LOCUS       sequence-357597-        3606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for bi-cistronic expression of mCherry and PC 
            (seperated by P2A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Igelmann S, Lessard F, Uchenunu O, Bouchard J, Fernandez-Ruiz A, 
            Rowell MC, Lopes-Paciencia S, Papadopoli D, Fouillen A, Ponce KJ, 
            Huot G, Mignacca L, Benfdil M, Kalegari P, Wahba HM, Pencik J, Vuong
            N, Quenneville J, Guillon J, Bourdeau V, Hulea L, Gagnon E, Kenner 
            L, Moriggl R, Nanci A, Pollak MN, Omichinski JG, Topisirovic I, 
            Ferbeyre G
  TITLE     A hydride transfer complex reprograms NAD metabolism and bypasses 
            senescence.
  JOURNAL   Mol Cell. 2021 Sep 16;81(18):3848-3865.e19. doi: 
            10.1016/j.molcel.2021.08.028.
  PUBMED    34547241
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.08"; journalName: "Mol Cell"; date: 
            "2021-09-16- 16"; volume: "81"; issue: "18"; pages: "3848-3865.e19"
COMMENT     Sequence Label: pUltra-Hot-PC
FEATURES             Location/Qualifiers
     source          1..3606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..57
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gcaacaaact tctcactact caaacaagca ggtgacgtgg aggagaatcc cgggccttct
       61 agaatgctga agttccgaac agtccatggg ggcctgaggc tcctgggaat ccgccgaacc
      121 tccaccgccc ccgctgcctc cccaaatgtc cggcgcctgg agtataagcc catcaagaaa
      181 gtcatggtgg ccaacagagg tgagattgcc atccgtgtgt tccgggcctg cacggagctg
      241 ggcatccgca ccgtagccat ctactctgag caggacacgg gccagatgca ccggcagaaa
      301 gcagatgaag cctatctcat cggccgcggc ctggcccccg tgcaggccta cctgcacatc
      361 ccagacatca tcaaggtggc caaggagaac aacgtagatg cagtgcaccc tggctacggg
      421 ttcctctctg agcgagcgga cttcgcccag gcctgccagg atgcaggggt ccggtttatt
      481 gggccaagcc cagaagtggt ccgcaagatg ggagacaagg tggaggcccg ggccatcgcc
      541 attgctgcgg gtgttcccgt tgtccctggc acagatgccc ccatcacgtc cctgcatgag
      601 gcccacgagt tctccaacac ctacggcttc cccatcatct tcaaggcggc ctatgggggt
      661 ggagggcgtg gcatgagggt ggtgcacagc tacgaggagc tggaggagaa ttacacccgg
      721 gcctactcag aggctctggc cgcctttggg aatggggcgc tgtttgtgga gaagttcatc
      781 gagaagccac ggcacatcga ggtgcagatc ttgggggacc agtatgggaa catcctgcac
      841 ctgtacgagc gagactgctc catccagcgg cggcaccaga aggtggtcga gattgccccc
      901 gccgcccacc tggacccgca gcttcggact cggctcacca gcgactctgt gaaactcgct
      961 aaacaggtgg gctacgagaa cgcaggcacc gtggagttcc tggtggacag gcacggcaag
     1021 cactacttca tcgaggtcaa ctcccgcctg caggtggagc acacggtcac agaggagatc
     1081 accgacgtag acctggtcca tgctcagatc cacgtggctg agggcaggag cctacccgac
     1141 ctgggcctgc ggcaggagaa catccgcatc aacgggtgtg ccatccagtg ccgggtcacc
     1201 accgaggacc ccgcgcgcag cttccagccg gacaccggcc gcattgaggt gttccggagc
     1261 ggagagggca tgggcatccg cctggataat gcttccgcct tccaaggagc cgtcatctcg
