LOCUS       sequence-357719-        6653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia 
            luciferase tagged with VP2C (GAL10 promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6653)
  TITLE     Plasmids for expressing MPyV VLPs with reporter protein cargo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GLuc VLP
FEATURES             Location/Qualifiers
     source          1..6653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(253..269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(253..269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(266..288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    277..293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(301..307,308..325,326..331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    346..367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(484..501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(655..1243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(735..754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1414..2205,2206..2274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2037..2056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2275..2379)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2447..2465
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2503..2525)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2625..2644
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2838..2860
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2852..2869
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2853..2869
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5058..5499
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    5058..5175
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     5470..5493
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 ggtaattaag cggccgcact agtaggacta tttgcaaagg gaagggatgc taaggtagag
       61 ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac
      121 taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt
      181 taattatatc agttattacc cgggaatctc ggtcgtaatg atttaaatgc atgcaagctt
      241 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      301 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      361 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      421 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      481 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      541 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      601 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      661 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      721 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      781 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      841 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      901 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      961 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1021 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1081 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1141 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1201 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1261 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1321 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1381 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1441 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1501 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1561 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1621 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1681 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1741 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1801 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1861 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1921 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1981 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2041 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2101 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2161 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2221 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2281 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2341 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2401 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2461 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2521 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2581 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2641 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2701 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2761 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2821 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcattta
