LOCUS       sequence-357747-       11163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HsTNRC6A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11163)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 11163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: pAc5.1B-EGFP-HsTNRC6A_L
FEATURES             Location/Qualifiers
     source          1..11163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2575..2596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2836..2855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3183..3204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    7470..7481
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             8414..8455
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8465..8482
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8505..8522)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8555..8689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8579..8598)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8633..8652
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9290..9878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9370..9389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10049..10840,10841..10909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10672..10691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10910..11014)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11082..11100
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2881 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2941 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3001 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3121 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3241 tacaagccaa gcttgatgga tgctgattct gcctccagtt ctgaatcaga gagaaacatc
     3301 actatcatgg cttcagggaa cacaggtggt gaaaaagatg gccttcggaa tagcactgga
     3361 cttggttccc aaaacaagtt tgtagttggt agcagcagca ataatgtggg ccatggaagt
     3421 agtactgggc catggggttt ttcccatgga gccataataa gcacatgtca ggtctctgtg
     3481 gatgctcctg aaagcaaatc tgaaagtagc aacaatagaa tgaatgcttg gggcactgta
     3541 agttcttcat caaatggagg gttaaatcca agcactttga attcagctag caaccatggt
     3601 gcctggccag tattagagaa caatggactt gccctaaaag ggcctgtagg gagtggtagt
     3661 tctggcatta atattcagtg cagtactata ggccagatgc ctaacaatca gagtattaac
     3721 tctaaagtga gtggtggttc tacccatggt acctggggaa gccttcagga aacttgtgaa
     3781 tctgaagtaa gtggtacaca gaaggtttca ttcagtggtc aacctcaaaa tattaccact
     3841 gaaatgactg gaccaaataa cactactaac tttatgacct ctagtttacc aaactccggt
     3901 tcagtgcaga ataatgagct gcctagtagt aacacagggg cctggcgtgt gagcacaatg
     3961 aatcatcctc agatgcaggc tccatcaggt atgaatggca cttccctttc tcaccttagc
     4021 aatggagagt caaaaagtgg aggctcttat ggtactacat ggggtgccta tggttctaat
     4081 tactctggag acaaatgttc aggccctaat ggccaagcta atggtgacac tgtgaatgca
     4141 actctaatgc agcctggcgt aaatggtcct atgggcacta actttcaagt taacacaaac
     4201 aaaggaggtg gtgtgtggga atctggtgca gcaaactccc agagtacatc atggggaagt
     4261 ggaaatggca caaattctgg aggaagtcga agaggatggg gaacccctgc acaaaacact
     4321 ggcactaatt tacccagcgt tgagtggaac aaactgccta gcaatcagca ttccaatgat
     4381 agtgcaaatg gcaatggtaa gacgtttaca aatggatgga aatctactga ggaagaggat
     4441 cagggttctg ccacatctca gacaaatgag caaagcagtg tgtgggccaa aacaggaggt
     4501 acagtggaga gcgatggtag tacagaaagc actggacgcc ttgaggaaaa aggaactggg
     4561 gaaagtcaga gtagagacag aagaaaaatt gatcagcaca cattactcca aagcattgta
     4621 aacagaactg acttagatcc acgtgtcctg tccaactctg gttggggaca gactcctatt
     4681 aagcagaata ctgcctggga tacagaaaca tcacctagag gggaacgaaa gactgacaat
     4741 gggacagagg cctggggaag ctctgcaaca cagactttta actcaggggc atgtatagat
     4801 aagactagcc ctaatggtaa tgatacctca tctgtatcag ggtggggcga tcccaaacct
     4861 gctctgaggt ggggagattc caaaggctca aactgccagg gggggtggga agatgattct
     4921 gctgctacag gaatggtcaa gagcaatcag tgggggaatt gcaaagagga gaaggctgca
     4981 tggaatgact cgcaaaagaa taaacaggga tggggtgatg gacaaaaatc aagccaaggg
     5041 tggtctgttt ctgccagtga taactgggga gaaacttcaa ggaataacca ttggggtgag
     5101 gccaataaga aatccagctc aggaggtagt gacagtgaca ggtccgtttc cggttggaac
     5161 gaacttggta aaactagttc tttcacttgg ggaaacaaca taaatccaaa taattcatca
     5221 ggatgggatg aatcttctaa acctactcct tcccagggat ggggagaccc tccaaagtct
     5281 aatcagtctc taggttgggg agattcgtca aagccagtca gctctccaga ctggaacaag
     5341 caacaagaca ttgttggatc ttggggaatc ccaccagcta caggcaaacc tcctggtaca
     5401 ggctggctgg ggggacctat accagcccca gcaaaagaag aagaacccac aggctgggag
     5461 gaaccatccc cagaatctat acgtcgcaaa atggagattg atgatggaac ttcagcttgg
     5521 ggagatccaa gcaaatacaa ctacaaaaat gtgaacatgt ggaacaaaaa cgtcccaaat
     5581 ggcaacagcc gttcagacca gcaagcacag gtacatcagc tgctaacgcc tgcaagtgcc
     5641 atctcaaaca aagaggcaag cagtggctct ggctggggtg agccctgggg ggagccttct
     5701 actccagcca caactgtgga taatggtact tcagcatggg gtaagcccat agacagtggt
     5761 cccagctggg gggaacccat tgctgcggca tccagcacat ccacgtgggg ctccagctct
     5821 gttggtccac aagcattaag caaatctggg ccaaaatcta tgcaagatgg ctggtgtggt
