LOCUS       sequence-357738-        6182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter gene with yeast recombination site within 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: p{Mhc-F4-678-DsRed::KD,attB}
FEATURES             Location/Qualifiers
     source          1..6182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    516..540
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(638..662)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2356..2389
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2430..2499
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2718..2738)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2718..2736)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2757..2773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2757..2773)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2770..2792)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2781..2797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2805..2811,2812..2829,2830..2835))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2850..2871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2988..3005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3159..3747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3239..3258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3918..4709,4710..4778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4541..4560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4779..4883)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4910..5365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4997..5016)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5207..5228
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5491..5513
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5505..5522
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5506..5522
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5532..5551
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5532..5550
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tatatatgca aagcattctg atatatatat ataagtggta tacatataca tattcgtatc
       61 ggatccgtga tctatattta ggattaaaac cctcgaccgc cggagtataa atagaggcgc
      121 ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa
      181 gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tcgggcggcc gcactagagc
      241 cggtcgccac catgaggtct tccaagaatg ttatcaagga gttcatgagg tttaaggttc
      301 gcatggaagg aacggtcaat gggcacgagt ttgaaataga aggcgaagga gaggggaggc
      361 catacgaagg ccacaatacc gtaaagctta aggtgagtag tacatgtgca tacatcttaa
      421 gttcacttga tctataggaa ctgcgattgc aacatcaaat tgtctgcggc gtgagaactg
      481 cgacccacaa aaatcccaaa ccgcggccgt ttatcaccac tttgtacaag aaagctgggt
      541 cggcgcgccc acccttaaac gatatcagac atttgtctga taatgaagca ttatcagaca
      601 aatgtctgat atcgtttggt gaagggggcg gccgcggagc ctgctttttt gtacaaactt
      661 gtgataaacg aattcttgaa gacgaaaggg cacaaacaaa tagtgacacg aaacagatta
      721 ttctggtagc tgttctcgct atataagaca atttttgaga tcatatcatg atcaagacat
      781 ctaaaggcat tcattttcga ctatattctt ttttacaaaa aatataacaa ccagatattt
      841 taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc gtaggatact
      901 tcggggtact ttttgttcgg ggttagatga gcataacgct tgtagttgat atttgagatc
      961 ccctatcatt gcaggtaacc aaggggggac ctttgccatt tgcttgggat attttgtcac
     1021 cacaatttca gtatggaagc aaggtatatg tcaagcaccc tgccgacata ccagactata
     1081 aaaagctgtc atttcctgaa ggatttaaat gggaaagggt catgaacttt gaagacggtg
     1141 gcgtcgttac tgtaacccag gattccagtt tgcaggatgg ctgtttcatc tacaaggtca
     1201 agttcattgg cgtgaacttt ccttccgatg gacctgttat gcaaaagaag acaatgggct
     1261 gggaagccag cactgagcgt ttgtatcctc gtgatggcgt gttgaaagga gagattcata
     1321 aggctctgaa gctgaaagac ggtggtcatt acctagttga attcaaaagt atttacatgg
     1381 caaagaagcc tgtgcagcta ccagggtact actatgttga ctccaaactg gatataacaa
     1441 gccacaacga agactataca atcgttgagc agtatgaaag aaccgaggga cgccaccatc
     1501 tgttccttta gcggccatcg aattcgagct cgcccactaa gcgtcgcgcc acttcaacgc
     1561 tcgatgggag cgtcattggt gggcggggta accgtcgaaa tcagtgttta cgcttccaat
     1621 cgcaacaaaa aattcactgc aacactgaaa agcatacgaa aacgatgaag attgtacgag
     1681 aaaccataaa gtattttatc cacaaagaca cgtatagcag aaaagccaag ttaactcggc
     1741 gataagttgt gtacacaaga ataaaatcgg ccagattcag tgttgtcaga aataagaaaa
     1801 ccccactatg tttttctttg ccttttcttt ctcccagcga tcattcattt cgtggtgaaa
     1861 gaacggggtc attgcacgga gtttcgactg cgggaaagca gagctgccgt tcacttcgtc
     1921 tataattagc gctttctatt ttccccgatt cgggccgctg ctgcgctttt ccgcctgctg
     1981 tttgtggcaa gtgtagcagc aggctgtgca cgcagtgtgg catgcacttg gctttccacc
     2041 gttggtatcg attctctggg acgatgagtc attcctttcg gggccacagc ataatcgttg
     2101 ccagctcacc gaaatggtga cttcatttct taactgccgt caagcatgcg attgtacata
     2161 catacatatt tatatatgta catatttatg tgactatggt aggtcgatat aatagcaatc
     2221 aacgcaagca aatgtgtcag tcctgcttac aggaacgatt ctatttagta attttcgttg
     2281 tataaagtaa ttatgtatgt atgtaagccc cataaatctg aaacaattag gcaaaaccat
     2341 gcgaagctct ctagtataac ttcgtataat gtatgctata cgaagttctc gagggggggc
     2401 ccggtaccgt cgacgatgta ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg
     2461 cccttgggct ccccgggcgc gtactccacc tcacccatct ggtccatcat gatgaacggg
     2521 tcgaggtggc ggtagttgat cccggcgaac gcgcggcgcg ccgggaagcc ctcgccctcg
     2581 aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg
     2641 gatacgcggg gcagcgtcag cgggttctcg acggtcacgg cgggcatgtc gacactagtg
     2701 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     2761 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2821 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2881 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2941 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3001 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3061 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3901 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3961 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4021 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4081 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4141 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4201 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4261 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4321 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4381 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4441 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4501 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4561 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4621 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4681 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4741 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4801 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4861 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     4921 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4981 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5041 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5101 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5161 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5221 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5281 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5341 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
     5401 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     5461 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     5521 gtgagcgcgc gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc
     5581 gctttaatat actcgaattc gttgtcggct catatacatg ggcgaaataa tttcgaatat
     5641 gttttaaaaa taaccaaaga cattagaaag agatcgccaa tacttataca ttatgtctat
     5701 gtgtgccatg tggtagcatg agccaaaaag cttctcgaaa ttacgaatta catatagacg
     5761 aatatgtatg tgactttagt ttcgaaataa tttcgagaat tttaaaaata acgcattcgt
     5821 taaaagttcg cgtcaattcg aatcgaattt tcgattgtcg atttagtgtg gattgtcgaa
     5881 aatcgttccg ccttcgaagt ttactgaaag gaatcattgc gatctcgtga attgcttgta
     5941 tgagaacacg ccaccatatc gagatacctg ttcaaaatat cgaaatcgcg ttccaaacgg
     6001 tagtagttac cagttgagtg agttgtggtg acagtttaac ctctctacat ttgtaataat
     6061 aagcattgtt ttggcgtatc aatagtctgt agattttttc acaaagaaac ctggagaaat
     6121 gtacacaaat atctaaatga aaattggcgt tacgtaacaa ataccaacgc cccgaaaatc
     6181 cg
//
