LOCUS       sequence-357589-        9676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  wrmScarlet11^SEC^3xMyc vector with ccdB sites for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Witten G, DeMott E, Huang G, Zelasko F, de Jesus B, Mulchand C, 
            Schuck L, Pullman S, Perez A, Mahableshwarkar P, Wu Z, Cardona EA, 
            Pierce JT, Dickinson DJ, Doonan R
  TITLE     mScarlet and split fluorophore mScarlet resources for plasmid-based 
            CRISPR/Cas9 knock-in in C. elegans.
  JOURNAL   MicroPubl Biol. 2023 Jun 14;2023:10.17912/micropub.biology.000871. 
            doi: 10.17912/micropub.biology.000871. eCollection 2023.
  PUBMED    37396790
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2023-06-14"; volume: 
            "2023:10.17912/micropub.biology.000871. doi"; pages: " 
            10.17912/micropub.biology.000871. eCollection 2023"
COMMENT     Sequence Label: pGLOW63
FEATURES             Location/Qualifiers
     source          1..9676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1095..1115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1133..1166)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             1176..1202
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      complement(3020..3343)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5261..5285
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5288..6313
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5288..6313
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6314..6338)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6691..6724)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             6743..6832
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             complement(6877..7182)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7455..7471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7455..7471)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7468..7490)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7479..7495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7503..7509,7510..7527,7528..7533))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7548..7569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7686..7703)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7857..8445)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7937..7956)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8616..9407,9408..9476))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9239..9258
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9477..9581)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9649..9667
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg tacaccgtcg tcgagcaata cgagaagtcc gtcgcccgtc
     1081 actgcaccgg aggagagaat ctgtactttc aatccggaaa ggtaagttta aaataacttc
     1141 gtataggata ctttatacga agttattttc agggataccc atacgacgtc ccagactacg
     1201 cctaaggatg atcgacgcca acgtcgttga attttcaaat tttaaatact gaatatttgt
     1261 tttttttcct attatttatt tattctcttt gtgttttttt tcttgctttc taaaaaatta
     1321 attcaatcca aatctaaaca tttttttttc tctttccgtc tcccaattcg tattccgctc
     1381 ctctcatctg aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg
     1441 tggggggaat tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca
     1501 cacaatatgc aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa
     1561 aatatataga ggaggaagga aaactagctg ccctcgattg tctatcttcc attctcacca
     1621 agtttcaaca actttattca gtcattcttc tctggttcat ttcccaacca atcttcccga
     1681 ataaagccca tctgtccttt cggccacatt cctcacctac atccgtactc cttatctccc
     1741 gttcttatcc agtcttttat cgtctctgcg tctcgcacac tgtctcgtcg gtaatgtaaa
     1801 aaaaaaacaa aaaaaacttg aaaacttgcg gaaagatttc ctaataaaag gagacgaatc
     1861 cggtaggaaa tcgacaagtc tctgcttctc gaagactagg tgagttacat ttctttggcc
     1921 gaattatttt gaaatcctaa atttgataat ttcagagatg tctgtaaaac ttgcgtgtta
     1981 tgtgacggct tcggtcactg tcgccactct tatggtatgc ttcatgacca tgtcaaccat
     2041 ctactcggag gttgatggat tcagagagaa gcttgatacc gagatgaatg tgttcagagt
     2101 gagttaaatt atattttgaa ttttaaataa tttttaattt tctagcaatc taccaatgga
     2161 ttgtggaagg acatagttgt catcggaaga tctagcaagc gtgtccgttg tcaatatgaa
     2221 gagaccaacg ctaccccaac tccacatgct gatggatccc catctgctcc accaggtcaa
     2281 ccaccagcag ttccaccagt cttcaaccag ccaaagactc caaatggagc caatggaaat
     2341 ggaccaacct gcaactgcaa tgctgataac aagtgcccag ctggaccatc cggaccaaag
     2401 ggagttccag gagttccagg actcgacgga gttccaggac ttgacggtgt tccaggagtt
     2461 ggagctgatg atatcgctcc acaacgcgag tctgtcggat gcttcacttg cccacaagga
     2521 ccagttggac caccaggagc tcttggaaga ccaggaccac gtggacttcc aggaccaaga
     2581 ggacaaaatg gaaacccagg aagagatgga caaccaggac atccaggaga gcaaggatca
     2641 tccggtcaaa tcggaaagat cggagagcca ggaccaccag gagagaaggg acgcgacgcc
     2701 gagcatccaa tcggaagacc aggaccaaag ggaccaagag gagatcaagg accaacagga
     2761 ccagctggac agaacggtct tcacggacca ccaggagagc caggaaccgt tggaccagaa
     2821 ggaccatctg gaaagcaagg acgtcaagga ccagacggaa cccagggaga gactggacca
     2881 gacggaagac caggaaagga tgccgagtac tgccagtgcc cagacaagtc tccaccatca
     2941 gaggctgtca acgccaaccg tggatacaga aatatctaaa ttgttggtgt tttctaataa
     3001 aatatttgag attcagtgag acttttttct tggcggcaca ataaagtttc ctctttttgt
     3061 tgggtcactc aacgaacaca atacaaatat ttgagcgctc gcatttttct caacctttcg
     3121 ggtttaaagc acttcaaaat ggaattgaag agagattgtg aaagtacatc agacagaatc
     3181 acattttgat agcattcact tcactcagat gcaaactttt cagtgactat aaaaagtcat
     3241 aaaataataa cattaaaaaa gcaaataaat tagcgagaga attttttgac aaaaagaaag
     3301 aagagtgata gagaagaagg gaatgcttga aaggattttg catttatctt aatcaccgtc
     3361 ttcgagaaga cgaaccatgg cgccagtttc agaatccaag tttctaatat agttcatcac
     3421 aatgttgacg ttggtccaac ctccggcttg cataatctct gggatggaaa ctccagcgcg
     3481 agccatgtca cgggcagctc cgacacgggc ggaatgtcca gaccaagcca agtaacgttg
     3541 tccggaatca tccttggctc cgtagatgag acggtgggta gcttcgaaga ttcctgaaaa
     3601 tttattttca gcttattctt agctaatatg ttgtttaaac ttaccttcaa gagctcgagt
     3661 cgagagttgt gatgttgcac tcggggcagc aactccgttc ttgcgaacac gacagaacag
     3721 gtagttgttt ggatcatcgg cgactccgga aaccgaaatc cagcgctcaa cgagcttggt
     3781 aactccaagg gagagcgctt tttcgactcc ggcagtcgag accagcgttt ttgtacgtcc
     3841 gatgtggatg agcattcttc ctccgtctgt gcgactaata tccttgacgc gaatgcgggc
