5101 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     5161 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5221 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5281 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgaga cctggaaaaa
     5341 catggagcaa tcacaagtag caacacagca gctaccaatg ctgcttgtgc ctggctagaa
     5401 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     5461 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5521 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     5581 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     5641 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     5701 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     5761 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     5821 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5881 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5941 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6001 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     6061 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6121 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6181 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     6241 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     6301 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6361 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6421 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt
     6481 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6541 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6601 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     6661 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat
     6721 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     6781 tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6841 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6901 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6961 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7021 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7081 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     7141 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     7201 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     7261 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     7321 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     7381 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     7441 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     7501 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     7561 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     7621 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     7681 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     7741 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7801 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     7861 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7921 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7981 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8041 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8101 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8161 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8221 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8281 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8341 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8401 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8461 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8521 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8581 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8641 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8701 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8761 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8821 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8881 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8941 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9001 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9061 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9121 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9181 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9241 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9301 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9361 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9421 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9481 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9541 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9601 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9661 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9721 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9781 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9841 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9901 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9961 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10021 ccacctgacg tc
//
