LOCUS       sequence-357717-        7794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged 
            with VP2C (GAL10 promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7794)
  TITLE     Plasmids for expressing MPyV VLPs with reporter protein cargo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Akaluc VLP
FEATURES             Location/Qualifiers
     source          1..7794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(235..251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(235..251)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(248..270)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    259..275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(283..289,290..307,308..313))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    328..349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(466..483)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(637..1225)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(717..736)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1396..2187,2188..2256))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2019..2038
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2257..2361)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2429..2447
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2485..2507)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2607..2626
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2820..2842
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2834..2851
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2835..2851
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6181..6622
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    6181..6298
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     6593..6616
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 ctagtaggac tatttgcaaa gggaagggat gctaaggtag agggtgaacg ttacagaaaa
       61 gcaggctggg aagcatattt gagaagatgc ggccagcaaa actaaaaaac tgtattataa
      121 gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata tcagttatta
      181 cccgggaatc tcggtcgtaa tgatttaaat gcatgcaagc ttggcgtaat catggtcata
      241 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      301 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      361 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      421 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      481 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      541 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      601 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      661 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      721 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      781 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      841 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      901 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      961 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1021 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     1081 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1141 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1201 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1261 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1321 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1381 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1441 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1501 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     1561 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1621 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1681 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1741 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1801 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     1861 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     1921 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     1981 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2041 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2101 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2161 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2221 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2281 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2341 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     2401 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     2461 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     2521 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2581 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     2641 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     2701 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2761 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2821 ccagtcacga cgttgtaaaa cgacggccag tgaattcatt taaatttttt tgattcggta
     2881 atctccgagc agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc
     2941 atatgtggtg ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca
     3001 aaaacctgca ggaaacgaag ataaaacatg tccacaaaat catataccag tagagctgag
     3061 actcatgcaa gtccggttgc atcgaaactt ttacgtttaa tggatgaaaa gaagaccaat
     3121 ttgtgtgctt ctcttgacgt tcgttcgact gatgagctat tgaaacttgt tgaaacgttg
     3181 ggtccataca tttgcctttt gaaaacacac gttgatatct tggatgattt cagttatgag
     3241 ggtactgtcg ttccattgaa agcattggca gagaaataca agttcttgat atttgaggac
     3301 agaaaattcg ccgatatcgg taacacagtc aaattacaat atacatcggg cgtttaccgt
     3361 atcgcagaat ggtctgatat caccaacgcc cacggggtta ctggtgctgg tattgttgct
