LOCUS       sequence-357578-        7904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human HNRNPAB 3` UTR region containing 2x binding sites for miR-150 
            cloned downstream of luciferase reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Chirichella M, Bianchi N, Dzafo E, Foli E, Gualdrini F, Kenyon A, 
            Natoli G, Monticelli S
  TITLE     RFX transcription factors control a miR-150/PDAP1 axis that 
            restrains the proliferation of human T cells.
  JOURNAL   PLoS Biol. 2022 Feb 10;20(2):e3001538. doi: 
            10.1371/journal.pbio.3001538.
  PUBMED    35143476
REFERENCE   2  (bases 1 to 7904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2022-02-10"; volume: "20(2):e3001538. doi"; pages: " 
            10.1371/journal.pbio.3001538"
COMMENT     Sequence Label: pmiRGLO Dual-Luciferase-HNRNPAB-3'UTR
FEATURES             Location/Qualifiers
     source          1..7904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     333..352
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      441..450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             447..2099
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2821..2942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2832..2851)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2886..2905
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3187..3544
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3187..3378
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3210..3230)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3395..3530
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      3589..3598
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3595..4527
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             4579..5370
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4630..4649)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5240..5259
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5434..5482
                     /note="synthetic polyadenylation signal"
     promoter        5638..5742
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5743..5811,5812..6603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5961..5980)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6761..7349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6841..6860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    7465..7748
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        join(7805..7904,1..400)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7840..7863)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
       61 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
      121 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
      181 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
      241 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
      301 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
      361 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttggc
      421 aatccggtac tgttggtaaa gccaccatgg aagatgccaa aaacattaag aagggcccag
      481 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc
      541 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta
      601 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc
      661 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg
      721 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc
      781 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag
      841 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca
      901 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt
      961 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca
     1021 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc
     1081 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca
     1141 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca
     1201 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc
     1261 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat
     1321 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga
     1381 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc
     1441 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca
     1501 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta
     1561 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg
     1621 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca
     1681 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact
     1741 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag
     1801 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc
     1861 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa
     1921 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc
     1981 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg
     2041 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat
     2101 tctagttgtt taaacgagct cgctagcggc ggcagcagga gcgaccaact gatcgcacac
     2161 atgctttgtt tggatatgga gtgaacacaa ttatgtacca aatttaactt ggcaaacttt
     2221 ctattgcctg tcccatgtgc atcttattta aaatttcccc catggaaatc actctcctgt
     2281 tgactatttc cagagctcta ggtgtttagg cagcgtgtgg tgtctgagag gccatagcgc
     2341 catcatgggc tgatttttat taccaggtcc cccagaagca ggtgggaggc tctgcttcct
     2401 gctgccgctc tgcagcctgg acctgtggac cctggttgta aagagtaaat tgtatcttag
     2461 gaaaccagtg tcaccttttt ttcacctttt aattttatat tatttgcgtc atacatttcc
     2521 tgtaacggaa gtgttaattt tactgtactt tttggtacct tttgggaatc taatgtattg
     2581 taaggtattt tacacgtgtc ctgattttgc cacaacctgg atattgaagc tatccaagct
     2641 tttgaaataa aatttaaaaa cccccaagcc tgggtgagtg tgggatatct agagtcgacc
     2701 tgcaggcatg caagctgatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     2761 tgccaccgct gagcaataac tagcataacc ccttggggcg gccgcttcga gcagacatga
     2821 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     2881 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2941 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
     3001 ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatcga attttaacaa aatattaacg
     3061 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3121 tacgcggatc tgcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg
     3181 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3301 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3361 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3421 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3481 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3541 caaaaagctt gattcttctg acacaacagt ctcgaaccaa aggctggagc caccatggct
     3601 tccaaggtgt acgaccccga gcaacgcaaa cgcatgatca ctgggcctca gtggtgggct
     3661 cgctgcaagc aaatgaacgt gctggactcc ttcatcaact actatgattc cgagaagcac
     3721 gccgagaacg ccgtgatttt tctgcatggt aacgctgcct ccagctacct gtggaggcac
     3781 gtcgtgcctc acatcgagcc cgtggctaga tgcatcatcc ctgatctgat cggaatgggt
     3841 aagtccggca agagcgggaa tggctcatat cgcctcctgg atcactacaa gtacctcacc
     3901 gcttggttcg agctgctgaa ccttccaaag aaaatcatct ttgtgggcca cgactggggg
     3961 gcttgtctgg cctttcacta ctcctacgag caccaagaca agatcaaggc catcgtccat
     4021 gctgagagtg tcgtggacgt gatcgagtcc tgggacgagt ggcctgacat cgaggaggat
     4081 atcgccctga tcaagagcga agagggcgag aaaatggtgc ttgagaataa cttcttcgtc
     4141 gagaccatgc tcccaagcaa gatcatgcgg aaactggagc ctgaggagtt cgctgcctac
     4201 ctggagccat tcaaggagaa gggcgaggtt agacggccta ccctctcctg gcctcgcgag
     4261 atccctctcg ttaagggagg caagcccgac gtcgtccaga ttgtccgcaa ctacaacgcc
     4321 taccttcggg ccagcgacga tctgcctaag atgttcatcg agtccgaccc tgggttcttt
     4381 tccaacgcta ttgtcgaggg agctaagaag ttccctaaca ccgagttcgt gaaggtgaag
     4441 ggcctccact tcagccagga ggacgctcca gatgaaatgg gtaagtacat caagagcttc
     4501 gtggagcgcg tgctgaagaa cgagcagacc ggtggtggga gcggaggtgg cggatcaggt
     4561 ggcggaggct ccggagggat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4621 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     4681 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     4741 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     4801 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4861 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     4921 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     4981 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5041 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5101 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5161 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5221 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5281 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5341 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     5401 aagcgacgcc caacctgcca tcacgatggc cgcaataaaa tatctttatt ttcattacat
     5461 ctgtgtgttg gttttttgtg tgaatcgata gcgataagga tcctctttgc gcttgcgttt
     5521 tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga
     5581 ctggctttct acgtaatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5641 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5701 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5761 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5821 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5881 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5941 atgagcactt tcaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6001 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6061 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6121 atgagtgata acactgcggc caacttactt ctgacaacta tcggaggacc gaaggagcta
     6181 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6241 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6301 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6361 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6421 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6481 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6541 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6601 taattcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca aaggcggtaa
     6661 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6721 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6781 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6841 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6901 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6961 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7021 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7081 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7141 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7201 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7261 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7321 agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg
     7381 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7441 tcttcaccta gatcctttta tagtccggaa atacaggaac gcacgctgga tggcccttcg
     7501 ctgggatggt gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc
     7561 ctgtaccctc tggttgcata ggtattcata cggttaaaat ttatcaggcg cgattgcggc
     7621 agtttttcgg gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc
     7681 gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta
     7741 gccatcgaga taaaccgcag tactccggcc acgatgcgtc cggcgtagag gatcgagatc
     7801 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     7861 ctgggcactt ggcgctacac aagtggcctc tggcctcgca caca
//