     2881 aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt
     2941 agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta
     3001 acccaactgc acagaacaaa aacctgcagg aaacgaagat aaaacatgtc cacaaaatca
     3061 tataccagta gagctgagac tcatgcaagt ccggttgcat cgaaactttt acgtttaatg
     3121 gatgaaaaga agaccaattt gtgtgcttct cttgacgttc gttcgactga tgagctattg
     3181 aaacttgttg aaacgttggg tccatacatt tgccttttga aaacacacgt tgatatcttg
     3241 gatgatttca gttatgaggg tactgtcgtt ccattgaaag cattggcaga gaaatacaag
     3301 ttcttgatat ttgaggacag aaaattcgcc gatatcggta acacagtcaa attacaatat
     3361 acatcgggcg tttaccgtat cgcagaatgg tctgatatca ccaacgccca cggggttact
     3421 ggtgctggta ttgttgctgg cttgaaacaa ggtgcgcaag aggtcaccaa agaaccaagg
     3481 ggattattga tgcttgctga attgtcttcc aagggttctc tagcacacgg tgaatatact
     3541 aagggtaccg ttgatattgc aaagagtgat aaagatttcg ttattgggtt cattgctcag
     3601 aacgatatgg gaggaagaga agaagggttt gattggctaa tcatgacccc aggtgtaggt
     3661 ttagacgaca aaggcgatgc attgggtcag cagtacagaa ccgtcgacga agttgtaagt
     3721 ggtggatcag atatcatcat tgttggcaga ggacttttcg ccaagggtag agatcctaag
     3781 gttgaaggtg aaagatacag aaatgctgga tgggaagcgt accaaaagag aatcagcgct
     3841 ccccattaat tatacaggaa acttaataga acaaatcaca tatttaatct aatagccacc
     3901 tgcattggca cggtgcaaca ctacttcaac ttcatcttac aaaaagatca cgtgatctgt
     3961 tgtattggga tctctagagt aacaatatca tgagaccttt tatagaagtg gcgccaaaac
     4021 taaatgtatt tgaaaataca aaaaacgcac aggtttcttt atatatgtat atatatatat
     4081 atatatataa aactgtacat atactgttta aattaatcta tttaatcacc cccagctcct
     4141 ttaattttgt cgacttgtcc ttgtatttta ctggcaaaag tagcgcatct ttgtggtagc
     4201 cacttcttta gtaagtctga gcattgcaca ttcgccaacc cttttaagca gcctgtagtg
     4261 cagtctacac acaaatctac ttgtgctata aattgctcca taggctctaa atctttgaaa
     4321 cccgggattt cagggatgtc cactattgct tctccgatcc cgccctgagc tgattccttg
     4381 tctccctcat acgtgtggca acgcccgggg ataaactttt tcattttagg cgtacacttg
     4441 atatgactta agcaaattaa acaacctcta gtacaacctg cctttcttgc attcgcctcc
     4501 atctctttca atacttcaag cggtaacttc tttccaggaa gttttccacg atctgcgtct
     4561 aggtcggtcg tggcaaaatt actagcgaca gcgacaatgt taaagtcctc attgttctcc
     4621 gtgggtttcg ccatagaacc gccgccaccg gagccaccgc caccagaacc accaccacct
     4681 ggagtgatgt caccatacaa acccaaaatc aatggcaaca tccaatctgg agtaactctt
     4741 tgatgagaaa caacttgagc ttgataaaat ttaataactt caccagattc atcttgaata
     4801 tgagcagctg gagttggacc accattaccc attttatcga tttatattga attttcaaaa
     4861 attcttactt ttttttttgg atggacgcaa agaagtttaa taatcatatt acatggcaat
     4921 accaccatat acatatccat atctaatctt acttatatgt tgtggaaatg taaagagccc
     4981 cattatctta gcctaaaaaa accttctctt tggaactttc agtaatacgc ttaactgctc
     5041 attgctatat tgaagtacgg attagaagcc gccgagcggg cgacagccct ccgacggaag
     5101 actctcctcc gtgcgtcctg gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg
     5161 cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg
     5221 aaaaattggc agtaacctgg ccccacaaac cttcaaatca acgaatcaaa ttaacaacca
     5281 taggataata atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg
     5341 atgatttttg atctattaac agatatataa atgcaaaagc tgcataacca ctttaactaa
     5401 tactttcaac attttcggtt tgtattactt cttattcaaa tgtcataaaa gtatcaacaa
     5461 aaaattgtta atatacctct atactttaac gtcaaggaga aaaaactata gctagcaaaa
     5521 tggcttcagg tgtaagtaaa tgcgaaacca aatgcacaaa ggcttgccct agaccagctc
     5581 cagttcctaa gttattgatt aaaggtggta tggaagtttt ggatttggtt acaggtccag
     5641 attctgttac tgaaatcgaa gcatttttga acccaagaat gggtcaacca ccaacaccag
     5701 aatctttaac tgaaggtggt caatattacg gttggtcaag aggtattaat ttggctacat
     5761 ctgatactga agattcacca ggtaataata cattaccaac ttggtctatg gcaaaattgc
     5821 aattaccaat gttgaacgaa gatttgactt gtgatacttt gcaaatgtgg gaagcagttt
     5881 cagttaaaac agaagttgtt ggttctggtt ctttgttgga tgttcatggt tttaataagc
     5941 caacagatac tgttaacaca aagggtattt ctactccagt tgaaggttca caatatcatg
     6001 tttttgctgt tggtggtgaa ccattggatt tgcaaggttt agttacagat gcaagaacta
     6061 agtacaagga agaaggtgtt gttactatta aaacaatcac taagaaagat atggttaata
     6121 aggatcaagt tttgaaccca atttcaaagg ctaaattgga taaggatggc atgtacccag
     6181 ttgaaatttg gcatccagat ccagctaaaa atgaaaacac aagatacttc ggtaattaca
     6241 ctggtggtac tacaactcca ccagttttgc aattcactaa cactttgaca actgttttgt
     6301 tagatgaaaa tggtgttggt ccattatgta aaggtgaagg tttgtactta tcttgcgttg
     6361 atattatggg ttggagagtt acaagaaact acgatgttca tcattggaga ggtttgccaa
     6421 gatacttcaa gattacttta agaaaaagat gggttaaaaa tccataccca atggcttctt
     6481 tgatttcttc tttgtttaat aatatgttac cacaagttca aggtcaacca atggaaggtg
     6541 aaaacacaca agttgaagaa gttagagttt acgatggtac tgaaccagtt ccaggtgacc
     6601 ctgacatgac aagatatgta gatagattcg gtaaaactaa aactgtattc cct
//