     3421 ggcttgaaac aaggtgcgca agaggtcacc aaagaaccaa ggggattatt gatgcttgct
     3481 gaattgtctt ccaagggttc tctagcacac ggtgaatata ctaagggtac cgttgatatt
     3541 gcaaagagtg ataaagattt cgttattggg ttcattgctc agaacgatat gggaggaaga
     3601 gaagaagggt ttgattggct aatcatgacc ccaggtgtag gtttagacga caaaggcgat
     3661 gcattgggtc agcagtacag aaccgtcgac gaagttgtaa gtggtggatc agatatcatc
     3721 attgttggca gaggactttt cgccaagggt agagatccta aggttgaagg tgaaagatac
     3781 agaaatgctg gatgggaagc gtaccaaaag agaatcagcg ctccccatta attatacagg
     3841 aaacttaata gaacaaatca catatttaat ctaatagcca cctgcattgg cacggtgcaa
     3901 cactcacttc aacttcatct tacaaaagat cacgtgatct gttgtattgg gatctctaga
     3961 gtaacaatat catgagacct tttatagaag tggcgccaaa actaaatgta tttgaaaata
     4021 caaaaaacgc acaggtttct ttatatatgt atatatatat atatatatat ataaaactgt
     4081 acatatactg tttaaattaa tctatctaca cagcgatctt accgtccttc ttagccttgg
     4141 tcaggatctc tctaatcttc ctagcgtcca gcttaccggt agatcctcta ggaacttcgt
     4201 ccacgaacac aacaccacct ctaagctttt tagcggtggt cacctgagat gccacgtagt
     4261 ccacgatttc tttctcggtc atagtcttac cgtgctcaag cacaacaaca gcagcaggaa
     4321 gttcaccagc gtcatcatca ggcagacctg caacaccagc atcgaagatg tacgggtgct
     4381 gaagaagaat accctccaac tcagctggtg ccacctggta acccttgtac ttgatcagag
     4441 acttcagccg gtccacgatg aagaagtgct catcttcatc ccagtaagcg atatcaccgc
     4501 tgtgcagcca accatcctta tcgatgagag cgttggtggc ctcaggattg ttcacgtagc
     4561 cagacatgat catagggccc ctaacgcaaa gctcgcctct ctgattaaca ccaagggtct
     4621 taccggtcac caaatccaca accttagcct cgaagaatgg caccacctta ccaacagaac
     4681 caggctttct atcgccctca ggggtaatca tcacagcgtt ggtagtctcg gtcaaaccgt
     4741 aaccttgcct aataccagga agcctgaacc tcttagcgac agcttctcca acctctttag
     4801 aaagcggagc accaccagaa gcaatctcac gcagagagct aaggtcgtac ttgtcgatca
     4861 gggtgctctt agcaaggcaa gaaaacaaag tcggcaccag cagagcagac tggatcttgt
     4921 aatcctgaag gctccgcagg aacaactctt cctcaaaccg gtacatcagc acaaccctga
     4981 aaccgcagat caggtaaccc aaggtggtga acataccgaa accgtgatgg aaaggcacca
     5041 cgctaagaat agcggtgtca ggaatgttct ggtagccgaa gataggatcc ctagcatgag
     5101 agaacctcac gcaagcagtt ctatgaggaa gtgcaacacc cttaggcaga ccggtagaac
     5161 cagagctgtt catgatcaga gcaatggtct tgtcccgatc gaagctctca ggcacgaaat
     5221 cgtactcgtt gaaagacggc ggaaggtgag aagtcacgaa ggtgtacata gactggaagc
     5281 cctggtaatc ggtcttgctg tccatgatga tgatcttccg gatgatcggc agcttcttct
     5341 gcacgttcag aacctttctc aggcccttct tagacacgaa caccacagta ggctgagaaa
     5401 tgcccatgct gttaagcagt tccctctcgt tgtagatgtc gttggcagga gcaacagcaa
     5461 caccaatgaa aagagcgccc agcacaggca tgaagaactg agagctattc tcgctgcaca
     5521 caacgatccg gtggttggta ttaagaccgt accttctcat ggcctcagcc agtctaacag
     5581 acatctcgaa gtactcggcg taggtaacgt caacctgaat gtgagcatcg gtgaaagcga
     5641 tagcaccagg cacaagagcg tacctcttca tagccttgtg aagctgctca ccagcagtac
     5701 catcctcaag agggtaaaat ggagcaggcc ccttcttgat gttcttggca tcttccatag
     5761 aaccgccgcc accggagcca ccgccaccag aaccaccacc acctggagtg atgtcaccat
     5821 acaaacccaa aatcaatggc aacatccaat ctggagtaac tctttgatga gaaacaactt
     5881 gagcttgata aaatttaata acttcaccag attcatcttg aatatgagca gctggagttg
     5941 gaccaccatt acccatttta tcgatttata ttgaattttc aaaaattctt actttttttt
     6001 tggatggacg caaagaagtt taataatcat attacatggc attaccacca tatacatatc
     6061 catatctaat cttacttata tgttgtggaa atgtaaagag ccccattatc ttagcctaaa
     6121 aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta tattgaagta
     6181 cggattagaa gccgccgagc gggcgacagc cctccgacgg aagactctcc tccgtgcgtc
     6241 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
     6301 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
     6361 tggccccaca aaccttcaaa ttaacgaatc aaattaacaa ccataggatg ataatgcgat
     6421 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
     6481 aacagatata taaatggaaa agctgcataa ccactttaac taatactttc aacattttca
     6541 gtttgtatta cttcttattc aaatgtcata aaagtatcaa caaaaaattg ttaatatacc
     6601 tctatacttt aacgtcaagg agaaaaaact atagctagca aaatggcttc aggtgtaagt
     6661 aaatgcgaaa ccaaatgcac aaaggcttgc cctagaccag ctccagttcc taagttattg
     6721 attaaaggtg gtatggaagt tttggatttg gttacaggtc cagattctgt tactgaaatc
     6781 gaagcatttt tgaacccaag aatgggtcaa ccaccaacac cagaatcttt aactgaaggt
     6841 ggtcaatatt acggttggtc aagaggtatt aatttggcta catctgatac tgaagattca
     6901 ccaggtaata atacattacc aacttggtct atggcaaaat tgcaattacc aatgttgaac
     6961 gaagatttga cttgtgatac tttgcaaatg tgggaagcag tttcagttaa aacagaagtt
     7021 gttggttctg gttctttgtt ggatgttcat ggttttaata agccaacaga tactgttaac
     7081 acaaagggta tttctactcc agttgaaggt tcacaatatc atgtttttgc tgttggtggt
     7141 gaaccattgg atttgcaagg tttagttaca gatgcaagaa ctaagtacaa ggaagaaggt
     7201 gttgttacta ttaaaacaat cactaagaaa gatatggtta ataaggatca agttttgaac
     7261 ccaatttcaa aggctaaatt ggataaggat ggcatgtacc cagttgaaat ttggcatcca
     7321 gatccagcta aaaatgaaaa cacaagatac ttcggtaatt acactggtgg tactacaact
     7381 ccaccagttt tgcaattcac taacactttg acaactgttt tgttagatga aaatggtgtt
     7441 ggtccattat gtaaaggtga aggtttgtac ttatcttgcg ttgatattat gggttggaga
     7501 gttacaagaa actacgatgt tcatcattgg agaggtttgc caagatactt caagattact
     7561 ttaagaaaaa gatgggttaa aaatccatac ccaatggctt ctttgatttc ttctttgttt
     7621 aataatatgt taccacaagt tcaaggtcaa ccaatggaag gtgaaaacac acaagttgaa
     7681 gaagttagag tttacgatgg tactgaacca gttccaggtg accctgacat gacaagatat
     7741 gtagatagat tcggtaaaac taaaactgta ttccctggta attaagcggc cgca
//
