LOCUS       sequence-357596-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for stable integration of firefly luciferase NMD(+) 
            PTC39 reporter at AAVS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-A...
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..40
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    58..76
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    94..112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    130..148
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    166..184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    202..220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             380..445
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(527..545)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gagaacgtat gaagagttta ctccctatca gtgatagaga acgtatgcag actttactcc
       61 ctatcagtga tagagaacgt ataaggagtt tactccctat cagtgataga gaacgtatga
      121 ccagtttact ccctatcagt gatagagaac gtatctacag tttactccct atcagtgata
      181 gagaacgtat atccagttta ctccctatca gtgatagaga acgtataagc tttgcttatg
      241 taaaccaggg cgcctataaa agagtgctga ttttttgagt aaacttcaat tccacaacac
      301 ttttgtctta taccaacttt ccgtaccact tcctaccctc gtaaagaatt cgcggccgca
      361 accgtcagaa ttaaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
      421 cgattacaag gatgacgatg acaagcttgc ggccgcgaat tcagaagacg ccaaaaacat
      481 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
      541 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
      601 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
      661 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
      721 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
      781 catttataat gaacgtgaat tgctcaacag
//
