LOCUS       sequence-35773-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Tantama M, Hung YP, Yellen G
  TITLE     Imaging Intracellular pH in Live Cells with a Genetically Encoded 
            Red Fluorescent Protein Sensor.
  JOURNAL   J Am Chem Soc. 2011 Jul 6. 133(26):10034-7.
  PUBMED    21631110
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2011 Jul 6. 133(26):10034-7."
COMMENT     Sequence Label: pRsetB-pHRed
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             38..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             join(68..70,71..73,74..733)
                     /codon_start=1
                     /product="red fluorescent protein that serves as a 
                     ratiometric pH sensor (Tantama et al., 2011)"
                     /label=pHRed
                     /note="contains an A213S mutation relative to the parental 
                     mKeima protein"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEISIARHSLLG
                     "
     primer_bind     complement(846..864)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      860..907
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgaaacat caccatcacc atcaccatgg
       61 agctagcatg gtttccgtta tcgcaaagca aatgacctat aaagtttata tgtctggcac
      121 cgttaacggc cactacttcg aggttgaagg tgacggcaag ggcaagcctt acgagggcga
      181 gcagaccgtt aagctgaccg ttaccaaagg cggcccactg cctttcgcct gggatatcct
      241 gtcccctcag ctccaatacg gctccatccc tttcaccaaa taccccgagg acatccctga
      301 ctactttaag cagagcttcc cagagggcta cacctgggag cgctctatga acttcgagga
      361 cggcgcagtt tgtaccgttt ccaacgacag ctccatccag ggtaactgct tcatctacaa
      421 cgttaagatt tccggcgaga acttcccacc taacggccca gttatgcaga agaaaaccca
      481 aggctgggag cccagcaccg aacgcctgtt tgcccgcgac ggtatgctga ttggtaacga
      541 ttatatggcc ctgaaactgg aaggcggcgg tcactacctg tgcgagttca agagcactta
      601 caaggctaaa aagcctgttc gcatgcctgg ccgccacgaa atcgaccgca agctggatgt
      661 tacctctcac aaccgcgact acaccagcgt tgagcagtgt gaaatctcta tcgcccgcca
      721 ctctctgctg gggacaggca actccgccga cggtggtggc tctggtggtt ctagatgact
      781 cgaggggccc aagcttgatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      841 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      901 ttttttgctg aaaggagaac tatatccgg
//
