LOCUS       sequence-357758-        5620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter gene with yeast recombination site within 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: p{3P3-mTurquoise::FRT-RC,attB}
FEATURES             Location/Qualifiers
     source          1..5620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(135..926,927..995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     758..777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(996..1100)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1127..1582
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1214..1233)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1424..1445
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1708..1730
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1722..1739
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1723..1739
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1749..1768
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1749..1767
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2066..2075
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2828..2852
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2869..2902)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(2923..2947)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    4193..4226
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4267..4336
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(4555..4575)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4555..4573)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4594..4610)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4594..4610)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4607..4629)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4618..4634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4642..4648,4649..4666,4667..4672))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4687..4708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4825..4842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4996..5584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5076..5095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
       61 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      121 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      181 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
      241 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
      301 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
      361 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
      421 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
      481 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
      541 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
      601 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
      661 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
      721 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
      781 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
      841 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
      901 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
      961 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1021 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1081 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
     1141 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     1201 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     1261 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     1321 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     1381 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     1441 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     1501 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     1561 aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc
     1621 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1681 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     1741 agcgcgcgta atacgactca ctatagggcg aattggagct ccaccgcggt ggccatatgc
     1801 aatggttaat tcgagctcgc ccggggatct aattcaatta gagactaatt caattagagc
     1861 taattcaatt aggatccaag cttatcgatt tcgaaccctc gaccgccgga gtataaatag
     1921 aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa
     1981 gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatcgg ggtaccgcta
     2041 gagtcgacgg tacgatccac cggtcgccac catggtgtcc aagggagagg agctgttcac
     2101 cggagtcgtg ccaatcctcg tcgagctgga tggtgacgtg aacggccata agttctccgt
     2161 gtccggtgag ggcgaaggtg acgccaccta cggaaagctg accctgaagt tcatctgcac
     2221 caccggtaag ctgcccgtcc cctggcccac cctggtcacc accctgtcct ggggagtgca
     2281 gtgcttcgct cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2341 agaaggatac gttcaggagc gcaccatctt cttcaaggac gatggcaact acaagacccg
     2401 cgccgaggtg aagttcgagg gcgataccct ggtcaaccgt atcgagctga agggcatcga
     2461 cttcaaggag gacggcaaca tcttgggtca caagctggag tacaactaca ttagcgacaa
     2521 cgtgtacatc accgctgata agcagaagaa cggcatcaag gctaacttca agatccgtca
     2581 caacatcgag gatggcggcg tccagctggc tgatcactac cagcagaaca cccccattgg
     2641 tgatggtccc gtgctgctgc ccgataatca ctacctgtcc acccagtcca agctctccaa
     2701 ggacgtgagt agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt
     2761 gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcggcc
     2821 gtttatcacc actttgtaca agaaagctgg gtcggcgcgc ccacccttga agttcctata
     2881 ctttctagag aataggaact tcggtgaagg gggcggccgc ggagcctgct tttttgtaca
     2941 aacttgtgat aaacgaattc ttgaagacga aagggcacaa acaaatagtg acacgaaaca
     3001 gattattctg gtagctgttc tcgctatata agacaatttt tgagatcata tcatgatcaa
     3061 gacatctaaa ggcattcatt ttcgactata ttctttttta caaaaaatat aacaaccaga
     3121 tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct acttcgtagg
     3181 atacttcggg gtactttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg
     3241 agatccccta tcattgcagc caaacgagaa gcgtgatcac atggtcctcc tcgagttcgt
     3301 gaccgccgcc ggcatcaccc tgggcatgga cgagctgtac aagtaagtat agtgccttga
     3361 ccgagctcgc ccactaagcg tcgcgccact tcaacgctcg atgggagcgt cattggtggg
     3421 cggggtaacc gtcgaaatca gtgtttacgc ttccaatcgc aacaaaaaat tcactgcaac
     3481 actgaaaagc atacgaaaac gatgaagatt gtacgagaaa ccataaagta ttttatccac
     3541 aaagacacgt atagcagaaa agccaagtta actcggcgat aagttgtgta cacaagaata
     3601 aaatcggcca gattcagtgt tgtcagaaat aagaaaaccc cactatgttt ttctttgcct
     3661 tttctttctc ccagcgatca ttcatttcgt ggtgaaagaa cggggtcatt gcacggagtt
     3721 tcgactgcgg gaaagcagag ctgccgttca cttcgtctat aattagcgct ttctattttc
     3781 cccgattcgg gccgctgctg cgcttttccg cctgctgttt gtggcaagtg tagcagcagg
     3841 ctgtgcacgc agtgtggcat gcacttggct ttccaccgtt ggtatcgatt ctctgggacg
     3901 atgagtcatt cctttcgggg ccacagcata atcgttgcca gctcaccgaa atggtgactt
     3961 catttcttaa ctgccgtcaa gcatgcgatt gtacatacat acatatttat atatgtacat
     4021 atttatgtga ctatggtagg tcgatataat agcaatcaac gcaagcaaat gtgtcagtcc
     4081 tgcttacagg aacgattcta tttagtaatt ttcgttgtat aaagtaatta tgtatgtatg
     4141 taagccccat aaatctgaaa caattaggca aaaccatgcg aagctctcta gtataacttc
     4201 gtataatgta tgctatacga agttctcgag ggggggcccg gtaccgtcga cgatgtaggt
     4261 cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta
     4321 ctccacctca cccatctggt ccatcatgat gaacgggtcg aggtggcggt agttgatccc
     4381 ggcgaacgcg cggcgcgccg ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac
     4441 ggtgagcacg ggacgtgcga cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg
     4501 gttctcgacg gtcacggcgg gcatgtcgac actagtggta cccagctttt gttcccttta
     4561 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4621 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4681 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4741 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4801 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4861 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4921 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5401 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5521 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5581 tcaagaagat cctttgatct tttctacggg gtctgacgct
//
