LOCUS       sequence-357716-        6895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast integrative plasmid for expressing Murine polyomavirus 
            deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 
            promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6895)
  TITLE     Plasmids for expressing MPyV VLPs with reporter protein cargo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: APEX2 VLP
FEATURES             Location/Qualifiers
     source          1..6895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1238..1255)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1238..1254)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1247..1269)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1463..1482)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1582..1604
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1642..1660)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1728..1832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1833..1901,1902..2693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2051..2070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2864..3452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3353..3372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3606..3623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3740..3761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3776..3781,3782..3799,3800..3806)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3814..3830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3819..3841
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3838..3854
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3838..3854
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(5261..5702)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(5267..5290)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    5585..5702
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 gtatatgtac agttttatat atatatatat atatatatac atatataaag aaacctgtgc
       61 gttttttgta ttttcaaata catttagttt tggcgccact tctataaaag gtctcatgat
      121 attgttactc tagagatccc aatacaacag atcacgtgat ctttttgtaa gatgaagttg
      181 aagtagtgtt gcaccgtgcc aatgcaggtg gctattagat taaatatgtg atttgttcta
      241 ttaagtttcc tgtataatta atggggagcg ctgattctct tttggtacgc ttcccatcca
      301 gcatttctgt atctttcacc ttcaacctta ggatctctac ccttggcgaa aagtcctctg
      361 ccaacaatga tgatatctga tccaccactt acaacttcgt cgacggttct gtactgctga
      421 cccaatgcat cgcctttgtc gtctaaacct acacctgggg tcatgattag ccaatcaaac
      481 ccttcttctc ttcctcccat atcgttctga gcaatgaacc caataacgaa atctttatca
      541 ctctttgcaa tatcaacggt acccttagta tattcaccgt gtgctagaga acccttggaa
      601 gacaattcag caagcatcaa taatcccctt ggttctttgg tgacctcttg cgcaccttgt
      661 ttcaagccag caacaatacc agcaccagta accccgtggg cgttggtgat atcagaccat
      721 tctgcgatac ggtaaacgcc cgatgtatat tgtaatttga ctgtgttacc gatatcggcg
      781 aattttctgt cctcaaatat caagaacttg tatttctctg ccaatgcttt caatggaacg
      841 acagtaccct cataactgaa atcatccaag atatcaacgt gtgttttcaa aaggcaaatg
      901 tatggaccca acgtttcaac aagtttcaat agctcatcag tcgaacgaac gtcaagagaa
      961 gcacacaaat tggtcttctt ttcatccatt aaacgtaaaa gtttcgatgc aaccggactt
     1021 gcatgagtct cagctctact ggtatatgat tttgtggaca tgttttatct tcgtttcctg
     1081 caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca
     1141 ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt
     1201 tcggagatta ccgaatcaaa aaaatttaaa tgaattcact ggccgtcgtt ttacaacgtc
     1261 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     1321 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     1381 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     1441 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     1501 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     1561 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     1621 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     1681 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1801 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1861 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1921 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1981 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2041 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2101 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2161 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2221 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2281 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2341 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2401 