LOCUS       sequence-357609-       10316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding RWDD2B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: RWDD2B_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1001..2017
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1941..1963
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2025..2044
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2133..2157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3121..3145)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3165..3188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3223..3772
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3367..3384)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3594..3613
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3775..3777,3778..3780,3781..4494)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3775..3777,3778..3780,3781..4491)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3820..3841)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4081..4100)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4428..4449
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4571..5159
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4624..4644)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5042..5053)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5162..5178)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5163..5179)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5368..5601
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5673..5794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5710..5729)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5764..5783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5834..5969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5989..6008)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5990..6008)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6031..6389)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6071..6092)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6283..6302
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6416..6520
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6521..6589,6590..7381)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6739..6758)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7552..8140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8041..8060
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8315..8618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8620..8818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8773..8793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8836..9016
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9063..9188
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9685..9918
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10103..10147
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      781 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      841 ggggsgcgcg ccrggsgggg sggggsgggg sgrggggsgg ggsggggsgr ggcggagagg
      901 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
      961 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct
     1021 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     1081 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     1141 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
     1201 gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
     1261 cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     1321 ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg
     1381 ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg
     1441 gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga
     1501 gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc
     1561 gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga
     1621 gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag
     1681 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
     1741 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc
     1801 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg
     1861 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg
     1921 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     1981 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     2041 tgtgctgtct catcattttg gcaaagaatt cccctagccg cggaatctag actgccggat
     2101 cgaattgtcg acgaattcgg atctggccaa tcacaagttt gtacaaaaaa gcaggcacca
     2161 tgaaaattga gctgtccatg cagccatgga acccgggtta cagcagtgag ggggccacgg
     2221 ctcaagaaac ttacacatgt ccaaaaatga ttgagatgga gcaggcggag gcccagcttg
     2281 ctgagttaga cctgctagcc agtatgttcc ctggtgagaa tgagctcata gtgaatgacc
     2341 agctggctgt agcagaactg aaagattgta ttgaaaagaa gacaatggag gggcgatctt
     2401 caaaagtcta ctttactatc aatatgaacc tggatgtatc tgacgaaaaa atggcgatgt
     2461 tttctctggc ctgtattctt ccctttaaat acccggcagt tctgcctgaa atcactgtca
     2521 gatcagtatt attgagtaga tcccagcaga ctcagctgaa cacagatctg actgcattcc
     2581 tgcaaaaaca ttgtcatgga gatgtttgta tactgaatgc cacagagtgg gttagagaac
     2641 acgcctctgg ctatgtcagc agagatactt catcttcacc caccacagga agcacagtcc
     2701 agtcagttga cctcatcttc acgagactct ggatctacag ccatcatatc tataacaaat
     2761 gcaaaagaaa gaatattcta gagtgggcaa aggagctttc cctgtctggg tttagcatgc
     2821 ctggaaaacc tggtgttgtt tgtgtggaag gcccacaaag tgcctgtgaa gaattctggt
     2881 caagactcag aaaattaaac tggaagagaa ttttaattcg ccatcgagaa gacattcctt
     2941 ttgatggtac aaatgatgaa acggaaagac aaaggaaatt ttccattttt gaagaaaaag
     3001 tgttcagtgt taatggagcc aggggaaacc acatggactt tggtcagctc tatcagttct
     3061 taaacaccaa aggatgtggg gatgttttcc agatgttctt tggtgtagaa ggacaataga
     3121 acccagcttt cttgtacaaa gtggtgggag gaggtggatc tggcgactac aaggacgacg
     3181 atgacaagtg actcgagcgg gatcaattcc gccccccccc taacgttact ggccgaagcc
     3241 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     3301 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     3361 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     3421 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     3481 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     3541 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     3601 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     3661 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     3721 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatggtg
     3781 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3841 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3901 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3961 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     4021 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4081 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4141 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4201 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     4261 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     4321 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4381 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4441 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     4501 cgcgactcta gagtcgacct gcaggcatgc aagcttcagg tagccggcta acgttatcaa
     4561 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4621 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4681 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4741 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4801 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4861 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4921 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4981 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5041 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5101 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     5161 tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata
     5221 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     5281 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     5341 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5401 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5461 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5521 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5581 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     5641 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     5701 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5761 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     5821 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5881 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5941 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     6001 tcgtattagc atgtgagcaa aaggccagca taaatcaaaa gaatagaccg agatagggtt
     6061 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     6121 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     6181 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     6241 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     6301 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     6361 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     6421 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6481 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6541 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6601 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6661 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6721 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6781 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6841 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6901 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6961 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccgaagct
     7021 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7081 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7141 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7201 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7261 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7321 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7381 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7441 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7501 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7561 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7621 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7681 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7741 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7801 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7861 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7921 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7981 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8041 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8161 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8221 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     8281 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     8341 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     8401 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     8461 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     8521 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     8581 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     8641 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     8701 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     8761 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     8821 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8881 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8941 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9001 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     9061 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     9121 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     9181 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     9241 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     9301 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     9361 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     9421 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     9481 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     9541 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     9601 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9661 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9721 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9781 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9841 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     9901 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9961 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    10021 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    10081 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    10141 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    10201 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    10261 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggg
//