     1321 ccccactacg actccctgct ggtcaaagtc attgcccacg gcaaagacca ccccacggcc
     1381 gccaccaaga tgagcagggc ccttgcggag ttccgcgtcc gaggtgtgaa gaccaacatc
     1441 gccttcctgc agaatgtgct caacaaccag cagttcctgg caggcactgt ggacacccag
     1501 ttcatcgacg agaacccaga gctgttccag ctgcggcctg cacagaaccg ggcccaaaag
     1561 ctgttgcact acctcggcca tgtcatggta aacggtccaa ccaccccgat tcccgtcaag
     1621 gccagcccca gccccacgga ccccgttgtc cctgcagtgc ccataggccc gcccccggct
     1681 ggtttcagag acatcctgct gcgagagggg cctgagggct ttgctcgagc tgtgcggaac
     1741 cacccggggc tgctgctgat ggacacgacc ttcagggacg cccaccagtc actgctggcc
     1801 actcgtgtgc gcacccacga tctcaaaaag atcgccccct atgttgccca caacttcagc
     1861 aagctcttca gcatggagaa ctggggagga gccacgtttg acgtcgccat gcgcttcctg
     1921 tatgagtgcc cctggcggcg gctgcaggag ctccgggagc tcatccccaa catccctttc
     1981 cagatgctgc tgcggggggc caatgctgtg ggctacacca actacccaga caacgtggtc
     2041 ttcaagttct gtgaagtggc caaagagaat ggcatggatg tcttccgtgt gtttgactcc
     2101 ctcaactact tgcccaacat gctgctgggc atggaggcgg caggaagtgc cggaggcgtg
     2161 gtggaggctg ccatctcata cacgggcgac gtggccgacc ccagccgcac caagtactca
     2221 ctgcagtact acatgggctt ggccgaagag ctggtgcgag ctggcaccca catcctgtgc
     2281 atcaaggaca tggccgggct gctgaagccc acggcctgca ccatgctggt cagctccctc
     2341 cgggaccgct tccccgacct cccactgcac atccacaccc acgacacgtc aggggcaggc
     2401 gtggcagcca tgctggcctg tgcccaggct ggagctgatg tggtggatgt ggcagctgat
     2461 tccatgtctg ggatgacttc acagcccagc atgggggccc tggtggcctg taccagaggg
     2521 actcccctgg acacagaggt gcccatggag cgcgtgtttg actacagtga gtactgggag
     2581 ggggctcggg gactgtacgc ggccttcgac tgcacggcca ccatgaagtc tggcaactcg
     2641 gacgtgtatg aaaatgagat cccagggggc cagtacacca acctgcactt ccaggcccac
     2701 agcatggggc ttggctccaa gttcaaggag gtcaagaagg cctatgtgga ggccaaccag
     2761 atgctgggcg atctcatcaa ggtgacgccc tcctccaaga tcgtggggga cctggcccag
     2821 tttatggtgc agaatggatt gagccgggca gaggccgaag ctcaggcgga agagctgtcc
     2881 tttccccgct ccgtggtgga gttcctgcag ggctacatcg gtgtccccca tggggggttc
     2941 cccgaaccct ttcgctctaa ggtactgaag gacctgccaa gggtggaggg gcggcctgga
     3001 gcctccctcc ctcccctgga tctgcaggca ctggagaagg agctggtaga ccggcatggg
     3061 gaggaggtga cgccggaaga tgtgctctca gcagctatgt accccgatgt gtttgcccac
     3121 ttcaaggact tcactgccac ctttggcccc ctggatagcc tgaatactcg cctcttcctg
     3181 cagggaccca agatcgcaga ggagtttgag gtggagctgg agcggggcaa gacgctgcac
     3241 atcaaagccc tggccgtgag cgacctgaac cgggccggcc agaggcaggt cttctttgag
     3301 ctcaatgggc agctgcggtc catcttggtc aaggacaccc aggccatgaa ggagatgcac
     3361 ttccacccca aggccctaaa ggacgtgaag ggccagatcg gggcgcccat gcctgggaag
     3421 gtgatagaca tcaaagtggt ggcaggggcc aaggtggcca agggccagcc cctgtgtgtg
     3481 ctcagtgcca tgaagatgga gactgtggtg acctcaccca tggagggtac tgtccgcaag
     3541 gttcatgtga ccaaggacat gacactggaa ggtgacgacc tcatcctgga gatcgagtga
     3601 gaattc
//