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2461 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2521 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2581 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2641 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2701 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2761 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3001 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3601 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3661 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     3721 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     3781 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     3841 gaaacagcta tgaccatgat tacgccaagc ttgcatgcat ttaaatcatt acgaccgaga
     3901 ttcccgggta ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat
     3961 gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc
     4021 cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt
     4081 cctactagtg cggccgctta attaccaggg aatacagttt tagttttacc gaatctatct
     4141 acatatcttg tcatgtcagg gtcacctgga actggttcag taccatcgta aactctaact
     4201 tcttcaactt gtgtgttttc accttccatt ggttgacctt gaacttgtgg taacatatta
     4261 ttaaacaaag aagaaatcaa agaagccatt gggtatggat ttttaaccca tctttttctt
     4321 aaagtaatct tgaagtatct tggcaaacct ctccaatgat gaacatcgta gtttcttgta
     4381 actctccaac ccataatatc aacgcaagat aagtacaaac cttcaccttt acataatgga
     4441 ccaacaccat tttcatctaa caaaacagtt gtcaaagtgt tagtgaattg caaaactggt
     4501 ggagttgtag taccaccagt gtaattaccg aagtatcttg tgttttcatt tttagctgga
     4561 tctggatgcc aaatttcaac tgggtacatg ccatccttat ccaatttagc ctttgaaatt
     4621 gggttcaaaa cttgatcctt attaaccata tctttcttag tgattgtttt aatagtaaca
     4681 acaccttctt ccttgtactt agttcttgca tctgtaacta aaccttgcaa atccaatggt
     4741 tcaccaccaa cagcaaaaac atgatattgt gaaccttcaa ctggagtaga aatacccttt
     4801 gtgttaacag tatctgttgg cttattaaaa ccatgaacat ccaacaaaga accagaacca
     4861 acaacttctg ttttaactga aactgcttcc cacatttgca aagtatcaca agtcaaatct
     4921 tcgttcaaca ttggtaattg caattttgcc atagaccaag ttggtaatgt attattacct
     4981 ggtgaatctt cagtatcaga tgtagccaaa ttaatacctc ttgaccaacc gtaatattga
     5041 ccaccttcag ttaaagattc tggtgttggt ggttgaccca ttcttgggtt caaaaatgct
     5101 tcgatttcag taacagaatc tggacctgta accaaatcca aaacttccat accaccttta
     5161 atcaataact taggaactgg agctggtcta gggcaagcct ttgtgcattt ggtttcgcat
     5221 ttacttacac ctgaagccat tttgctagct atagtttttt ctccttgacg ttaaagtata
     5281 gaggtatatt aacaattttt tgttgatact tttatgacat ttgaataaga agtaatacaa
     5341 accgaaaatg ttgaaagtat tagttaaagt ggttatgcag cttttgcatt tatatatctg
     5401 ttaatagatc aaaaatcatc gcttcgctga ttaattaccc cagaaataag gctaaaaaac
     5461 taatcgcatt attatcctat ggttgttaat ttgattcgtt gatttgaagg tttgtggggc
     5521 caggttactg ccaatttttc ctcttcataa ccataaaagc tagtattgta gaatctttat
     5581 tgttcggagc agtgcggcgc gaggcacatc tgcgtttcag gaacgcgacc ggtgaagacc
     5641 aggacgcacg gaggagagtc ttccgtcgga gggctgtcgc ccgctcggcg gcttctaatc
     5701 cgtacttcaa tatagcaatg agcagttaag cgtattactg aaagttccaa agagaaggtt
     5761 tttttaggct aagataatgg ggctctttac atttccacaa catataagta agattagata
     5821 tggatatgta tatggtggta ttgccatgta atatgattat taaacttctt tgcgtccatc
     5881 caaaaaaaaa agtaagaatt tttgaaaatt caatataaat cgataaaatg ggtaatggtg
     5941 gtccaactcc agctgctcat attcaagatg aatctggtga agttattaaa ttttatcaag
     6001 ctcaagttgt ttctcatcaa agagttactc cagattggat gttgccattg attttgggtt
     6061 tgtatggtga catcactcca ggtggtggtg gttctggtgg cggtggctcc ggtggcggcg
     6121 gttctatggg taaatcatac cctactgttt cagcagacta tcaagacgct gtcgaaaaag
     6181 caaaaaagaa gttgagaggt ttcattgctg aaaaaagatg cgctccatta atgttgagat
     6241 tagcttttca ttcagctggt acttttgata aaggtacaaa aacaggtggt ccatttggta
     6301 ctattaaaca tccagctgaa ttagctcatt ctgcaaataa tggtttggat atagcagtta
     6361 gattattaga accattaaaa gctgagtttc caattttgtc ttatgctgat ttttatcaat
     6421 tggctggtgt tgttgctgtc gaagtcactg gaggaccaaa agttccattc catccaggta
     6481 gagaagataa accagaacct ccacctgaag gtagattgcc agatccaaca aaaggctctg
     6541 atcatctaag agatgttttc ggtaaagcta tgggattgac agaccaagat atagttgcat
     6601 tgtctggtgg tcatacaatt ggagctgcac acaaagaaag atcaggattc gaaggaccat
     6661 ggacatctaa tcctttgata tttgataact cttattttac tgagttgtta tctggtgaaa
     6721 aagaaggttt gttacaatta ccatccgata aagctttatt atctgatccc gtctttcgtc
     6781 cacttgttga taaatatgca gctgatgagg acgcattttt tgccgattat gcagaggccc
     6841 accagaaatt gtcagaatta ggatttgcag atgcttaaat agattaattt aaaca
//
