LOCUS       sequence-35771-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Taelman VF, Dobrowolski R, Plouhinec JL, Fuentealba LC, Vorwald PP, 
            Gumper I, Sabatini DD, De Robertis EM
  TITLE     Wnt signaling requires sequestration of glycogen synthase kinase 3 
            inside multivesicular endosomes.
  JOURNAL   Cell. 2010 Dec 23. 143(7):1136-48.
  PUBMED    21183076
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Dec 23. 143(7):1136-48."
COMMENT     Sequence Label: pCS2 Smad1 EVE GM
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             134..157
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tctggtacca cgcgtatcga
      121 taagcttacc atggactaca aggacgacga tgacaagaat gtgacaagtt tattttcctt
      181 tacaagtcca gctgtgaaga gacttcttgg gtggaaacag ggcgatgaag aagaaaaatg
      241 ggcagagaaa gctgttgatg ctttggtgaa aaaactgaag aaaaagaaag gtgccatgga
      301 ggaactggaa aaggccttga gctgcccagg gcaaccgagt aactgtgtca ccattccccg
      361 ctctctggat ggcaggctgc aagtctccca ccggaaggga ctgcctcatg tcatttactg
      421 ccgtgtgtgg cgctggcccg atcttcagag ccaccatgaa ctaaaaccac tggaatgctg
      481 tgagtttcct tttggttcca agcagaagga ggtctgcatc aatccctacc actataagag
      541 agtagaaagc cctgtacttc ctcctgtgct ggttccaaga cacagcgaat ataatcctca
      601 gcacagcctc ttagctcagt tccgtaactt aggacaaaat gagcctcaca tgccactcaa
      661 cgccactttt ccagattctt tccagcaacc caacagccac ccgtttcctc actctcccaa
      721 tagcgcttac ccaaactctc ctggggcaag cagcagcgct taccctcact ctcccaccag
      781 cgctgaccc
//
