LOCUS       sequence-357602-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for stable integration of Renilla luciferase NMD(+) 
            PTC39 reporter at AAVS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Udy DB, Bradley RK
  TITLE     Nonsense-mediated mRNA decay uses complementary mechanisms to 
            suppress mRNA and protein accumulation.
  JOURNAL   Life Sci Alliance. 2021 Dec 8;5(3). pii: 5/3/e202101217. doi: 
            10.26508/lsa.202101217. Print 2022 Mar.
  PUBMED    34880103
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-12-8"; volume: "5(3). pii: 5/3/e202101217. 
            doi"; pages: " 10.26508/lsa.202101217. Print 2022 Mar"
COMMENT     Sequence Label: AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-A...
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..26
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    44..62
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             366..431
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agtttactcc ctatcagtga tagagaacgt atgcagactt tactccctat cagtgataga
       61 gaacgtataa ggagtttact ccctatcagt gatagagaac gtatgaccag tttactccct
      121 atcagtgata gagaacgtat ctacagttta ctccctatca gtgatagaga acgtatatcc
      181 agtttactcc ctatcagtga tagagaacgt ataagctttg cttatgtaaa ccagggcgcc
      241 tataaaagag tgctgatttt ttgagtaaac ttcaattcca caacactttt gtcttatacc
      301 aactttccgt accacttcct accctcgtaa agaattcgcg gccgcaaccg tcagaattaa
      361 ccatggacta caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg
      421 acgatgacaa gcttgcggcc gcgaattcaa cttcgaaagt ttatgatcca gaacaaagga
      481 aacggatgat aactggtccg cagtggtggg ccagatgtaa acaaatgaat gttcttgatt
      541 catttattaa ttattatgat tcagaaaaac atgcagaaaa tgctgttatt tttttacatg
      601 gtaacgcggc ctcttcttat ttatggcgac atgttgtgcc acatattgag ccagtagcgc
      661 ggtgtattat accagacctt attggtatgg gcaaatcagg caaatctggt aatggttctt
      721 ataggttact tgatcattac aaatatctta ctgcatggtt tgaacttc
//
