LOCUS       sequence-357768-        5587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter gene with yeast recombination site within 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: p{3P3-dsRed::KD-RC,attB}
FEATURES             Location/Qualifiers
     source          1..5587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(179..970,971..1039))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     802..821
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1040..1144)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1171..1626
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1258..1277)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1468..1489
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1752..1774
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1766..1783
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1767..1783
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1793..1812
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1793..1811
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1844..1860
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1844..1860
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    2364..2388
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2486..2510)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    4204..4237
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4278..4347
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(4566..4586)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4566..4584)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4605..4621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4605..4621)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4618..4640)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4629..4645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4653..4659,4660..4677,4678..4683))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4698..4719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4836..4853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(5007..5587,1..8))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5087..5106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
       61 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      121 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      181 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
      241 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      301 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
      361 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      421 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      481 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      541 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      601 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      661 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      721 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      781 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      841 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      901 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      961 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1021 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1081 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1141 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     1201 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     1261 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     1321 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     1381 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     1441 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     1501 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     1561 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     1621 caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     1681 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     1741 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     1801 ctcactatag ggcgaattgg agctccaccg cggtggcggc cgctctagaa ctagtggatc
     1861 ccccggggat ctaattcaat tagagactaa ttcaattaga gctaattcaa ttaggatcca
     1921 agcttatcga tttcgaaccc tcgaccgccg gagtataaat agaggcgctt cgtctacgga
     1981 gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata
     2041 aacaagcgca gctgaacaag ctaaacaatc gggcggccgc actagagccg gtcgccacca
     2101 tgaggtcttc caagaatgtt atcaaggagt tcatgaggtt taaggttcgc atggaaggaa
     2161 cggtcaatgg gcacgagttt gaaatagaag gcgaaggaga ggggaggcca tacgaaggcc
     2221 acaataccgt aaagcttaag gtgagtagta catgtgcata catcttaagt tcacttgatc
     2281 tataggaact gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa
     2341 atcccaaacc gcggccgttt atcaccactt tgtacaagaa agctgggtcg gcgcgcccac
     2401 ccttaaacga tatcagacat ttgtctgata atgcttcatt atcagacaaa tgtctgatat
     2461 cgtttggtga agggggcggc cgcggagcct gcttttttgt acaaacttgt gataaacgaa
     2521 ttcttgaaga cgaaagggca caaacaaata gtgacacgaa acagattatt ctggtagctg
     2581 ttctcgctat ataagacaat ttttgagatc atatcatgat caagacatct aaaggcattc
     2641 attttcgact atattctttt ttacaaaaaa tataacaacc agatatttta agctgatcct
     2701 agatgcacaa aaaataaata aaagtataaa cctacttcgt aggatacttc ggggtacttt
     2761 ttgttcgggg ttagatgagc ataacgcttg tagttgatat ttgagatccc ctatcattgc
     2821 aggtaaccaa ggggggacct ttgccatttg cttgggatat tttgtcacca caatttcagt
     2881 atggaagcaa ggtatatgtc aagcaccctg ccgacatacc agactataaa aagctgtcat
     2941 ttcctgaagg atttaaatgg gaaagggtca tgaactttga agacggtggc gtcgttactg
     3001 taacccagga ttccagtttg caggatggct gtttcatcta caaggtcaag ttcattggcg
     3061 tgaactttcc ttccgatgga cctgttatgc aaaagaagac aatgggctgg gaagccagca
     3121 ctgagcgttt gtatcctcgt gatggcgtgt tgaaaggaga gattcataag gctctgaagc
     3181 tgaaagacgg tggtcattac ctagttgaat tcaaaagtat ttacatggca aagaagcctg
     3241 tgcagctacc agggtactac tatgttgact ccaaactgga tataacaagc cacaacgaag
     3301 actatacaat cgttgagcag tatgaaagaa ccgagggacg ccaccatctg ttcctttagc
     3361 ggccatcgaa ttcgagctcg cccactaagc gtcgcgccac ttcaacgctc gatgggagcg
     3421 tcattggtgg gcggggtaac cgtcgaaatc agtgtttacg cttccaatcg caacaaaaaa
     3481 ttcactgcaa cactgaaaag catacgaaaa cgatgaagat tgtacgagaa accataaagt
     3541 attttatcca caaagacacg tatagcagaa aagccaagtt aactcggcga taagttgtgt
     3601 acacaagaat aaaatcggcc agattcagtg ttgtcagaaa taagaaaacc ccactatgtt
     3661 tttctttgcc ttttctttct cccagcgatc attcatttcg tggtgaaaga acggggtcat
     3721 tgcacggagt ttcgactgcg ggaaagcaga gctgccgttc acttcgtcta taattagcgc
     3781 tttctatttt ccccgattcg ggccgctgct gcgcttttcc gcctgctgtt tgtggcaagt
     3841 gtagcagcag gctgtgcacg cagtgtggca tgcacttggc tttccaccgt tggtatcgat
     3901 tctctgggac gatgagtcat tcctttcggg gccacagcat aatcgttgcc agctcaccga
     3961 aatggtgact tcatttctta actgccgtca agcatgcgat tgtacataca tacatattta
     4021 tatatgtaca tatttatgtg actatggtag gtcgatataa tagcaatcaa cgcaagcaaa
     4081 tgtgtcagtc ctgcttacag gaacgattct atttagtaat tttcgttgta taaagtaatt
     4141 atgtatgtat gtaagcccca taaatctgaa acaattaggc aaaaccatgc gaagctctct
     4201 agtataactt cgtataatgt atgctatacg aagttctcga gggggggccc ggtaccgtcg
     4261 acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc
     4321 ccgggcgcgt actccacctc acccatctgg tccatcatga tgaacgggtc gaggtggcgg
     4381 tagttgatcc cggcgaacgc gcggcgcgcc gggaagccct cgccctcgaa accgctgggc
     4441 gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc
     4501 agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga cactagtggt acccagcttt
     4561 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     4621 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4681 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4741 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4801 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5581 aaaaaag
//