     5881 gatgatatgc cattgcctgg aaatcgcccc actggctggg aagaggaaga ggatgtggag
     5941 attggaatgt ggaatagtaa ttcatctcaa gagcttaact catctttaaa ttggccacca
     6001 tatacaaaga aaatgtcatc gaagggtctg agtggcaaaa aaaggagaag ggaaggggga
     6061 atgatgaaag gtggaaacaa acaagaagaa gcgtggataa atccatttgt taaacagttt
     6121 tcaaacatca gtttttcgag agactcacca gaggaaaatg tacaaagcaa taagatggac
     6181 ctttctggag gaatgttaca agacaaacga atggagatag ataaacatag cctaaatatt
     6241 ggtgattaca atcgaacggt cgggaaaggc cctggttctc ggcctcagat ttccaaagag
     6301 tcttccatgg agcgcaatcc ttattttgat aaggatggca ttgtagcaga tgaatcccaa
     6361 aacatgcagt ttatgtccag tcaaagcatg aaacttcccc cttcaaatag tgcactacct
     6421 aaccaggccc ttggctccat agcagggctg ggtatgcaaa acttgaattc tgttagacag
     6481 aatggcaatc ccagtatgtt tggtgttgga aacacagcag cacaaccccg gggcatgcag
     6541 cagcctccag cacaacctct tagttcatct cagcctaatc tccgtgctca agtgcctcct
     6601 ccattactct cccctcaggt tccagtttca ttgctgaagt atgcaccaaa caacggtggc
     6661 ctgaatccac tctttggccc tcaacaggta gccatgctga accagctatc ccagctaaac
     6721 cagctttctc agatctccca gttacagcga ttgttagcgc agcagcaaag ggcgcagagt
     6781 cagagaagcg tgccttctgg gaaccggccg cagcaagacc agcagggtcg acctcttagt
     6841 gtgcagcagc aaatgatgca acaatctcgt caacttgatc caaacctgtt ggtgaagcag
     6901 cagactccac catctcagca gcagccactc catcagccag ccatgaagtc tttccttgac
     6961 aatgtcatgc cccacactac acctgagctg caaaaagggc catcaccaat aaatgctttc
     7021 agcaacttcc ctataggctt gaactcaaac ttgaatgtaa atatggatat gaacagtatt
     7081 aaagagccac agtcaagact aaggaagtgg acgacagtgg gcagcatttc tgtgaacaca
     7141 tctttggatc aaaactccag caaacatggt gctatttcaa gtggtttcag gctggaagag
     7201 tctccatttg ttccctatga ctttatgaac agcagtactt caccagccag tcctccaggt
     7261 tcaataggag atggctggcc acgtgccaaa tcgcctaacg gctctagcag tgttaattgg
     7321 ccaccagaat ttcgtcctgg tgagccatgg aaaggttatc caaacattga ccctgaaact
     7381 gacccttacg tcactcctgg cagtgtcata aacaatcttt caattaatac tgtgcgggaa
     7441 gttgaccacc tcagggacag gaacagtggg tcatcctcat ccttgaacac cacgctgcct
     7501 tcaactagtg cctggtcatc cattcgtgcc tccaactaca acgttcccct cagcagtaca
     7561 gcacaaagca cttcagccag aaatagtgat tccaaattga catggtctcc tggttcagtt
     7621 acaaacacct ctctggctca tgagctgtgg aaggtccctt tgccacctaa aaacatcact
     7681 gctccgtccc gcccacctcc gggactgact ggtcagaagc cacccttgtc tacgtgggat
     7741 aattctcccc ttcgtatagg tggaggatgg ggaaattctg acgccagata taccccaggt
     7801 tccagctggg gtgagagcag ctcagggaga ataacaaatt ggcttgttct aaaaaacctt
     7861 acacctcaga tcgatggctc aactctgcgc actctgtgca tgcagcacgg cccgctgatc
     7921 acattccacc tgaacctccc tcacggaaat gctctggtcc gctacagttc aaaagaagag
     7981 gtagtggagg cacaaaagtc tctgcacatg tgtgtactgg ggaacactac tattcttgct
     8041 gagtttgcca gtgaagagga gatcagtcgt ttctttgcac aaagccagtc tctgacccct
     8101 tctcccggct ggcagtctct cgggtccagc cagagccggc tgggctccct cgactgttcc
     8161 cactcattct ccagccggac cgatctcaat cactggaatg gtgctgggct gtcgggaact
     8221 aactgtggag accttcacgg cacttcactc tgggggaccc cgcattattc cacaagcctg
     8281 tggggtcccc caagcagcag cgacccccga ggaattagca gcccatctcc cattaacgct
     8341 tttctttctg ttgaccacct gggtgggggt ggagagtcca tgtaactcga gtctagaggg
     8401 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     8461 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
     8521 taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa gatacattga
     8581 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     8641 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     8701 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     8761 aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc ttgagagcct
     8821 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     8881 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     8941 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     9001 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     9061 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     9121 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     9181 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     9241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9721 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
    10381 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10441 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10501 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10561 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10621 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
    10681 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10741 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10801 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10861 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10921 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10981 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    11041 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    11101 cctttcgtct tcaagaatta attctatatt ctaaaaacac aaatgatact tctaaaaaaa
    11161 aat
//