     3901 aatttcggcg atacggagaa gtgtgttgta agcaattccg aggaaggcga ggtttcggat
     3961 gtcctggcaa cgatcggaat tttccataag agaacggacc tgatcgaagt cggttctgaa
     4021 aatttcgtca agtcttagga ttgaatacat taatatttaa acttaccttt cgaaggcgag
     4081 ggcttgcttg gctctctctc cagcatcaac attttccttg cggatacgtc tcattacgag
     4141 agatacggca ttagaatctg atgggcgtgg aagtccagat cggcgatgga gcatattgag
     4201 ctgtccgaga tgttgttgga tcgtcttcac cgccaatcca cgagcttgga ggtagagcaa
     4261 gtagtcacga acgtcttctg gctcggctgg gaaccacttg cggttgttaa gcttgcacca
     4321 ggcagcccaa gaacggcaca ctgagagaag catcttccag gtgtgctcgg agaaggcttg
     4381 tctgtcacgg aacatatcca tgaggttctt gcgaacttca tcacttgtcg cgtcaactgg
     4441 aagtgctgga agattttgat gaacagtgag aagatttgac atattttcga agttttttag
     4501 atgcactaga acaaagcgtg ttggcttcct ctgagcccgc tttccttata tacccgcatt
     4561 ctgcagcctt acagaatgtt ctagaaggtc ctagatgcat tcgtttgaaa atactcccgg
     4621 tgggtgcaaa gagacgcaga cggaaaatgt atctgggtct ctttattgtg tacactactt
     4681 ttccatgtac cgaatgtgag tcgccctcct tttgcaacaa gcagctcgaa tgttctagaa
     4741 aaaggtggaa aatagtataa ataccgttga aaataaatac cgaacaacat ttgctctaat
     4801 tgtgaaatta gaaatcttca aactataatc atgtcacttt accactattt ccgtccagct
     4861 caacgttccg tttttggtga tcatttttgc tttcgtcgta aatctacaca cgcgtctctt
     4921 ccgtgcgaga gtccaagcca gcagccaaat tcgttgactg agtattcaac gtttatacgt
     4981 tgtcggcaac gagaaatagg aaaatgcatc gggaaatgtt cttttttcga ttttttccaa
     5041 ggttttgaca aattttacca cgaattttgc tatgttttca attaaaaaat atgttattca
     5101 actgtttcta tgaggaaaat aaggctttgc atgtaatttt cttattcagc ataattttta
     5161 attaatttga attttctgtc ctaacgttta ttttgttttc ttggttatga ctgatctgaa
     5221 attaattttt gaattttaag gtaatatgtc aggcggtgcc gcaagtttgt acaaaaaagc
     5281 aggctccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     5341 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     5401 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     5461 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     5521 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     5581 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     5641 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     5701 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     5761 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     5821 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     5881 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     5941 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     6001 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     6061 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     6121 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     6181 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     6241 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     6301 ggcaaaggaa tagacccagc tttcttgtac aaagtgggtc caattactct tcaacatccc
     6361 tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc
     6421 ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt
     6481 caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta
     6541 ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact
     6601 tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
     6661 ttacgtttca gtttatgacc gcaattttta ataacttcgt ataggatact ttatacgaag
     6721 ttattttcag ggagccggat ctgaacaaaa acttatttct gaagaggatc ttgagcaaaa
     6781 gctcatctcc gaggaggacc tcgagcagaa gttgatcagc gaggaagact tgccggctcg
     6841 acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt
     6901 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
     6961 ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
     7021 caccaccggg taaagttcac gggggacttt atctgacagc agacgtgcac tggccagggg
     7081 gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
     7141 ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct
     7201 cgtcggcaaa agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat
     7261 tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta
     7321 ccgaaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac
     7381 tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg ggttcctagg
     7441 aaatcgataa catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7501 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7561 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7621 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7681 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7741 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7801 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7861 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7921 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7981 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8041 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8101 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8161 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8221 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8281 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8341 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8401 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8461 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8521 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8581 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8641 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8701 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8761 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8821 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8881 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8941 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9001 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9061 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9121 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9181 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9241 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9301 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9361 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9421 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9481 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9541 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9601 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9661 acgaggccct ttcgtc
//
