LOCUS       sequence-357736-        5647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter gene with yeast recombination site within 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: p{3P3-mTurquoise::KD,attB}
FEATURES             Location/Qualifiers
     source          1..5647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    303..336
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    377..446
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(665..685)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(665..683)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(704..720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(704..720)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(717..739)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    728..744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(752..758,759..776,777..782))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    797..818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(935..952)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1106..1694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1186..1205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1865..2656,2657..2725))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2488..2507
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2726..2830)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2857..3312
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2944..2963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3154..3175
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3438..3460
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3452..3469
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3453..3469
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3479..3498
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3479..3497
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3796..3805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4555..4579
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4677..4701)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctctgggacg atgagtcatt cctttcgggg ccacagcata atcgttgcca gctcaccgaa
       61 atggtgactt catttcttaa ctgccgtcaa gcatgcgatt gtacatacat acatatttat
      121 atatgtacat atttatgtga ctatggtagg tcgatataat agcaatcaac gcaagcaaat
      181 gtgtcagtcc tgcttacagg aacgattcta tttagtaatt ttcgttgtat aaagtaatta
      241 tgtatgtatg taagccccat aaatctgaaa caattaggca aaaccatgcg aagctctcta
      301 gtataacttc gtataatgta tgctatacga agttctcgag ggggggcccg gtaccgtcga
      361 cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc
      421 cgggcgcgta ctccacctca cccatctggt ccatcatgat gaacgggtcg aggtggcggt
      481 agttgatccc ggcgaacgcg cggcgcgccg ggaagccctc gccctcgaaa ccgctgggcg
      541 cggtggtcac ggtgagcacg ggacgtgcga cggcgtcggc gggtgcggat acgcggggca
      601 gcgtcagcgg gttctcgacg gtcacggcgg gcatgtcgac actagtggta cccagctttt
      661 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
      721 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      781 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      841 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      901 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      961 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1741 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1801 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1861 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1921 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1981 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2041 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2101 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2161 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2221 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2281 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2341 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2401 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2461 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2521 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2581 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2641 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2701 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2761 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2821 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     2881 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2941 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3001 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3061 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3121 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3181 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     3241 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3301 cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     3361 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     3421 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     3481 atacgactca ctatagggcg aattggagct ccaccgcggt ggccatatgc aatggttaat
     3541 tcgagctcgc ccggggatct aattcaatta gagactaatt caattagagc taattcaatt
     3601 aggatccaag cttatcgatt tcgaaccctc gaccgccgga gtataaatag aggcgcttcg
     3661 tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta
     3721 agcaaataaa caagcgcagc tgaacaagct aaacaatcgg ggtaccgcta gagtcgacgg
     3781 tacgatccac cggtcgccac catggtgtcc aagggagagg agctgttcac cggagtcgtg
     3841 ccaatcctcg tcgagctgga tggtgacgtg aacggccata agttctccgt gtccggtgag
     3901 ggcgaaggtg acgccaccta cggaaagctg accctgaagt tcatctgcac caccggtaag
     3961 ctgcccgtcc cctggcccac cctggtcacc accctgtcct ggggagtgca gtgcttcgct
     4021 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc agaaggatac
     4081 gttcaggagc gcaccatctt cttcaaggac gatggcaact acaagacccg cgccgaggtg
     4141 aagttcgagg gcgataccct ggtcaaccgt atcgagctga agggcatcga cttcaaggag
     4201 gacggcaaca tcttgggtca caagctggag tacaactaca ttagcgacaa cgtgtacatc
     4261 accgctgata agcagaagaa cggcatcaag gctaacttca agatccgtca caacatcgag
     4321 gatggcggcg tccagctggc tgatcactac cagcagaaca cccccattgg tgatggtccc
     4381 gtgctgctgc ccgataatca ctacctgtcc acccagtcca agctctccaa ggtgagtagt
     4441 acatgtgcat acatcttaag ttcacttgat ctataggaac tgcgattgca acatcaaatt
     4501 gtctgcggcg tgagaactgc gacccacaaa aatcccaaac cgcggccgtt tatcaccact
     4561 ttgtacaaga aagctgggtc ggcgcgccca cccttaaacg atatcagaca tttgtctgat
     4621 aatgaagcat tatcagacaa atgtctgata tcgtttggtg aagggggcgg ccgcggagcc
     4681 tgcttttttg tacaaacttg tgataaacga attcttgaag acgaaagggc acaaacaaat
     4741 agtgacacga aacagattat tctggtagct gttctcgcta tataagacaa tttttgagat
     4801 catatcatga tcaagacatc taaaggcatt cattttcgac tatattcttt tttacaaaaa
     4861 atataacaac cagatatttt aagctgatcc tagatgcaca aaaaataaat aaaagtataa
     4921 acctacttcg taggatactt cggggtactt tttgttcggg gttagatgag cataacgctt
     4981 gtagttgata tttgagatcc cctatcattg caggacccaa acgagaagcg tgatcacatg
     5041 gtcctcctcg agttcgtgac cgccgccggc atcaccctgg gcatggacga gctgtacaag
     5101 taagtatagt gccttgaccg agctcgccca ctaagcgtcg cgccacttca acgctcgatg
     5161 ggagcgtcat tggtgggcgg ggtaaccgtc gaaatcagtg tttacgcttc caatcgcaac
     5221 aaaaaattca ctgcaacact gaaaagcata cgaaaacgat gaagattgta cgagaaacca
     5281 taaagtattt tatccacaaa gacacgtata gcagaaaagc caagttaact cggcgataag
     5341 ttgtgtacac aagaataaaa tcggccagat tcagtgttgt cagaaataag aaaaccccac
     5401 tatgtttttc tttgcctttt ctttctccca gcgatcattc atttcgtggt gaaagaacgg
     5461 ggtcattgca cggagtttcg actgcgggaa agcagagctg ccgttcactt cgtctataat
     5521 tagcgctttc tattttcccc gattcgggcc gctgctgcgc ttttccgcct gctgtttgtg
     5581 gcaagtgtag cagcaggctg tgcacgcagt gtggcatgca cttggctttc caccgttggt
     5641 atcgatt
//
